Results 141 to 150 of about 36,842 (272)

A robust method for autofluorescence-free immunofluorescence using high-speed fluorescence lifetime imaging microscopy

Scientific Reports
Autofluorescence from endogenous biomolecules presents a significant challenge in immunofluorescence microscopy, often severely hindering the detection of specific fluorescence signals.
Wonsang Hwang, Tyler McPartland, Sinyoung Jeong, Conor L. Evans   +3 more
doaj   +1 more source

In vivo label-free structural and biochemical imaging of coronary arteries using an integrated ultrasound and multispectral fluorescence lifetime catheter system

Scientific Reports, 2017
Existing clinical intravascular imaging modalities are not capable of accurate detection of critical plaque pathophysiology in the coronary arteries.
Julien Bec, Jennifer E. Phipps, Dimitris Gorpas, Dinglong Ma, Hussain Fatakdawala, Kenneth B. Margulies, Jeffrey A. Southard, Laura Marcu   +7 more
doaj   +1 more source

Cell Mechanics in Cancer: Integrating Mechanotransduction Pathways Within the Tumor Microenvironment

Journal of Cellular Physiology, Volume 241, Issue 5, May 2026.
ABSTRACT Single‐cell mechanical properties such as stiffness, elasticity, and viscosity, are crucial in governing biological processes like migration, proliferation, and differentiation. In cancer, the mechanical properties of cells undergo significant alterations, which contribute to tumor growth, metastasis, and resistance to therapy.
Merve Sevgi, Yağmur Işık, Caner Karaca, Esmahan Çağlar, Hasan Berkay Abdioğlu, Ferican Zendel, Yasemin Başbınar, Hüseyin Üvet   +7 more
wiley   +1 more source

Deep Learning Integration in Optical Microscopy: Advancements and Applications

Microscopy Research and Technique, Volume 89, Issue 5, Page 791-814, May 2026.
It explores the integration of DL into optical microscopy, focusing on key applications including image classification, segmentation, and computational reconstruction. ABSTRACT Optical microscopy is a cornerstone imaging technique in biomedical research, enabling visualization of subcellular structures beyond the resolution limit of the human eye ...
Pottumarthy Venkata Lahari, Sagnika Dutta, H. Deeksha, Samreen A. Patel, Budheswar Dehury, Nirmal Mazumder   +5 more
wiley   +1 more source

The Dual Role of Methyl Caffeate: Growth Inhibition of Pseudomonas syringae and Induction of Resistance to Bacterial Speck Disease in Tomato

Molecular Plant Pathology, Volume 27, Issue 5, May 2026.
Methyl caffeate exhibits dual functionality against Pseudomonas syringae pv. tomato. It directly disrupts bacterial membrane integrity, induces reactive oxygen species accumulation, and inhibits flagellar motility and the type III secretion system. Concurrently, it primes plant defence by activating defence‐related gene expression and enzyme activities,
Yarui Yang, Jikai Yu, Jinxi Yang, Yulin Wang, Wenrui Hou, Dongfang Zhang, Jianquan Wang, Wenfeng Nie, Yan Zhang, Xiaoyun Wang, Xiuming Li, Qinghua Shi   +11 more
wiley   +1 more source

Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2

The Plant Journal, Volume 126, Issue 3, May 2026.
SUMMARY Plasma membranes (PM) contain myriads of diverse nanodomains that correspond to nanometric scale structures enriched in specific lipids and proteins acting as signaling/regulation hubs involved in diverse biological processes. So far, how PM nanodomains are formed and maintained in plant cells remains largely unknown.
Michal Daněk, Omar Hdedeh, Jesús Amo, Jessica Boutet, Michaela Neubergerová, Héla Safi, Anas Abuzeineh, Amanda Martín‐Barranco, Jean‐Bernard Fiche, Caroline Mercier, Baldwin Dumortier, Gabriel Krouk, Marcelo Nollmann, Roman Pleskot, Yohann Boutté, Véronique Santoni, Sébastien Mongrand, Alexandre Martinière, Enric Zelazny   +18 more
wiley   +1 more source

Characterisation of red fluorescent protein FLIM properties and comparison with novel StayGold live cell imaging [PDF]

BIO Web of Conferences
Tripal Philipp, Said Hanan, Schmid Benjamin, Palmisano Ralph   +3 more
doaj   +1 more source

Multispectral multiplexed confocal FLIM for live cell imaging

JPhys Photonics
Spectrally resolved fluorescence lifetime imaging (FLIM) with high spatial precision offers comprehensive information on species localization and behavior.
Morgan Richards, Elizabeth Julianna Osterlund, Andrea Rhikkaella Buendia, Nikolina Malic, Ray Truant, Qiyin Fang   +5 more
doaj   +1 more source

VPS13 has an important role in female germline development in Arabidopsis

The Plant Journal, Volume 126, Issue 3, May 2026.
SUMMARY The female germline precursor cell, known as the megaspore mother cell (MMC), differentiates and develops within the ovule through a complex genetic network, including post‐transcriptional and translational regulation mediated by miRNA. A key player in restricting MMC development to a single cell within the nucellus is the AUXIN RESPONSE FACTOR
Rosanna Petrella, Camilla Banfi, Vicente Balanzà, Alex Cavalleri, Fabio Radi, Letizia Cornaro, Riccardo Capelli, Carlo Camilloni, Matteo Chiara, Peter J. van Dijk, Diana Rigola, Rik Op den Camp, Mara Cucinotta, Lucia Colombo   +13 more
wiley   +1 more source

Efficient wide-field FLIM

, 2018
Nanosecond temporal resolution enables new methods for wide-field imaging like time-of-flight, gated detection, and fluorescence lifetime. The optical efficiency of existing approaches, however, presents challenges for low-light applications common to fluorescence microscopy and single-molecule imaging.
Bowman, Adam J., Klopfer, Brannon B., Juffmann, Thomas, Kasevich, Mark A.   +3 more
openaire   +2 more sources