Results 271 to 280 of about 927,051 (305)

Determination of the drug–DNA binding modes using fluorescence-based assays

Analytical Biochemistry, 2012
Therapeutic drugs and environmental pollutants may exhibit high reactivity toward DNA bases and backbone. Understanding the mechanisms of drug-DNA binding is crucial for predicting their potential genotoxicity. We developed a fluorescence analytical method for the determination of the preferential binding mode for drug-DNA interactions.
Alicia K, Williams, Sofia Cheliout, Dasilva, Ankit, Bhatta, Baibhav, Rawal, Melinda, Liu, Ekaterina A, Korobkova   +5 more
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[33] Fluorescence assay of retinol-binding holoprotein

1980
Publisher Summary This chapter describes fluorescence assay of retinol-binding holoprotein. In assaying retinol in plasma (or serum), it is useful to determine the physiologically active form of the vitamin bound to retinol-binding protein (holoRBP) separately from any other free or esterified retinol that may be present during absorption from the ...
openaire   +1 more source

Fluorescence-based ligand-binding assays for peroxisome proliferator-activated receptors

2002
Publisher Summary The utility of fluorescent-binding assays requires the availability of pure protein for analysis. This determines that the majority of the signal produced is specific to the interaction with the protein under study and also allows for precise control of the amount of receptor present, which is essential for the design and ...
Adamson, Douglas J.A., Palmer, Colin N.A.   +1 more
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Determination of the Extent of Protein Biotinylation by Fluorescence Binding Assay

Bioconjugate Chemistry, 1997
A method was developed to determine the total amount of biotin present in biotinylated protein conjugates. Conjugates of bovine serum albumin, alkaline phosphatase, and horseradish peroxidase were used in this case study. The extent of biotinylation was determined by complete acid hydrolysis or by enzymatic digestion using proteinase K to release ...
S V, Rao, K W, Anderson, L G, Bachas
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Estrogen Receptor Binding Assay Method for Endocrine Disruptors Using Fluorescence Polarization

Analytical Chemistry, 2002
A rapid, simple and nonhazardous assay method for endcrine disruptors was developed using an estrogen receptor (ER) and fluorescence polarization (FP). Among the fluorescent compounds, the 17alpha-fluorescein-labeled estradiol derivative was selected as the most suitable ligand for the ER binding assay, since it showed the highest affinity to ER.
Ken-ichi, Ohno, Takeshi, Fukushima, Tomofumi, Santa, Nobuaki, Waizumi, Hidetoshi, Tokuyama, Masako, Maeda, Kazuhiro, Imai   +6 more
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Peptide Arrays for Highly Sensitive and Specific Antibody-Binding Fluorescence Assays

Bioconjugate Chemistry, 2002
We report a novel generation of peptide arrays fabricated by site-specific ligation of glyoxylyl peptides onto glass slides covered by a semicarbazide sol-gel layer. These arrays allowed the highly sensitive and specific detection of antibodies in very small blood samples from infected individuals using three model peptidic epitopes (HCV Core and NS4 ...
Oleg, Melnyk, Xavier, Duburcq, Christophe, Olivier, Florence, Urbès, Claude, Auriault, Hélène, Gras-Masse   +5 more
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Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes

2007
The authors' laboratory has applied a series of different fluorescence assays for monitoring the binding and transport of fatty acids (FA) in model and biological membranes. The authors recently expanded their fluorescent assays for monitoring the adsorption of FA to membranes to a total of three probes that measure different aspects of FA binding: (1)
Kellen, Brunaldi, Jeffrey R, Simard, Frits, Kamp, Charu, Rewal, Tanong, Asawakarn, Paul, O'Shea, James A, Hamilton   +6 more
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Nucleic Acid-Binding Assay of Argonaute Protein Using Fluorescence Polarization

2017
Nucleic acid binding by the Argonaute protein is an important trigger step in the Argonaute-dependent gene silencing system. We established an in vitro method to detect the nucleic acid binding activity of the Argonaute protein by fluorescence polarization.
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Fluorescence Resonance Energy Transfer-Based Assay for DNA-Binding Protein Tagged by Green Fluorescent Protein

Bioscience, Biotechnology, and Biochemistry, 2006
Specific interaction between green fluorescent protein (GFP)-tagged human alpha- or gamma-enolase(97-242) (alpha or gammaENO(97-242)) and the rhodamine-labeled DNA fragment containing the c-myc P2 promoter was detected by a fluorescence resonance energy transfer (FRET)-based assay, designated as a "real-time FRET assay." The approach of donor (GFP) and
Takashi, Aoki, Tomoko, Imamura, Hiroyuki, Ozaki, Hideki, Ideuchi, Shirou, Tsuchida, Hiroyuki, Watabe   +5 more
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Fluorescent Coupled Enzyme Assays for -Alanine: Application to Penicillin-Binding Protein and Vancomycin Activity Assays

Analytical Biochemistry, 2000
D-Alanine (D-Ala) is a ubiquitous constituent of bacterial cell walls. Assays for D-Ala can be used to investigate several aspects of cell wall biosynthesis and the effects of antibiotics on this process. High-sensitivity fluorescent assays for D-Ala were developed in a microtiter plate format based on d-aminoacid oxidase/horseradish peroxidase (DAO ...
W G, Gutheil, M E, Stefanova, R A, Nicholas   +2 more
openaire   +2 more sources