Results 61 to 70 of about 55,131 (262)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Why human connection is the true metric of research success

FEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan, Mattias Wei Ren Kon, Xavier Coumoul, Fun Man Fung   +3 more
wiley   +1 more source

Vibration of Shaft System in the Mixed-Flow Pump Induced by the Rotor-Stator Interaction under Partial Load Conditions

Shock and Vibration, 2018
In order to reveal the relationship between rotor-stator interaction-induced unsteady flow and the shaft vibration of the mixed-flow pump, PIV (particle image velocimetry) and axis orbit experiments were carried out synchronously in a mixed-flow pump ...
Wei Li, Leilei Ji, Weidong Shi, Yulu Wang, Ling Zhou, Xiaoping Jiang   +5 more
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Development of a computational framework for low-Reynolds number propeller aeroacoustics

Archives of Mechanics
The paper focuses on the development and validation of a new computational framework designed for the prediction of tonal and broadband noise radiation of propellers of unmanned aerial vehicles (UAVs) operating in the low-Reynolds number regime.
O. Szulc, T. Suresh, P. Flaszyński
doaj   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

A Study on Energy Loss and Transient Flow Characteristics of a Large Volute Centrifugal Pump During Power-Off Process Under Cavitation Conditions

Journal of Marine Science and Engineering
A novel pumped storage system using centrifugal pumps to transfer water between reservoirs in coastal hydropower plants has significantly mitigated grid instability. However, frequent start–stop operations of large vertical centrifugal pumps, which serve
Qingzhao Pang, Desheng Zhang, Gang Yang, Xi Shen, Qiang Pan, Linlin Geng, Qinghui Lu   +6 more
doaj   +1 more source

Design and Optimization of Fluid Machinery

Water
Fluid machinery plays an indispensable role in fundamental human activities and is widely used in areas such as desulfurization in coal-fired power plants, power generation in hydropower stations, water transmission, and agricultural irrigation. Due to the expanding range of applications, there are increasingly higher demands for the performance of ...
Leilei Ji, Ramesh K. Agarwal, Yang Yang
openaire   +1 more source