Results 21 to 30 of about 3,434,430 (303)
Frontiers in Molecular Biosciences, 2023 Dopamine receptors are G-protein-coupled receptors that are connected to severe neurological disorders. The development of new ligands targeting these receptors enables gaining a deeper insight into the receptor functioning, including binding mechanisms,
Maris-Johanna Tahk +10 more
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Fluorescent Assay for Estimating the Binding of Erythromycin Derivatives to Ribosomes [PDF]
Antimicrobial Agents and Chemotherapy, 1978 A fluorescent erythromycin derivative was used to determine the binding of erythromycin derivatives to ribosomes. The assay is rapid, sensitive, and convenient. Because measurements are made in solution, they represent equilibrium conditions, unlike filter binding assays which perturb the equilibrium.
P, Brandt-Rauf +3 more
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Fluorescence resonance energy transfer studies of protein interactions [PDF]
, 2008 This thesis presents an investigation of fluorescence resonance energy transfer (FRET) as a reporting signal for protein-protein interactions. Quantitative optical assays to measure protein binding, conjugation and deconjugation are developed and results
Martin, Sarah Friede
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Foods, 2022 In this study, the identity of our recently produced natural TetR protein was identified by using the LC-ESI-MS/MS technique, and its recognition mechanisms, including the binding pocket, contact amino acids, intermolecular forces, binding sites, binding
Wanqiu Xia, Jing Liu, Jianping Wang
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High-throughput fluorescence assay for membrane-protein interaction
Journal of Lipid Research, 2013 Membrane-protein interaction plays key roles in a wide variety of biological processes. Although various methods have been employed to measure membrane binding of soluble proteins, a robust high-throughput assay that is universally applicable to all ...
Hyunjin Kim +2 more
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Assessing the Binding and Endocytosis Activity of Cellular Receptors Using GFP-Ligand Fusions
BioTechniques, 2000 We have developed a simple scheme for characterizing ligand-receptor binding and post-binding activity on living cells. Our approach makes use of green fluorescent protein (GFP) as an auto-fluorescent tag to label protein ligands. We have constructed GFP-
Lali K. Medina-Kauwe +3 more
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Imaging beads-retained prey assay for rapid and quantitative protein-protein interaction. [PDF]
PLoS ONE, 2013 Conventional Western blot based pull-down methods involve lengthy and laborious work and the results are generally not quantitative. Here, we report the imaging beads-retained prey (IBRP) assay that is rapid and quantitative in studying protein-protein ...
Yan Zhou, Wanjin Hong, Lei Lu
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Insects, 2020 The wheat blossom midge Sitodiplosis mosellana, one of the most disastrous wheat pests, depends highly on olfactory cues to track suitable plants. To better understand the olfactory recognition mechanisms involved in host selection, in the present study ...
Weining Cheng +4 more
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AFBI assay – Aptamer Fluorescence Binding and Internalization assay for cultured adherent cells [PDF]
Methods, 2016 The SELEX (Systematic Evolution of Ligands by Exponential Enrichment) process allows for the enrichment of DNA or RNA aptamers from a complex nucleic acid library that are specific for a target molecule. The SELEX process has been adapted from identifying aptamers in vitro using recombinant target protein to cell-based methodologies (Cell-SELEX), where
William H, Thiel, Paloma H, Giangrande
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Time-Resolved Fluorescence Investigation of the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein: Influence of the Binding of Nucleic Acids [PDF]
, 1999 Depending on the HIV-1 isolate, MN or BH10, the nucleocapsid protein, NCp7, corresponds to a 55- or 71-amino acid length product, respectively. The MN NCp7 contains a single Trp residue at position 37 in the distal zinc finger motif, and the BH10 NCp7 ...
Piemont, Etienne +13 more
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