Results 11 to 20 of about 3,257,031 (299)
Development of a quantitative fluorescence-based ligand-binding assay [PDF]
Scientific Reports, 2016 AbstractA major goal of biology is to develop a quantitative ligand-binding assay that does not involve the use of radioactivity. Existing fluorescence-based assays have a serious drawback due to fluorescence quenching that accompanies the binding of fluorescently-labeled ligands to their receptors. This limitation of existing fluorescence-based assays
Breen, Conor +2 more
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BioTechniques, 2009 Analyses of protein interactions are fundamental for the investigation of molecular mechanisms responsible for cellular processes and diseases, as well as for drug discovery in the pharmaceutical industry.
Alexander Riechers +3 more
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Azadioxatriangulenium: a long fluorescence lifetime fluorophore for large biomolecule binding assay [PDF]
Methods and Applications in Fluorescence, 2013 Of the many optical bioassays available, sensing by fluorescence anisotropy has great advantages as it provides a sensitive, instrumentally simple, ratiometric method of detection. However, it is hampered by a severe limitation, as the emission lifetime of the label needs to be comparable to the correlation lifetime (tumbling time) of the biomolecule ...
Sørensen, Thomas Just +6 more
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New approach in multipurpose optical diagnostics: fluorescence based assay for simultaneous determination of physicochemical parameters [PDF]
, 2009 The development of sensors assays for comprehensive characterisation of biological samples and effective minimal-invasive diagnostics is highly prioritised. Last decade this research area has been actively developing due to possibility of simultaneous,
Moczko, Ewa
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Frontiers in Molecular Biosciences, 2023 Dopamine receptors are G-protein-coupled receptors that are connected to severe neurological disorders. The development of new ligands targeting these receptors enables gaining a deeper insight into the receptor functioning, including binding mechanisms,
Maris-Johanna Tahk +10 more
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Fluorescent Assay for Estimating the Binding of Erythromycin Derivatives to Ribosomes [PDF]
Antimicrobial Agents and Chemotherapy, 1978 A fluorescent erythromycin derivative was used to determine the binding of erythromycin derivatives to ribosomes. The assay is rapid, sensitive, and convenient. Because measurements are made in solution, they represent equilibrium conditions, unlike filter binding assays which perturb the equilibrium.
P, Brandt-Rauf +3 more
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Fluorescence resonance energy transfer studies of protein interactions [PDF]
, 2008 This thesis presents an investigation of fluorescence resonance energy transfer (FRET) as a reporting signal for protein-protein interactions. Quantitative optical assays to measure protein binding, conjugation and deconjugation are developed and results
Martin, Sarah Friede
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Foods, 2022 In this study, the identity of our recently produced natural TetR protein was identified by using the LC-ESI-MS/MS technique, and its recognition mechanisms, including the binding pocket, contact amino acids, intermolecular forces, binding sites, binding
Wanqiu Xia, Jing Liu, Jianping Wang
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High-throughput fluorescence assay for membrane-protein interaction
Journal of Lipid Research, 2013 Membrane-protein interaction plays key roles in a wide variety of biological processes. Although various methods have been employed to measure membrane binding of soluble proteins, a robust high-throughput assay that is universally applicable to all ...
Hyunjin Kim +2 more
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Assessing the Binding and Endocytosis Activity of Cellular Receptors Using GFP-Ligand Fusions
BioTechniques, 2000 We have developed a simple scheme for characterizing ligand-receptor binding and post-binding activity on living cells. Our approach makes use of green fluorescent protein (GFP) as an auto-fluorescent tag to label protein ligands. We have constructed GFP-
Lali K. Medina-Kauwe +3 more
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