Bio-Protocol, 2019 Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a protein that results from changes in the local ...
Anthony Yammine, Jinlong Gao, Ann Kwan
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Immunofunctional assay of human growth hormone (hGH) in serum: A possible consensus for quantitative hGH measurement [PDF]
, 1996 Confirmation of the diagnosis of GH deficiency in adults and children involves provocative testing for human (h) GH. Different commercially available immunoassays yield largely discrepant results in the measurement of GH levels in human serum.
Strasburger, Christian J. +3 more
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Translational and rotational motions of albumin sensed by a non-covalent associated porphyrin under physiological and acidic conditions: a fluorescence correlation spectroscopy and time resolved anisotropy study. [PDF]
, 2008 The interaction between a free-base, anionic water-soluble porphyrin, TSPP, and the drug carrier protein, bovine serum albumin (BSA) has been studied by time-resolved fluorescence anisotropy (TRFA) and fluorescence correlation spectroscopy (FCS) at two ...
Costa, S.M. +4 more
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Sensors, 2009 Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds.
Kiyotaka Sakai +3 more
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Allosteric inhibition of cobalt binding to albumin by fatty acids : implications for the detection of myocardial ischemia [PDF]
, 2012 The biomarker "ischemia-modified albumin" (IMA), measured by the albumin-cobalt-binding assay (ACB assay), is the only FDA-approved biomarker for early diagnosis of myocardial ischemia.
Peter J. Sadler +10 more
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Visualizing helicases unwinding DNA at the single molecule level. [PDF]
, 2010 DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes ...
Fili, Natali +17 more
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Screening for oligonucleotide binding affinity by a convenient fluorescence competition assay [PDF]
Nucleic Acids Research, 1999 A competitive homogeneous quenched fluorescence assay system is described for the high throughput screening of DNA conjugates that bind to single-stranded DNA. Fluorescence signal is generated by competitive binding of the sample molecule to a target strand labelled with a quencher probe, which is otherwise hybridised to a complementary strand ...
J G, Harrison +2 more
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Synthesis of a Coumarin-Based PPARγ Fluorescence Probe for Competitive Binding Assay [PDF]
International Journal of Molecular Sciences, 2021 Peroxisome proliferator-activated receptor γ (PPARγ) is a molecular target of metabolic syndrome and inflammatory disease. PPARγ is an important nuclear receptor and numerous PPARγ ligands were developed to date; thus, efficient assay methods are important.
Chisato Yoshikawa +3 more
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An albumin-derived peptide scaffold capable of binding and catalysis [PDF]
, 2012 We have identified a 101-amino-acid polypeptide derived from the sequence surrounding the IIA binding site of human albumin. The polypeptide contains residues that make contact with ligands as warfarin in the parent protein, and eight cysteine residues ...
SBLATTERO, DANIELE +30 more
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Biochemistry and Biophysics Reports, 2019 While the binding of biotin by streptavidin does not appear to be cooperative in the traditional sense of altered binding strength, it has been suggested that it may be cooperative in terms of differential structural changes in the protein.
Mark J. Waner +5 more
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