Results 51 to 60 of about 3,434,430 (303)

Development of a Microplate-Based, Electrophoretic Fluorescent Protein Kinase A Assay: Comparison with Filter-Binding and Fluorescence Polarization Assay Formats [PDF]

SLAS Discovery, 2005
A microplate-based electrophoretic assay has been developed for the serine/threonine kinase protein kinase A (PKA). The ElectroCapture PKA assay developed uses a positively charged, lissamine-rhodamine-labeled kemptide peptide substrate for the kinase reaction and Nanogen's ElectroCapture HTS Workstation and 384-well laminated membrane plates to ...
Siobhan M, Miick, Shila, Jalali, Brian P, Dwyer, John, Havens, Donald, Thomas, Manuel A, Jimenez, Mathew T, Simpson, Betsy, Zile, Karen L, Huss, Robert M, Campbell   +9 more
openaire   +2 more sources

Plasma L-ergothioneine measurement by high-performance liquid chromatography and capillary electrophoresis after a pre-column derivatization with 5-iodoacetamidofluorescein (5-IAF) and fluorescence detection [PDF]

, 2013
Two sensitive and reproducible capillary electrophoresis and high-performance liquid chromatography-fluorescence procedures were established for quantitative determination of L-egothioneine in plasma.
Pintus, Gianfranco, Angelo Zinellu, Erre, Gian Luca, Carru, Ciriaco, Salvatore Sotgia, Ciriaco Carru, Sotgia, Salvatore, Erre GL, Gianfranco Pintus, Zinellu, Angelo, Deiana, Luca, Gerard Aime Pinna, Luca Deiana, Pisanu, Elisabetta, Elisabetta Pisanu, Gian Luca Erre, Pinna, Gérard Aimé, Sotgia S, Pisanu Elisabetta, Zinellu Angelo, Deiana Luca, Sotgia Salvatore, Pinna Gerard Aime, Erre Gian Luca, Pintus Gianfranco, Carru Ciriaco   +25 more
core   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Synthesis and characterization of a functional benzoylecgonine – bovine serum albumin conjugate for quantification of a humanized monoclonal anti-cocaine antibody

Biochemistry and Biophysics Reports
We have developed a humanized monoclonal anti-cocaine antibody (h2E2), as a potential solution for cocaine use disorder. A key milestone was the development of an assay to quantify this monoclonal antibody (mAb) in animal and human blood.
Rose P. Webster, Terence L. Kirley, Andrew R. Ray, Zhen-Hin Chan, Andrew B. Norman   +4 more
doaj   +1 more source

Structure, Binding Characteristics, and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm, Helicoverpa armigera (Hübner)

Journal of Integrative Agriculture, 2012
The full-length sequence of the odorant binding protein 5 gene, HarmOBP5, was obtained from an antennae cDNA library of cotton bollworm, Helicoverpa armigera (Hübner). The cDNA contains a 444 bp open reading frame, encoding a protein with 147 amino acids,
Tian-tao ZHANG, Wei-xuan WANG, Shao-hua GU, Zi-ding ZHANG, Kong-ming WU, Yong-jun ZHANG, Yu-yuan GUO   +6 more
doaj   +1 more source

Spatial Expression Analysis of Odorant Binding Proteins in Both Sexes of the Aphid Parasitoid Aphidius gifuensis and Their Ligand Binding Properties

Frontiers in Physiology, 2022
In China, Aphidius gifuensis is one of the most common endoparasitoids of the green peach aphid Myzus persicae and grain aphid Sitobion miscanthi in the field.
Xin Jiang, Xin Jiang, Yaoguo Qin, Yaoguo Qin, Jun Jiang, Yun Xu, Frédéric Francis, Jia Fan, Julian Chen   +8 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

Molecular and Functional Characterization of Odorant Binding Protein 7 From the Oriental Fruit Moth Grapholita molesta (Busck) (Lepidoptera: Tortricidae)

Frontiers in Physiology, 2018
Odorant-binding proteins (OBPs) are widely and abundantly distributed in the insect sensillar lymph and are essential for insect olfactory processes. The OBPs can capture and transfer odor molecules across the sensillum lymph to odorant receptors and ...
Xiu-Lin Chen, Xiu-Lin Chen, Guang-Wei Li, Xiang-Li Xu, Jun-Xiang Wu   +4 more
doaj   +1 more source

Characterization of the chemosensory protein EforCSP3 and its potential involvement in host location by Encarsia formosa

Journal of Integrative Agriculture, 2023
Chemosensory proteins (CSPs) perform several functions in insects. This study performed the gene expression, ligand-binding, and molecular docking assays on the EforCSP3 identified in the parasitoid wasp Encarsia formosa, to determine whether EforCSP3 ...
Ke WANG, Yan-yan HE, You-jun ZHANG, Zhao-jiang GUO, Wen XIE, Qing-jun WU, Shao-li WANG   +6 more
doaj   +1 more source

A Fluorescence-Based Assay to Determine PDZ–Ligand Binding Thermodynamics

, 2021
Postsynaptic density-95, disks-large, and zonula occludens-1 (PDZ) domain interactions with cognate linear binding motifs (i.e., PDZ-binding motifs or PBMs) are important for many biological processes and can be pathological when disrupted. There are hundreds of PDZ-PBM interactions reported but few have been quantitatively determined.
Young Joo, Sun, Ernesto J, Fuentes
openaire   +3 more sources