Results 61 to 70 of about 3,434,430 (303)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Development of a Competition-Binding Assay to Determine Binding Affinity of Molecules to Neuromelanin via Fluorescence Spectroscopy

Biomolecules, 2019
Neuromelanin, the polymeric form of dopamine which accumulates in aging neuronal tissue, is increasingly recognized as a functional and critical component of a healthy and active adult human brain.
Jackson Fink, Heather Pathak, John Smith, Cindy Achat-Mendes, Robert L. Haining   +4 more
doaj   +1 more source

USP5 Zf-UBD Fluorescence Polarization Displacement Assay #3

, 2018
Development of a fluorescence polarization (FP) assay for USP5 zinc finger ubiquitin binding domain (Zf-UBD) with different lengths of N-terminal FITC-ubiquitin peptides. 
Edwards, Al (5144474), Arrowsmith, Cheryl (4928326), Harding, Rachel (4928305), Schapira, Matthieu (4929295), Mann, Mandeep (4929292), Mann, Mandeep, Harding, Rachel, Arrowsmith, Cheryl, Edwards, Al, Schapira, Matthieu   +9 more
core   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Ankerhold, Richard, Richard Ankerhold, Drummen, Gregor P. C., Gregor P. C. Drummen, Hellen C. Ishikawa-Ankerhold, Ishikawa-Ankerhold, Hellen C.   +5 more
core   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

Screening of G-quadruplex DNA ligands by fluorescence detection of peptide displacement

European Journal of Medicinal Chemistry Reports
G-quadruplexes (G4s) are noncanonical DNA/RNA structures involved in key cellular processes, and their interactions with proteins are emerging as therapeutic targets.
Valentina Arciuolo, Simona Marzano, Rossella Buono, Nicola Grasso, Anna Di Porzio, Antonio Randazzo, Bruno Pagano, Jussara Amato   +7 more
doaj   +1 more source

Translational and rotational motions of albumin sensed by a non-covalent associated porphyrin under physiological and acidic conditions: a fluorescence correlation spectroscopy and time resolved anisotropy study. [PDF]

, 2008
The interaction between a free-base, anionic water-soluble porphyrin, TSPP, and the drug carrier protein, bovine serum albumin (BSA) has been studied by time-resolved fluorescence anisotropy (TRFA) and fluorescence correlation spectroscopy (FCS) at two ...
Costa, S.M., Hoek, A., van, Borst, J.W., Visser, A.J.W.G., Andrade, S.M.   +4 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Outer membrane protein U (OmpU) mediates adhesion of Vibrio mimicus to host cells via two novel N-terminal motifs.

PLoS ONE, 2015
Vibrio mimicus (V.mimicus) is a causative agent of ascites disease in aquatic animals. Our previous studies have demonstrated that the outer membrane protein U (OmpU) from V.mimicus is an immunoprotective antigen with six immunodominant linear B-cell ...
Xueqin Liu, Huihui Gao, Nin Xiao, Yan Liu, Jinnian Li, Lin Li   +5 more
doaj   +1 more source

Quantitation of lysolipids, fatty acids, and phospholipase A2 activity and correlation with membrane polarity

Journal of Lipid Research, 2012
Acrylodan-labeled rat-intestinal fatty acid binding protein, ADIFAB, binds both of lysophosphatidylcholines (LPC) and FA. Binding displaces Acrylodan and its fluorescence peak shifts from 432 to 505 nm.
Jasmeet Singh, Radha Ranganathan
doaj   +1 more source