Results 1 to 10 of about 3,434,430 (303)

Heterogeneous transition metal–based fluorescence polarization (HTFP) assay for probing protein interactions

BioTechniques, 2009
Analyses of protein interactions are fundamental for the investigation of molecular mechanisms responsible for cellular processes and diseases, as well as for drug discovery in the pharmaceutical industry.
Alexander Riechers, Jennifer Schmidt, Burkhard König, Anja Katrin Bosserhoff   +3 more
doaj   +2 more sources

Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence [PDF]

Biosensors, 2013
We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude.
Stefan Seeger, Thomas Ruckstuhl, Christian M. Winterflood   +2 more
doaj   +2 more sources

Insight into the binding of a non-toxic, self-assembling aromatic tripeptide with ct-DNA: Spectroscopic and viscositic studies

Biochemistry and Biophysics Reports, 2017
The report describes the synthesis, self-association and DNA binding studies of an aromatic tripeptide H-Phe-Phe-Phe-OH (FFF). The peptide backbone adopts β—sheet conformation both in solid and solution.
Soumi Biswas, Satyabrata Samui, Arpita Chakraborty, Sagar Biswas, Debapriya De, Utpal Ghosh, Apurba K. Das, Jishu Naskar   +7 more
doaj   +1 more source

Tryptophan Fluorescence Quenching Assays for Measuring Protein-ligand Binding Affinities: Principles and a Practical Guide

Bio-Protocol, 2019
Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a protein that results from changes in the local ...
Anthony Yammine, Jinlong Gao, Ann Kwan
doaj   +1 more source

Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma

Sensors, 2009
Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds.
Kiyotaka Sakai, Takehito Ogawa, Takehiro Miyasaka, Satoka Aoyagi   +3 more
doaj   +1 more source

Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence

Biochemistry and Biophysics Reports, 2019
While the binding of biotin by streptavidin does not appear to be cooperative in the traditional sense of altered binding strength, it has been suggested that it may be cooperative in terms of differential structural changes in the protein.
Mark J. Waner, James M. Hiznay, Anthony T. Mustovich, William Patton, Charles Ponyik, David P. Mascotti   +5 more
doaj   +1 more source

Establishment of a graphene quantum dot (GQD) based steroid binding assay for the nuclear progesterone receptor (pgr)

Biochemistry and Biophysics Reports
Previously, we established a homogeneous assay for membrane progesterone receptor alpha (mPRα) ligands by conjugating semiconductor nanoparticles known as graphene quantum dots (GQDs) to mPRα. When mixed with a progesterone-BSA-fluorescein isothiocyanate
Md. Forhad Hossain, Shakhawat Hossain, Md. Maisum Sarwar Jyoti, Yuki Omori, Saokat Ahamed, Toshinobu Tokumoto   +5 more
doaj   +1 more source

Characterization of MaltOBP1, a Minus-C Odorant-Binding Protein, From the Japanese Pine Sawyer Beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae)

Frontiers in Physiology, 2020
Insect Odorant-Binding Proteins (OBPs) play crucial roles in the discrimination, binding and transportation of odorants. Herein, the full-length cDNA sequence of Minus-C OBP1 (MaltOBP1) from the Japanese pine sawyer beetle, Monochamus alternatus, was ...
Fangmei Zhang, Fangmei Zhang, Austin Merchant, Zhibin Zhao, Yunhui Zhang, Jing Zhang, Qingwen Zhang, Qinghua Wang, Xuguo Zhou, Xiangrui Li, Xiangrui Li   +10 more
doaj   +1 more source

A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments

Cell Reports: Methods, 2023
Summary: Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. In this study, we employed an in vitro single-molecule DNA flow-stretching assay
Miranda Molina, Lindsey E. Way, Zhongqing Ren, Qin Liao, Bianca Guerra, Brandon Shields, Xindan Wang, HyeongJun Kim   +7 more
doaj   +1 more source

Enhancing Target Detection: A Fluorescence-Based Streptavidin-Bead Displacement Assay

Biosensors
Fluorescence-based aptasensors have been regarded as innovative analytical tools for the detection and quantification of analytes in many fields, including medicine and therapeutics. Using DNA aptamers as the biosensor recognition component, conventional
Sireethorn Tungsirisurp, Nunzianda Frascione   +1 more
doaj   +1 more source