Results 101 to 110 of about 3,125,910 (203)

Engineered Nanoplatforms Targeting Activated Hepatic Stellate Cells for Liver Fibrosis Therapy via TGF‐β Axis Inhibition and Ferroptosis Induction

Advanced Healthcare Materials, EarlyView.
FeS‐VA@lanifibranor (FVL), an activated hepatic stellate cell (aHSC)‐targeted nanoplatform comprising vitamin A‐functionalized two‐dimensional iron sulfide nanosheets and lanifibranor, is engineered for precision therapy of liver fibrosis. FVL suppresses the TGF‐β signaling pathway by inhibiting SMAD2/3 phosphorylation and induces ferroptosis in aHSCs,
Yinuo Yang, Mengyao Mu, Ke Ren, Hui Liu, Pengju Ma, Yingqi Wang, Mengmeng Zhang, Xueli Xu, Wenzhe Li, Wenshuang Gao, Xiao Sun, Qiang Zhu   +11 more
wiley   +1 more source

2‐Deoxyglucose Dendrimer‐Enabled Niclosamide Delivery to FRβ‐Expressing Macrophages Alleviates Endometriosis Progression and Associated Hyperalgesia

Advanced Healthcare Materials, EarlyView.
A folic acid (FA)‐conjugated 2‐deoxyglucose dendrimer selectively targets FRβ+ macrophages in endometriotic lesions, enabling precise intracellular delivery of niclosamide. This nonhormonal nanomedicine enhances drug solubility, provides controlled intracellular release, suppresses lesion growth, reduces inflammation and pain, and establishes FRβ ...
Anubhav Dhull, Mingxin Shi, Madeleine E. Harvey, Taylor M. Page, Rishi Sharma, Kenneth John Goody, Aqib Iqbal Dar, Nash S. Moawad, Vijay Kumar Sirohi, Paul S. Cooke, Kanako Hayashi, Anjali Sharma   +11 more
wiley   +1 more source

Flow cytometry protocol for GLUT4-myc detection on cell surfaces

Insulin and muscle contraction trigger GLUT4 translocation to the plasma membrane, which increases glucose uptake by muscle cells. Insulin resistance and type 2 diabetes are the result of impaired GLUT4 translocation.
Pavarotti, Martin Alejandro, Mayorga, Luis Segundo, Zanni Ruiz, Emilia   +2 more
core   +1 more source

Evaluating the utility of a nanoscale flow cytometer for detection of surface proteins on HIV and extracellular vesicles

Virology Journal
Background Flow virometry (FV) – the application of flow cytometry to viruses – has historically been hindered by the inability of cytometers to detect particles below ~ 300 nm in size.
Jonathan Burnie, Caroline Ouano, Vanessa Costa, Irene Castrosin, Catherine Hammond, Hannah Matthews, John Tigges, Kizzmekia S. Corbett-Helaire   +7 more
doaj   +1 more source

PLL‐g‐HPA Hydrogel Microneedles Loaded With Rhoifolin Target Macrophages to Alleviate Fibroblast Senescence for Diabetic Wound Healing

Advanced Healthcare Materials, EarlyView.
Diabetic wound healing is hindered by a pathological crosstalk wherein elevated IL‐11 induces M1 macrophage polarization, leading to IL‐1β/IL‐6 release and subsequent fibroblast senescence via RAS/p38MAPK/p53/E2F1 signaling. Here, we develop a Gel@M‐Rho microneedle patch that delivers rhoifolin‐loaded, macrophage membrane‐coated nanoparticles to target
Jiewen Liao, Lin Gan, Li Lu, Lizhi Ouyang, Yuan Xiong, Fawwaz Al‐Smadi, Guandong Dai, Mohammad‐Ali Shahbazi, Bobin Mi, Mengfei Liu, Guohui Liu   +10 more
wiley   +1 more source

A new method for detecting mycoplasma in cell cultures using colocalization of DNA and membrane staining

