Results 11 to 20 of about 3,434,430 (303)
Development of a quantitative fluorescence-based ligand-binding assay [PDF]
Scientific Reports, 2016 AbstractA major goal of biology is to develop a quantitative ligand-binding assay that does not involve the use of radioactivity. Existing fluorescence-based assays have a serious drawback due to fluorescence quenching that accompanies the binding of fluorescently-labeled ligands to their receptors. This limitation of existing fluorescence-based assays
Breen, Conor +2 more
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Synthesis of a Coumarin-Based PPARγ Fluorescence Probe for Competitive Binding Assay [PDF]
International Journal of Molecular Sciences, 2021 Peroxisome proliferator-activated receptor γ (PPARγ) is a molecular target of metabolic syndrome and inflammatory disease. PPARγ is an important nuclear receptor and numerous PPARγ ligands were developed to date; thus, efficient assay methods are important.
Chisato Yoshikawa +3 more
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Azadioxatriangulenium: a long fluorescence lifetime fluorophore for large biomolecule binding assay [PDF]
Methods and Applications in Fluorescence, 2013 Of the many optical bioassays available, sensing by fluorescence anisotropy has great advantages as it provides a sensitive, instrumentally simple, ratiometric method of detection. However, it is hampered by a severe limitation, as the emission lifetime of the label needs to be comparable to the correlation lifetime (tumbling time) of the biomolecule ...
Sørensen, Thomas Just +6 more
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Peptidoglycan glycosyltransferase-ligand binding assay based on tryptophan fluorescence quenching [PDF]
Biochimie, 2018 Peptidoglycan glycosyltransferases (GTase) of family 51 are essential enzymes for the synthesis of the glycan chains of the bacterial cell wall. They are considered potential antibacterial target, but discovery of inhibitors was hampered so far by the lack of efficient and affordable screening assay. Here we used Staphylococcus aureus MtgA to introduce
Dahmane, Ismahene +5 more
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Scientific ReportsTelomerase reverse transcriptase is crucial for cellular development, regeneration, and disease processes. Strategies for both telomerase activation and inhibition have been intensively explored in the past decades.
Jia Li Ye +6 more
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Insects, 2021 Olfaction is essential in some behaviors of honeybee, such as nursing, foraging, attracting a mate, social communication, and kin recognition. OBPs (odorant binding proteins) play a key role in the first step of olfactory perception.
Huiting Zhao +4 more
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Journal of Integrative Agriculture, 2022 Chemosensory proteins (CSPs) are important molecular components of the insect olfactory system, which are involved in capturing, binding, and transporting hydrophobic odour molecules across the sensillum in sensillar lymph in regulating insect behavior ...
Muhammad Irfan WARIS +5 more
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POT1 Stability and Binding Measured by Fluorescence Thermal Shift Assays [PDF]
PLOS ONE, 2021 Abstract The protein POT1 ( P rotection o f T elomeres 1) is an integral part of the shelterin complex that protects the ends of human chromosomes from degradation or end fusions.
Lynn W. DeLeeuw +7 more
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Applying a high-throughput fluorescence polarization assay for the discovery of chemical probes blocking La:RNA interactions in vitro and in cells. [PDF]
PLoS ONE, 2017 The RNA-binding protein La is overexpressed in a number of tumor tissues and is thought to support tumorigenesis by binding to and facilitating the expression of mRNAs encoding tumor-promoting and anti-apoptotic factors.
Gunhild Sommer +5 more
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Characterization and Functional Analysis of OcomOBP7 in Ophraella communa Lesage
Insects, 2023 The olfactory system plays a key role in various insect behaviors, and odorant-binding proteins participate in the first step of the olfactory process. Ophraella communa Lesage is an oligophagous phytophagous insect that is a specific biological control ...
Yang Yue +6 more
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