Results 101 to 110 of about 5,219,938 (357)

Calcium‐sensing receptor induces the apoptosis of chondrocytes in cooperation with phosphate transporter

FEBS Open Bio, EarlyView.
Excess Ca2+ ions activate the Calcium‐Sensing Receptor (CaSR), which subsequently drives the uptake of excess inorganic phosphate (Pi) via the Pi transporter (Pit−1) in chondrocytes. This mechanism causes a toxic increase in intracellular Pi concentration, ultimately leading to chondrocyte apoptosis and pathological mineralization. Excess extracellular
Sachie Nakatani, Hiroya Ueda, Ayumi Kawata, Yuki Sato, Kotomi Inomata, Hiroshi Mano, Masahiro Wada, Kenji Kobata   +7 more
wiley   +1 more source

Best Practices for Preparing a Single Cell Suspension from Solid Tissues for Flow Cytometry

Cytometry Part A, 2018
Preparing a single cell suspension is a critical step in any solid tissue flow cytometry experiment. Tissue dissection, enzymatic digestion, and mechanical dissociation are three significant steps leading to the degradation of the extracellular matrix ...
A. Reichard, K. Asosingh
semanticscholar   +1 more source

Navigating new norms: a systematic review of factors for the development of effective digital tools in higher education

FEBS Open Bio, EarlyView.
What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman, Jessica Haines, Connie Pritchard, Stephen Rutherford, Nigel Francis   +4 more
wiley   +1 more source

Dual-view transport of intensity phase imaging flow cytometry

, 2023
Aihui Sun, Yaxi Li, Pengfei Zhu, Xiaoliang He, Zhilong Jiang, Yan Kong, Cheng Liu, Shouyu Wang   +7 more
openalex   +1 more source

P1633: LOW SERUM BDNF LEVELS ARE ASSOCIATED WITH LOWER PLATELETS’ CD62P REACTIVITY AND INCREASED BLEEDING TENDENCY IN PATIENTS WITH GAUCHER DISEASE

HemaSphere, 2022
D. Azoulay, M. Naamad, D. Frydman, E. Broide, A. Zimran, G. Stemer, S. Revel-Vilk   +6 more
doaj   +1 more source

Combination of Flow Cytometry and Functional Imaging for Monitoring of Residual Disease in Myeloma

Leukemia, 2018
The iliac crest is the sampling site for minimal residual disease (MRD) monitoring in multiple myeloma (MM). However, the disease distribution is often heterogeneous, and imaging can be used to complement MRD detection at a single site.
L. Rasche, D. Alapat, M. Kumar, G. Gershner, J. McDonald, C. Wardell, R. Samant, R. V. Van Hemert, J. Epstein, A. Williams, S. Thanendrarajan, C. Schinke, M. Bauer, C. Ashby, R. Tytarenko, F. van Rhee, B. Walker, M. Zangari, B. Barlogie, F. Davies, G. Morgan, N. Weinhold   +21 more
semanticscholar   +1 more source

Down‐regulation of Shh in the hair follicles of mice during chemotherapy‐induced hair loss is mediated by the JAK/STAT1 signaling pathway

FEBS Open Bio, EarlyView.
We found that during chemotherapy‐induced alopecia (CIA), Sonic hedgehog (Shh) expression significantly decreased in hair follicle Shh+ cells, whereas the Janus‐activated kinase/signal transducer and activator of transcription 1 (JAK/STAT1) signaling pathway was markedly activated.
Ruifang Fan, Jingling Huang, Xiang Lin, Tingjiao Lan, Yuli Tang, Ling Sun, Guixuan Zhou   +6 more
wiley   +1 more source

Relevance of Antibody Validation for Flow Cytometry

Cytometry Part A, 2019
Antibody reagents are the key components of multiparametric flow cytometry analysis. Their quality performance is an absolute requirement for reproducible flow cytometry experiments.
T. Kalina, K. Lundsten, P. Engel
semanticscholar   +1 more source

SIRT4 positively regulates autophagy via ULK1, but independently of HDAC6 and OPA1

FEBS Open Bio, EarlyView.
Cells expressing SIRT4 (H161Y), a catalytically inactive mutant of the sirtuin SIRT4, fail to upregulate LC3B‐II and exhibit a reduced autophagic flux under stress conditions. Interestingly, SIRT4(H161Y) promotes phosphorylation of ULK1 at S638 and S758 that are associated with inhibition of autophagy initiation.
Isabell Lehmkuhl, Khawar Amin, Lydia Gabriel, Nils Hampel, Afshin Iram, Julia Hesse, Constanze Wiek, Jasmin Thuy Vy Nguyen, Helmut Hanenberg, Jürgen Scheller, M. Reza Ahmadian, Björn Stork, Doreen M. Floss, Roland P. Piekorz   +13 more
wiley   +1 more source

Engineering tandem VHHs to target different epitopes to enhance antibody‐dependent cell‐mediated cytotoxicity

FEBS Open Bio, EarlyView.
Tandem VHH targeting distinct EGFR epitopes were engineered into a monovalent bispecific antibody (7D12‐EGA1‐Fc) with more potent ADCC without increasing affinity to EGFR. Structural modeling of 7D12‐EGA1‐Fc showed cross‐linking of separate EGFR domains to enhance CD16a engagement on NK cells.
Yuqiang Xu, Hao Jiang, Limin Chen, Fulai Zhou, Ying Jin, Mark L. Chiu   +5 more
wiley   +1 more source