Human umbilical cord blood cells suffer major modification by fixatives and anticoagulants
Frontiers in Physiology, 2023 Introduction: Developing techniques for the tagless isolation of homogeneous cell populations in physiological-like conditions is of great interest in medical research. A particular case is Gravitational Field-Flow Fractionation (GrFFF), which can be run
Roberta Danusso +10 more
doaj +1 more source
Antibodies validated for routinely processed tissues stain frozen sections unpredictably
BioTechniques, 2021 Background: Antibody validation for tissue staining is required for reproducibility; criteria to ensure validity have been published recently. The majority of these recommendations imply the use of routinely processed (formalin-fixed, paraffin-embedded ...
Maddalena M Bolognesi +5 more
doaj +1 more source
A pipeline for targeted metagenomics of environmental bacteria. [PDF]
, 2020 BackgroundMetagenomics and single cell genomics provide a window into the genetic repertoire of yet uncultivated microorganisms, but both methods are usually taxonomically untargeted.
Bowers, Robert M +7 more
core +2 more sources
Transit Fixatives: An Innovative Study [PDF]
Journal of Clinical and Diagnostic Research, 2015 Background: Universally accepted fixative is 10% formalin which has been used for preserving the tissues and their architecture. In certain conditions, formalin might not be readily available for immediate fixation. We here by explore more economical,
Rajanikanth M +4 more
doaj +1 more source
Viruses, 2019 Background: To date, there is no licensed vaccine available to prevent respiratory syncytial virus (RSV) infection. The valuable pre-fusion conformation of the fusion protein (pre-F) is prone to lose high neutralizing antigenic sites.
Wei Zhang +14 more
doaj +1 more source
Evaluation of Histomorphometric Changes in Tissue Architecture in Relation to Alteration in Fixation Protocol – An Invitro Study [PDF]
Journal of Clinical and Diagnostic Research, 2016 Introduction: Preparation of good tissue specimens for microscopy requires complete fixation. No ideal fixative has been found till date, with every fixative showing advantages and disadvantages. Appropriate fixation is required to maintain clear and
Pallav Singhal +5 more
doaj +1 more source
Fixation in Form-Acetic allows hyaluronic acid detection in mouse ovaries
Reproduction and Fertility, 2022 To visualise tissues to determine the presence of disease or simply to understand anatomy, it is important to preserve fresh tissue. Fixatives are chemical solutions that preserve tissues to enable microscopic evaluation.
R Appeltant, B V Adeniran, S A Williams
doaj +1 more source
Improved ultrastructure of marine invertebrates using non-toxic buffers [PDF]
PeerJ, 2016 Many marine biology studies depend on field work on ships or remote sampling locations where sophisticated sample preservation techniques (e.g., high-pressure freezing) are often limited or unavailable.
Jacqueline Montanaro +2 more
doaj +2 more sources
Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test [PDF]
, 1985 A method for the determination of circulating immune complexes (CIC) was adapted for flow cytometric analysis. Human granulocytes were used to phagocytose IgG-bearing CIC of serum from systemic lupus erythematosus (SLE) patients.
Barnett +20 more
core +3 more sources
DNA extraction from formalin-fixed Franciscana tissues [PDF]
, 2002 The present paper reports the extraction of DNA from formalin-fixed Pontoporia blainvillei tissues. Following the Vachot and Monerot (1996) protocol, fragmented DNA (300-700bp) was extracted from more than 95% of liver and muscle samples.
Bastida, R. +2 more
core +2 more sources