Biochemistry and Biophysics Reports
Mycoplasma often surreptitiously contaminates cell cultures without detection, mainly parasitizing the cell surface and thus interfering to some extent with the cells.
Mengyuan Li, Shan Liu, Cheng Zhou, Mengsi Sun, Yanjie Wang   +4 more
doaj   +1 more source

Water‐Activated Phospholipid Phase‐Separation Gel as an Immunomodulatory Anti‐Adhesion Barrier for Postoperative Adhesion Prevention

Advanced Healthcare Materials, EarlyView.
A water‐activated phospholipid phase‐separation gel is repurposed as a drug‐free surgical barrier for postoperative adhesion prevention. Propylene glycol promotes rapid lamellar gel reinforcement, prolonged local retention, and tissue coverage, while phosphatidylcholine‐mediated immunomodulation suppresses inflammatory and fibrotic responses. This dual‐
Jong‐Ju Lee, Yulseung Sung, Yeji Kim, Heesang Yang, Suyeon Pyo, Byeol Yun, Sung‐Joo Hwang   +6 more
wiley   +1 more source

CD25+CD27+CD70− alloantigen-specific Tregs: promising stable immunotherapy for transplantation

Frontiers in Immunology
The clinical translation of alloantigen-specific regulatory T cells (AS-Tregs) is constrained by their low frequency in peripheral blood, limited purity, and functional instability following prolonged ex vivo expansion.
Arimelek Cortés-Hernández, Arimelek Cortés-Hernández, Saúl Arteaga-Cruz, Saúl Arteaga-Cruz, Iyari I. Martínez Iturbe, Iyari I. Martínez Iturbe, Katya Rosas-Cortina, Marco A. Vigil Mora, Marco A. Vigil Mora, Erick Legorreta-Anguiano, Erick Legorreta-Anguiano, Judith E. Reyes Barrientos, Judith E. Reyes Barrientos, Evelyn K. Álvarez-Salazar, Evelyn K. Álvarez-Salazar, Alejandra Cervera, Beatriz E. Sánchez-Hernández, Armando Gamboa Domínguez, Gloria Soldevila, Gloria Soldevila   +19 more
doaj   +1 more source

An Engineered Biomimetic Nanoreactor With Cascade Functions for Targeted and Combinatorial Therapy of Ischemic Stroke

Advanced Healthcare Materials, EarlyView.
Preparation of RVG‐modified erythrocyte membrane‐coated HMPB nanocarriers loaded with L‐arginine and NBP (RM@HPAN) and its neuroprotective mechanism. After intravenous injection, RM@HPAN achieves prolonged circulation, RVG‐29‐mediated BBB penetration, and ischemic accumulation.
Shaofa Li, Ying Wu, Huimin Wei, Jinyong Teng, Zaizhi Yang, Jufang Chen, Bao Liao, Xiaoqin Huang, Lanqing Meng, Tong Li   +9 more
wiley   +1 more source

Comprehensive In Vitro Profiling Demonstrates Successful Immune Evasion of Bioengineered Immune‐Shielded Heart Valves

Advanced Healthcare Materials, EarlyView.
Living donor heart valves offer superior durability for pediatric patients but trigger immune rejection. Lentiviral delivery of viral immune‐evasion genes US2 and Serpin b9 into donor valve cells and valve tissue suppresses HLA expression, reduces PBMC clustering and cytotoxic killing, and induces transcriptional immune‐tolerance programs, paving the ...
Abraham van Wijk, Leanne Van den Berg, Isa B. M. Velraeds, Valentina Osorio Zuluaga, Emma T. M. Peereboom, Martijn J. W. E. Rabelink, Arnaud Zaldumbide, Rob C. Hoeben, Marie‐Jose T. H. Goumans, Eric Spierings, Carlijn V. C. Bouten, Marijn C. Peters   +11 more
wiley   +1 more source