Results 81 to 90 of about 3,434,430 (303)

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

Dengue Envelope Domain III-Peptide Binding Analysis via Tryptophan Fluorescence Quenching Assay

, 2014
In the efforts to find an anti-viral treatment for dengue, a simple tryptophan fluorescence-screening assay aimed at identifying dengue domain III envelope (EIII) protein inhibitors was developed.
Yusof, Rohana, Rahman, Noorsaadah Abdul, Baharuddin, Aida, Tejo, Bimo Ario, Othman, Rozana, Huang, Meilan; id_orcid, Hassan, Asfarina Amir, Xu, Yongtao, Othman, Shatrah   +8 more
core   +2 more sources

Unlocking the potential of cell‐permeable nanobodies for intracellular applications

FEBS Letters, EarlyView.
This review outlines current strategies for delivery of nanobodies into cells and highlights recent examples of functional applications. By addressing and manipulating intracellular targets, these molecules hold great potential for treating cancer, respiratory diseases and neurological disorders, making this a vibrant area of research in the biomedical
Mariona Gené‐Verdés, Luise Franz, Christian P.R. Hackenberger   +2 more
wiley   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Development of a Fluorescence Assay for the Characterization of Brevenal Binding to Rat Brain Synaptosomes

, 2015
: The marine dinoflagellate Karenia brevis produces a family of neurotoxins known as brevetoxins. Brevetoxins elicit their effects by binding to and activating voltage-sensitive sodium channels (VSSCs) in cell membranes. K. brevis also produces brevenal,
Jennifer R. McCall (1336932), Jennifer R. Mccall, Allan J. Goodman (1336938), Alysha M. Thompson (1336941), Allan J. Goodman, Andrea J. Bourdelais, Daniel G. Baden (71736), Andrea J. Bourdelais (71737), Daniel G. Baden, Henry M. Jacocks (1336935), Henry M. Jacocks, Alysha M. Thompson   +11 more
core   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

USP5 Zf-UBD Purification & Fluorescence Polarization Displacement Assay Development

, 2017
USP5 zinc-finger ubiquitin binding domain (Zf-UBD) expression and purification from E. coli, and screening conditions for a fluorescence polarization (FP) displacement assay with a FITC-labelled ubiquitin ...
Arrowsmith, Cheryl (4928326), Edwards, Aled (4929298), Harding, Rachel (4928305), Schapira, Matthieu (4929295), Mann, Mandeep, Harding, Rachel, Mann, Mandeep (4929292), Arrowsmith, Cheryl, Edwards, Aled, Schapira, Matthieu   +9 more
core   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

DNA and BSA binding, DNA cleavage, anticancer activity and In-Silico drug screening study of novel heteroleptic Cu(II) complexes of polypyridyl ligands

European Journal of Medicinal Chemistry Reports
The heteroleptic mononuclear five and six coordinated Copper (II) complexes [CuII(L1)(L2)] [1] and [CuII(L2)(L3)(Cl)][CH3CH2]3NH [2] were prepared and characterised by MASS, IR, EPR and UV-Vis spectroscopic techniques, where L1, L2 and L3 are 1,10 ...
Jansi Rani J, Sovan Roy
doaj   +1 more source

Fluorescence polarization screening for allosteric small molecule ligands of the Cholecystokinin Receptor

, 2011
The success in screening for drug candidates is highly dependent on the power of the strategy implemented. In this work, we report and characterize a novel fluorescent benzodiazepine antagonist of the type 1 cholecystokinin receptor (3-(3-(7-fluoro-1-(2 ...
Kaleeckal G. Harikumar, Miller, LJ, Harikumar, KG, Erin E. Cawston, Laurence J. Miller, Cawston, EE   +5 more
core   +1 more source