Results 21 to 30 of about 95,582 (265)

Evaluation of Histomorphometric Changes in Tissue Architecture in Relation to Alteration in Fixation Protocol – An Invitro Study [PDF]

Journal of Clinical and Diagnostic Research, 2016
Introduction: Preparation of good tissue specimens for microscopy requires complete fixation. No ideal fixative has been found till date, with every fixative showing advantages and disadvantages. Appropriate fixation is required to maintain clear and
Pallav Singhal, Narendra Nath Singh, Gadiputi Sreedhar, Sumita Banerjee, Manu Batra, Anu Garg   +5 more
doaj   +1 more source

A Comparative Double-blind Study to Evaluate the Efficacy of Coconut Water and Honey as Cytological Fixatives for Oral Smears

Contemporary Clinical Dentistry
Introduction: Ninety-five percent ethanol is the routinely used and standard cytological fixative. However, commercially available ethanol is expensive and not freely available in some institutions. There is always a search for an ideal alternative.
Bharadwaj Bordoloi, Aanchal Tandon, Arunima Goswami, Debojyoti Roy, Abhinav Srivastava, Apoorva Sharma   +5 more
doaj   +1 more source

Impact of fixations on anti-genetic determinants of CD4, CD68, and Pax8 immunohistochemistry: A comparative study of NBF, Zink formalin, and Bouin's fluid [PDF]

Iraqi Journal of Veterinary Sciences
Fixation inhibits autolysis and is an essential step in disease. Our work examined how neutral buffer NBF (NBF), zinc formalin (ZF), and Bouin's fluid (BF) affected tissue specific CD4, CD68, and Pax8 anti-genetic determinants.
Amani A. Alayash, Firas M. Abed
doaj   +1 more source

Cytocentrifuged biopsy fixative preparation: A simple cost-effective technique facilitating microscopic diagnosis of lumen-dwelling intestinal parasites

Indian Journal of Pathology and Microbiology, 2017
Objective: Direct microscopic visualization is the most specific method for detecting intestinal parasites and is commonly achieved by stool examination or mucosal biopsy.
Prashant Joshi, Prasenjit Das, Venkateswaran Iyer, Siddharth Datta Gupta   +3 more
doaj   +1 more source

Evaluation of the Histological Staining Performance of Rat Eyeball Sections Prepared Using a Self-Developed Fixative

Shiyan dongwu yu bijiao yixue
ObjectiveTo compare the histological staining performance of four various tissue fixatives, including a self-developed fixative, for preparing paraffin sections of rat eyeball tissue.MethodsTwenty 5-week-old male Sprague-Dawley (SD) rats were randomly ...
TANG Xiaohang, GU Yingmin, LÜ Yangyang, HUANG Mingshu, TIAN Xuesong   +4 more
doaj   +1 more source

Flow Enabled Target Capture Halbach‐based magnetic enrichment increases circulating tumor cell capture from blood in metastatic cancer patients

Molecular Oncology, EarlyView.
Pair‐wise comparison of the CellSearch and FETCH enrichment technologies for circulating tumor cells (CTCs) from metastatic breast, prostate, and small cell lung cancer patients shows an increased capture of CTCs using FETCH enrichment. The clinical implementation of circulating tumor cells (CTCs) as a predictive tool for therapy efficacy in the ...
Michiel Stevens, Anouk Mentink, Tom Niessink, Frank Coumans, Leonie Mekenkamp, Ruchi Bansal, Jeroen Hiltermann   +6 more
wiley   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Qualitative analysis of histochemical and immunohistochemical properties of ethanol, glycerol, and formalin fixative

Asian Journal of Medical Sciences
Background: Fixation is essential in histopathology to preserve tissue integrity before analysis, with neutral buffered formalin being the gold standard for decades because of its effective protein cross-linking properties.
Buvaneswari V , Deepadevi G , Peter Samidoss A , Muthu Prabha MS , Vinoth Kumar V   +4 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Comparison of the Fixation Effects of Six Composite Fixatives on Retinal Tissue of Golden Hamsters

Shiyan dongwu yu bijiao yixue
Objective To compare the effects of six composite fixatives on paraffin sections of golden hamster retinal tissue, and to optimize the fixation methods for retinal tissue paraffin sections of golden hamsters.
WU Haifeng, ZHOU Xiaojiang, LI Chenjiang, LI Huaiyin, GAO Ming   +4 more
doaj   +1 more source