Results 121 to 130 of about 5,462,613 (380)
EMT‐associated bias in the Parsortix® system observed with pancreatic cancer cell lines
Molecular Oncology, EarlyView.The Parsortix® system was tested for CTC enrichment using pancreatic cancer cell lines with different EMT phenotypes. Spike‐in experiments showed lower recovery of mesenchymal‐like cells. This was confirmed with an EMT‐inducible breast cancer cell line.
Nele Vandenbussche +8 more
wiley +1 more source
Spectral flow cytometry—Quo vadimus?
Cytometry Part A, 2019 FOR 40 years, flow cytometry has been dominated by polychromatic flow cytometry. Despite the fact that Fulwyler’s instrument (1) was based on Coulter volume, fluorescence became the dominant technology within a very few years.
J. Paul Robinson
semanticscholar +1 more source
Automating flow cytometry [PDF]
Cytometry Part A, 2011 FLOW cytometry data analysis has been traditionally based on the identification of cell populations by using strategies founded on the definition of gates in bi-dimensional plots, where an experienced operator selects the subpopulation(s) of interest.
openaire +3 more sources
Molecular Oncology, EarlyView.Non‐small cell lung cancer targeted treatment is limited to a few known genetic alterations, with few alternatives in advanced treatment lines. To direct treatment decisions by drug sensitivity testing (DST), this study compared several methods for tumor cell isolation from malignant effusions, pointing to repeated CD45+ cell depletion for effective ...
Navit Mooshayef +10 more
wiley +1 more source
A quick, reliable, and automated method for fat cell sizing
Journal of Lipid Research, 2003 Mean diameters of fat cells from abdominal tissues from 31 volunteers were determined by three methods based on fat cell isolation after collagenase digestion and methylene blue staining.
Yourka D. Tchoukalova +4 more
doaj
Molecular Oncology, EarlyView.Human cytomegalovirus infection is common in normal prostate epithelium, prostate tumor tissue, and prostate cancer cell lines. CMV promotes cell survival, proliferation, and androgen receptor signaling. Anti‐CMV pharmaceutical compounds in clinical use inhibited cell expansion in prostate cancer models in vitro and in vivo, motivating investigation ...
Johanna Classon +13 more
wiley +1 more source
Data Quality Assessment of Ungated Flow Cytometry Data in High [PDF]
, 2007 Background: The recent development of semi-automated techniques for staining and analyzing flow cytometry samples has presented new challenges. Quality control and quality assessment are critical when developing new high throughput technologies and their
Brinkman, Ryan R +5 more
core +1 more source
Molecular Oncology, EarlyView.Carcinoma‐associated fibroblasts (CAFs) in tumors influence cancer progression. We identified endoglin (ENG) as a key factor in TGF‐β signaling in myofibroblastic CAFs (myCAFs), linked to poor breast cancer outcomes. Inhibiting ENG on myCAFs suppressed the TGF‐β‐Smad2/3 pathway, reducing primary tumor growth and metastasis.
Shoki Okubo +11 more
wiley +1 more source
Case Reports in Medicine, 2011 Aggressive lymphomas can present with symptoms mimicking life-threatening infection. Flow cytometry (FC) is usually recommended for the classification and staging of lymphomas in patients with organomegaly and atypical cells in effusions and blood, after
Nikolaos J. Tsagarakis +13 more
doaj +1 more source