Results 81 to 90 of about 7,809,143 (340)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Assessment of Chicken Fecal Contamination Using Microbial Source Tracking (MST) and Environmental DNA (eDNA) Profiling in Silway River, Philippines

Journal of Xenobiotics
The Silway River has historically failed to meet safe fecal coliform levels due to improper waste disposal. The river mouth is located in General Santos City, the tuna capital of the Philippines and a leading producer of hogs, cattle, and poultry.
Lonny Mar Opog, Joan Cecilia Casila, Rubenito Lampayan, Marisa Sobremisana, Abriel Bulasag, Katsuhide Yokoyama, Soufiane Haddout   +6 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimization of Heat Transfer and Flow Performance of Microchannel Liquid-Cooled Plate Based on Orthogonal Test

Buildings
Microchannel liquid-cooled plates are widely used in high-performance electronic devices, but their heat transfer performance and pressure drop characteristics face complex challenges in the design process.
Zhengchao Yang, Qiufei Yao, Yu Wang, Junlong Gu, Zhichen Yu, Qipeng Li, Xiaoyi Sun, Xuejing Yang   +7 more
doaj   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Experimental investigation of transitional flow in a toroidal pipe

, 2013
The flow instability and further transition to turbulence in a toroidal pipe (torus) with curvature (tube-to-coiling diameter) 0.049 is investigated experimentally.
Hof, B., Holzner, M., Kuhlmann, H., Kühnen, J.   +3 more
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Analysis of characteristics of coal fine production and its influence factors in Baode block

Youqicang pingjia yu kaifa, 2023
The issue of coal fine production is increasingly prominent in the development of coal-bed methane. Implementing appropriate measures to control the migration and production of coal fines is crucial for achieving stable and high production of coal-bed ...
MENG Wenhui, ZHANG Wen, WANG Boyang, HAO Shuai, WANG Zebin, PAN Wujie   +5 more
doaj  

Protostellar Jets Enclosed by Low-velocity Outflows

, 2014
A protostellar jet and outflow are calculated for \sim 270 yr following the protostar formation using a three dimensional magnetohydrodynamics simulation, in which both the protostar and its parent cloud are spatially resolved. A high-velocity (\sim100km/
Machida, Masahiro N.
core   +1 more source