Results 61 to 70 of about 488,726 (317)

An approach of flow velocity measurement on microfluidic chip by near-field scanning microwave microscopy

AIP Advances, 2023
A novel approach for flow velocity measurements on a microfluidic chip using near-field scanning microwave microscopy (NSMM) is proposed. It does not require the tested sample to contain visible particles, as the intrinsic electromagnetic properties of ...
Zhiwei Yuan, Han Luo, Yujie Chen, Xiangyue Yang, Yue Wu, Zhe Wu, Jianlong Liu, Yubin Gong, Baoqing Zeng   +8 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Complex of protection structures on the Shakh River in the Krasnodar Territory

Мелиорация и гидротехника
Purpose: to substantiate a set of measures to ensure reliable protection of the river bank and structures from the impact of water flow and floating objects. Materials and methods. A set of measures to ensure protection of the river bank and structures
Alexander M. Anokhin, Alexander Yu. Garbuz   +1 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

A flowmeter for measuring the dispersed phase velocity in multiphase flow with non-uniform velocity profile

, 2008
This study aims to introduce a new technique to measure the velocity distribution of the dispersed component of a vertical, upward, water continuous two-phase pipe flow. Here, it is proposed that measurements of the variation in the local conductance
Al-Hinai, Sulaiyam, Lucas, Gary
core  

Measurement of Flow Characteristics in a Bubbling Fluidized Bed Using Electrostatic Sensor Arrays [PDF]

, 2016
Fluidized beds are widely applied in a range of industrial processes. In order to maintain the efficient operation of a fluidized bed, the flow parameters in the bed should be monitored continuously. In this paper, electrostatic sensor arrays are used to
Yang, Yongrong, Zhang, Wenbiao, Wang, Jingdai, Yan, Yong   +3 more
core   +1 more source

Assessment of Chicken Fecal Contamination Using Microbial Source Tracking (MST) and Environmental DNA (eDNA) Profiling in Silway River, Philippines

Journal of Xenobiotics
The Silway River has historically failed to meet safe fecal coliform levels due to improper waste disposal. The river mouth is located in General Santos City, the tuna capital of the Philippines and a leading producer of hogs, cattle, and poultry.
Lonny Mar Opog, Joan Cecilia Casila, Rubenito Lampayan, Marisa Sobremisana, Abriel Bulasag, Katsuhide Yokoyama, Soufiane Haddout   +6 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source