Results 31 to 40 of about 3,434,430 (303)

Novel Fluorescence Competition Assay for Retinoic Acid Binding Proteins [PDF]

ACS Medicinal Chemistry Letters, 2018
Vitamin A derived retinoid compounds have multiple, powerful roles in the cellular growth and development cycle and, as a result, have attracted significant attention from both academic and pharmaceutical research in developing and characterizing synthetic retinoid analogues.
Charles W. E. Tomlinson, David R. Chisholm, Roy Valentine, Andrew Whiting, Ehmke Pohl   +4 more
openaire   +4 more sources

Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach [PDF]

, 2015
People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University
J. Carlos Penedo, Maria Figueira-gonzalez, White, Malcolm F, Ackermann, Katrin, Katrin Ackermann, Penedo, Carlos, Peregrina, Jose Ramon, Bode, Bela Ernest, Michael J. Morten, Figueira-Gonzalez, Maria, Malcolm F. White, Bela E. Bode, Morten, Michael John, Jose R. Peregrina, Bode, Bela Ernest; id_orcid, White, Malcolm F; id_orcid, Penedo, Carlos; id_orcid, Ackermann, Katrin; id_orcid   +17 more
core   +1 more source

Detection of SARS-CoV 2 receptor binding domain using fluorescence probe and DNA flowers enabled by rolling circle amplification [PDF]

, 2022
Using rolling circle amplification (RCA) and two different ways of signal readout, we developed analytical methods to detect the receptor binding domain (RBD) of SARS-CoV 2 spike protein (S protein). We modified streptavidin-coated magnetic beads with an
Man, Zhang, Lei, Ye
core   +2 more sources

Visualizing helicases unwinding DNA at the single molecule level. [PDF]

, 2010
DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes ...
Fili, Natali, Mashanov, Gregory I, Yeeles, Joseph T P, Toseland, Christopher P, Wallace, Mark I, Webb, Martin R, Batters, Christopher, Dillingham, Mark S, Molloy, Justin E   +8 more
core   +1 more source

Allosteric inhibition of cobalt binding to albumin by fatty acids : implications for the detection of myocardial ischemia [PDF]

, 2012
The biomarker "ischemia-modified albumin" (IMA), measured by the albumin-cobalt-binding assay (ACB assay), is the only FDA-approved biomarker for early diagnosis of myocardial ischemia.
Peter J. Sadler, Sadler, P. J., Sadler, Peter J., Claudia A. Blindauer, Teresa J. T. Pinheiro, Stewart, Alan J., Blindauer, Claudia A., Pinheiro, Teresa J. T., Jin Lu, Lu, Jin, Alan J. Stewart, Stewart, Alan J.; id_orcid   +11 more
core   +1 more source

Screening for oligonucleotide binding affinity by a convenient fluorescence competition assay [PDF]

Nucleic Acids Research, 1999
A competitive homogeneous quenched fluorescence assay system is described for the high throughput screening of DNA conjugates that bind to single-stranded DNA. Fluorescence signal is generated by competitive binding of the sample molecule to a target strand labelled with a quencher probe, which is otherwise hybridised to a complementary strand ...
J G, Harrison, X, Liu, S, Balasubramanian   +2 more
openaire   +3 more sources

Conformational dynamics of the tetracycline-binding aptamer [PDF]

, 2011
The conformational dynamics induced by ligand binding to the tetracycline-binding aptamer is monitored via stopped-flow fluorescence spectroscopy and time-correlated single photon counting experiments.
Weigand, Julia E., Josef Wachtveitl, Suess, Beatrix, Ute Förster, Trojanowski, Peter, Peter Trojanowski, Julia E. Weigand, Förster, Ute, Wachtveitl, Josef, Süß, Beatrix, Beatrix Suess   +10 more
core   +1 more source

Immunofunctional assay of human growth hormone (hGH) in serum: A possible consensus for quantitative hGH measurement [PDF]

, 1996
Confirmation of the diagnosis of GH deficiency in adults and children involves provocative testing for human (h) GH. Different commercially available immunoassays yield largely discrepant results in the measurement of GH levels in human serum.
Strasburger, Christian J., Pflaum, Claus-Dieter, Dressendörfer, Regina A., Wu, Zida   +3 more
core   +1 more source

Fluorescent Receptor Binding Assay for Detecting Ciguatoxins in Fish

PLOS ONE, 2016
Ciguatera fish poisoning is an illness suffered by > 50,000 people yearly after consumption of fish containing ciguatoxins (CTXs). One of the current methodologies to detect ciguatoxins in fish is a radiolabeled receptor binding assay (RBA(R)). However, the license requirements and regulations pertaining to radioisotope utilization can limit the ...
Hardison, D. Ransom, Holland, William C., Mccall, Jennifer R., Bourdelais, Andrea J., Baden, Daniel G., Darius, H. Taiana, Chinain, Mireille, Tester, Patricia A., Shea, Damian, Quintana, Harold A. Flores, Morris, James A., Jr., Litaker, R. Wayne   +11 more
openaire   +5 more sources

Development of a competitive fluorescence-based synaptosome binding assay for brevetoxins [PDF]

Harmful Algae, 2012
Brevetoxins are a family of ladder-frame polyether toxins produced during blooms of the marine dinoflagellate Karenia brevis. Inhalation of brevetoxins aerosolized by wind and wave action can lead to asthma-like symptoms in beach goers. Consumption of either shellfish or finfish exposed to K.
Jennifer R, McCall, Henry M, Jacocks, Daniel G, Baden, Andrea J, Bourdelais   +3 more
openaire   +2 more sources