Results 71 to 80 of about 3,257,031 (299)

Outer membrane protein U (OmpU) mediates adhesion of Vibrio mimicus to host cells via two novel N-terminal motifs.

PLoS ONE, 2015
Vibrio mimicus (V.mimicus) is a causative agent of ascites disease in aquatic animals. Our previous studies have demonstrated that the outer membrane protein U (OmpU) from V.mimicus is an immunoprotective antigen with six immunodominant linear B-cell ...
Xueqin Liu, Huihui Gao, Nin Xiao, Yan Liu, Jinnian Li, Lin Li   +5 more
doaj   +1 more source

Quantitation of lysolipids, fatty acids, and phospholipase A2 activity and correlation with membrane polarity

Journal of Lipid Research, 2012
Acrylodan-labeled rat-intestinal fatty acid binding protein, ADIFAB, binds both of lysophosphatidylcholines (LPC) and FA. Binding displaces Acrylodan and its fluorescence peak shifts from 432 to 505 nm.
Jasmeet Singh, Radha Ranganathan
doaj   +1 more source

A Simple Fluorescence Affinity Assay to Decipher Uranyl‐Binding to Native Proteins

Angewandte Chemie, 2022
AbstractDetermining the affinity of proteins for uranyl is key to understand the toxicity of this cation and to further develop decorporation strategies. However, usual techniques to achieve that goal often require specific equipment and expertise. Here, we propose a simple, efficient, fluorescence‐based method to assess the affinity of proteins and ...
Laporte, Fanny, Chenavier, Yves, Botz, Alexandra, Gateau, Christelle, Lebrun, Colette, Hostachy, Sarah, Vidaud, Claude, Delangle, Pascale   +7 more
openaire   +4 more sources

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Two-photon excitation in fluorescence polarization receptor-ligand binding assay

, 2005
Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds.
Tirri, M E, Huttunen, R J, Toivonen, J, Härkönen, Pirkko, Soini, J T, Hanninen, P E   +5 more
core   +1 more source

ATP changes the fluorescence lifetime of cyan fluorescent protein via an interaction with His148 [PDF]

, 2010
Contains fulltext : 87235.pdf (Publisher’s version ) (Open Access)Recently, we described that ATP induces changes in YFP/CFP fluorescence intensities of Fluorescence Resonance Energy Transfer (FRET) sensors based on CFP-YFP.
Marieke Willemse, Jalink Kees, Fransen, J.A.M., Jalink, K., Sergey P Laptenok, Krogt, G. van der, Krogt, G., Jan Willem Borst, Laptenok Sergey P., Jack A M Fransen, Slijkhuis Rik, Slijkhuis, R., Willemse, M.P., Fransen Jack A. M., Jack A. M. Fransen, Laptenok, S., Willemse, M., Borst, J.W., Wieringa, B., Borst Jan Willem, Gerard van der Krogt, Rik Slijkhuis, Kees Jalink, Sergey P. Laptenok, van der Krogt Gerard, Laptenok, S.P., Be Wieringa, Wieringa Be, Willemse Marieke   +28 more
core   +3 more sources

Fluorescence anisotropy microplate assay for analysis of steroid receptor-DNA interactions

BioTechniques, 2004
To analyze the interactions of steroid/nuclear hormone receptors with their DNA response elements, we used ultra low-volume microplates to develop a simple and rapid fluorescence anisotropy assay. The novel fluorescence anisotropy microplate assay (FAMA)
Stanley Y. Wang, Bonnie S. Ahn, Rebecca Harris, Steven K. Nordeen, David J. Shapiro   +4 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy [PDF]

, 2006
Marme N, Friedrich A, Müller M, et al. Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy. Nucleic Acids Research.
Friedrich, Achim, Sauer, Markus, Nolte, Oliver, Wolfrum, Jürgen, Marme, Nicole, Knemeyer, Jens-Peter, Müller, Matthias, Hoheisel, Jörg D.   +7 more
core   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source