Results 1 to 10 of about 3,257,031 (299)
A Simple Theoretical Model for Fluorescence Polarization Binding Assay Development [PDF]
Journal of Biomolecular Screening, 2006 Fluorescence polarization is a screening technology that is radioactivity free, homogeneous, and ratiometric. The signal measured with this technology is a weighted value of free and bound ligand. As a consequence, saturation curves are accessible only after calculation of the corresponding concentrations of free and bound ligand.
Jean Albert Boutin
exaly +3 more sources
Development of a competitive fluorescence-based synaptosome binding assay for brevetoxins [PDF]
Harmful Algae, 2012 Brevetoxins are a family of ladder-frame polyether toxins produced during blooms of the marine dinoflagellate Karenia brevis. Inhalation of brevetoxins aerosolized by wind and wave action can lead to asthma-like symptoms in beach goers. Consumption of either shellfish or finfish exposed to K.
Andrea J Bourdelais +2 more
exaly +3 more sources
Biomolecules, 2019 Neuromelanin, the polymeric form of dopamine which accumulates in aging neuronal tissue, is increasingly recognized as a functional and critical component of a healthy and active adult human brain.
Jackson Fink +4 more
doaj +3 more sources
A Fluorescence Lifetime–Based Binding Assay to Characterize Kinase Inhibitors [PDF]
SLAS Discovery, 2007 The authors present a fluorescence lifetime-based kinase binding assay that identifies and characterizes compounds that bind to the adenosine triphosphate (ATP)-binding pocket of a range of tyrosine and serine/threonine kinases. The assay is based on displacement of an Alexa Fluor 647 conjugate of staurosporine from the ATP-binding site of a kinase ...
Kurt W Vogel
exaly +3 more sources
Peptidoglycan glycosyltransferase-ligand binding assay based on tryptophan fluorescence quenching [PDF]
Biochimie, 2018 Peptidoglycan glycosyltransferases (GTase) of family 51 are essential enzymes for the synthesis of the glycan chains of the bacterial cell wall. They are considered potential antibacterial target, but discovery of inhibitors was hampered so far by the lack of efficient and affordable screening assay. Here we used Staphylococcus aureus MtgA to introduce
André Matagne +2 more
exaly +3 more sources
Insects, 2021 Olfaction is essential in some behaviors of honeybee, such as nursing, foraging, attracting a mate, social communication, and kin recognition. OBPs (odorant binding proteins) play a key role in the first step of olfactory perception.
Huiting Zhao +4 more
doaj +1 more source
Journal of Integrative Agriculture, 2022 Chemosensory proteins (CSPs) are important molecular components of the insect olfactory system, which are involved in capturing, binding, and transporting hydrophobic odour molecules across the sensillum in sensillar lymph in regulating insect behavior ...
Muhammad Irfan WARIS +5 more
doaj +1 more source
POT1 Stability and Binding Measured by Fluorescence Thermal Shift Assays [PDF]
PLOS ONE, 2021 Abstract The protein POT1 ( P rotection o f T elomeres 1) is an integral part of the shelterin complex that protects the ends of human chromosomes from degradation or end fusions.
Lynn W. DeLeeuw +7 more
openaire +4 more sources
Applying a high-throughput fluorescence polarization assay for the discovery of chemical probes blocking La:RNA interactions in vitro and in cells. [PDF]
PLoS ONE, 2017 The RNA-binding protein La is overexpressed in a number of tumor tissues and is thought to support tumorigenesis by binding to and facilitating the expression of mRNAs encoding tumor-promoting and anti-apoptotic factors.
Gunhild Sommer +5 more
doaj +1 more source
Characterization and Functional Analysis of OcomOBP7 in Ophraella communa Lesage
Insects, 2023 The olfactory system plays a key role in various insect behaviors, and odorant-binding proteins participate in the first step of the olfactory process. Ophraella communa Lesage is an oligophagous phytophagous insect that is a specific biological control ...
Yang Yue +6 more
doaj +1 more source