Results 71 to 80 of about 3,434,430 (303)

Mutant p53^R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation. [PDF]

Mol Oncol
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Lim MC, Guo AK, Itahana Y, Kannan S, Goh V, Ong M, Tay TYW, Ye W, Krishnan SR, Nishiyama J, Verma CS, Itahana K.   +11 more
europepmc   +2 more sources

A Simple Fluorescence Affinity Assay to Decipher Uranyl‐Binding to Native Proteins

Angewandte Chemie, 2022
AbstractDetermining the affinity of proteins for uranyl is key to understand the toxicity of this cation and to further develop decorporation strategies. However, usual techniques to achieve that goal often require specific equipment and expertise. Here, we propose a simple, efficient, fluorescence‐based method to assess the affinity of proteins and ...
Laporte, Fanny, Chenavier, Yves, Botz, Alexandra, Gateau, Christelle, Lebrun, Colette, Hostachy, Sarah, Vidaud, Claude, Delangle, Pascale   +7 more
openaire   +4 more sources

Optimization of the fluorescence polarization (FP) assay.

, 2012
A. Fluorescence polarization (mP) of free Bocillin-FL at various concentrations from 0.002 to 4 µM. B. Binding experiments with Bocillin-FL (1 µM) and PBP 2 at various protein concentrations from 0.2 to 4 µM.
Yuri K. Peterson (134563), Shauna M. Swanson (134561), Alena Fedarovich (134557), Robert A. Nicholas (134565), Christopher Davies (67862), Kevin A. Djordjevic (134559)   +5 more
core   +1 more source

Neutralization of the PTX3/CD44 axis suppresses TGF‐β1 signaling in kidney fibrosis

FEBS Letters, EarlyView.
This study investigates how the PTX3/CD44 axis promotes kidney fibrosis by activating TGF‐β1 signaling. We demonstrate that the neutralizing antibody WHC‐001 effectively inhibits this pathway, suppressing myofibroblast activation and partial epithelial–mesenchymal transition (EMT).
Chao‐Chun Cheng, Shen‐Shin Chang, Feng‐Wei Chen, Ying‐Chieh Su, Thanh Phuong Nguyen, Yu‐Wei Hsiao, Jhih‐Ying Chi, Hsin‐Yin Liang, Ju‐Ming Wang   +8 more
wiley   +1 more source

Fluorescence anisotropy microplate assay for analysis of steroid receptor-DNA interactions

BioTechniques, 2004
To analyze the interactions of steroid/nuclear hormone receptors with their DNA response elements, we used ultra low-volume microplates to develop a simple and rapid fluorescence anisotropy assay. The novel fluorescence anisotropy microplate assay (FAMA)
Stanley Y. Wang, Bonnie S. Ahn, Rebecca Harris, Steven K. Nordeen, David J. Shapiro   +4 more
doaj   +1 more source

A high-throughput fluorescence lifetime-based assay to detect binding of myosin-binding protein C to F-actin

, 2021
Binding properties of actin-binding proteins are typically evaluated by cosedimentation assays. However, this method is time-consuming, involves multiple steps, and has a limited throughput. These shortcomings preclude its use in screening for drugs that
Victoria C. Lepak, Bunch, Thomas A., Bortz, Kellan M., Colson, Brett A., Thomas A. Bunch, Kellan M. Bortz, Brett A. Colson, Lepak, Victoria C.   +7 more
core   +1 more source

Inhibition of dipeptidyl peptidase 7 impairs lysophagy in LLOMe‐treated HepG2 cells

FEBS Letters, EarlyView.
DPP7 inhibition delays ubiquitin‐dependent lysophagy by attenuating ubiquitin accumulation and the recruitment of SQSTM1 and LC3 to damaged lysosomes, resulting in delayed clearance of damaged lysosomes and impaired restoration of lysosomal homeostasis. Created using FigureLabs (Certificate ID: FL‐PUB‐20260922‐Z99XNX).
Na Yeon Park, Inho Kwon, Yong Hwan Kim, Ji‐Eun Bae, Joon Bum Kim, Seong Hyun Kim, Sungho Maeng, Doo Sin Jo, Dong‐Hyung Cho   +8 more
wiley   +1 more source

Sensing ligand binding to a clinically relevant lectin by tryptophan fluorescence anisotropy [PDF]

, 2011
Increasing insights into the involvement of endogenous lectins in disease processes fuel the interest to develop potent inhibitors. As a consequence, robust assay procedures are required. Due to their activity as adhesion/growth-regulatory effectors this
Antonia Göhler, Büchner, Claudia, André, Sabine, Sabine André, Kaltner, Herbert, Göhler, Antonia, Gabius, Hans-Joachim, Doose, Sören, Claudia Büchner, Sören Doose, Herbert Kaltner, H.-J. Gabius   +11 more
core   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein

BioTechniques
Avidin-biotin binding, the most robust non-covalent protein-ligand interaction occurring in nature, has wide-ranging applications in biotechnology. A frequent challenge in these applications is accurately determining the number of unoccupied biotin ...
Sonia Mukherjee, Pierre Leblanc, Mark C Poznansky, Ann E Sluder   +3 more
doaj   +1 more source