Results 91 to 100 of about 3,257,031 (299)

Fluorescence characterization of clinically-important bacteria [PDF]

, 2013
Healthcare-associated infections (HCAI/HAI) represent a substantial threat to patient health during hospitalization and incur billions of dollars additional cost for subsequent treatment.
Jan-Peter Muller, Brody, James P, Roberts Tom A., Lewis R Dartnell, Dartnell, Lewis R., Muller, J-P., Muller, J.P., Tom A Roberts, Muller, JP, Moore, G, Roberts, T.A., Dartnell Lewis R., Ginny Moore, Moore, Ginny, Dartnell, LR, Ward, J.M., Muller Jan-Peter, Moore Ginny, Ward, John M., Moore, G., Ward John M., Roberts, Tom A., Ward, JM, Roberts, TA, Dartnell, L.R., John M Ward   +25 more
core   +2 more sources

DNA and BSA binding, DNA cleavage, anticancer activity and In-Silico drug screening study of novel heteroleptic Cu(II) complexes of polypyridyl ligands

European Journal of Medicinal Chemistry Reports
The heteroleptic mononuclear five and six coordinated Copper (II) complexes [CuII(L1)(L2)] [1] and [CuII(L2)(L3)(Cl)][CH3CH2]3NH [2] were prepared and characterised by MASS, IR, EPR and UV-Vis spectroscopic techniques, where L1, L2 and L3 are 1,10 ...
Jansi Rani J, Sovan Roy
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Simple methods to determine the dissociation constant, Kd

Molecules and Cells
: The determination of the dissociation constant (Kd) is pivotal in biochemistry and pharmacology for understanding binding affinities in chemical reactions, which is crucial for drug development and comprehending biological systems. Here, we introduce a
Donghun Lee, Juwon Kim, Gwangrog Lee
doaj   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

Step-wise assembly, maturation and dynamic behavior of the human CENP-P/O/R/Q/U kinetochore sub-complex [PDF]

, 2012
Kinetochores are multi-protein megadalton assemblies that are required for attachment of microtubules to centromeres and, in turn, the segregation of chromosomes in mitosis. Kinetochore assembly is a cell cycle regulated multi-step process.
Tobias Ulbricht, Deng, Wen, Hellwig Daniela, Hofmeister, Antje, Hellwig, Daniela, Ulbricht Tobias, Döring, Volker, Leonhardt, Heinrich, McAinsh, Andrew D., Wen Deng, Eskat, Anja, James M Bancroft, Emmerth Stephan, Bancroft, James M, Diekmann, Stephan, Bancroft, James M., Volker Döring, Döring Volker, Bancroft James M., Cardoso, M. Cristina, Anja Eskat, Leonhardt Heinrich, Antje Hofmeister, Stephan Emmerth, Christian Hoischen, Rudolphi, Sven, Emmerth, Stephan, Hoischen, Christian, Stephan Diekmann, Rudolphi Sven, Cardoso, M Cristina, Eskat Anja, Heinrich Leonhardt, Hoischen Christian, Sven Rudolphi, Cardoso M. Cristina, Diekmann Stephan, Deng Wen, M Cristina Cardoso, Ulbricht, Tobias, Hofmeister Antje, Meraldi Patrick, McAinsh, Andrew D, Patrick Meraldi, McAinsh Andrew D., Meraldi, Patrick, Daniela Hellwig, Andrew D McAinsh   +47 more
core   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

TAMRA/TAMRA Fluorescence Quenching Systems for the Activity Assay of Alkaline Phosphatase

Sensors, 2017
We introduce two types of fluorescence-quenching assay for alkaline phosphatases (APs) by using a carboxytetramethyl-rhodamine (TAMRA)-labeled phosphate-binding tag molecule (TAMRA-Phos-tag).
Akio Shiba, Emiko Kinoshita-Kikuta, Eiji Kinoshita, Tohru Koike   +3 more
doaj   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Time-resolved fluorescence-based europium-derived probes for peroxidase bioassays, citrate cycle imaging and chirality sensing [PDF]

, 2005
The dissertation describes the development of europium-derived fluorescence probes for biological substances in aqueous solution. A scheme is shown in Fig. 7.1.
Lin, Zhihong
core   +1 more source