Results 251 to 260 of about 3,257,031 (299)

Phase separation of DUX family proteins drives totipotent-like state via 3D genome reorganization and retrotransposon activation. [PDF]

Protein Cell
Gao L, Gao Q, Hai N, Wu Z, Li P, Kang H, Song X, Hua J, Zhang S, Ren G, Yang J, Yu L, Wei Y, Ding J, Yang F.   +14 more
europepmc   +1 more source

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers. [PDF]

Anal Chem
Porro G, Cristofori MS, Gagnieux C, Pennati E, Thiriet PE, Constam D, Guiducci C.   +6 more
europepmc   +1 more source

ADORA2A activation restores lysosomal function and photoreceptor outer segment degradation in stressed retinal pigment epithelium. [PDF]

Front Physiol
Tao D, Zhou D, Wu M, Xie P, Zhang Y, Bao J.   +5 more
europepmc   +1 more source

Sensitive detection of unopposed estrogen using estrogen receptor functionalized nanoprobes for cancer diagnosis. [PDF]

Discov Nano
Uti DE, Alum EU, Ogbu CO, Ugwu OP, Egbung GE, Atangwho IJ, Dagnaw M, Jan A.   +7 more
europepmc   +1 more source

Isolation and Characterisation of Phage-Displayed scFv Antibodies Targeting PfHSP70 and PfLDH of <i>Plasmodium falciparum</i>. [PDF]

Biochem Res Int
Gasa NL, Zuma LK, Chiliza TE, Kwezi L, Luthuli SD, Pooe OJ.   +5 more
europepmc   +1 more source

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A fluorescence-based detergent binding assay for protein hydrophobicity

Analytical Biochemistry, 1986
Protein hydrophobicity is often detected by binding of protein to micelles of a mild detergent. A new fluorescence method for detection of this binding is presented. The method is based on a long-range quenching of tryptophan fluorescence by energy transfer to a pyrene-labeled phospholipid probe incorporated into micelles of Brij 96.
Erwin London
exaly   +3 more sources

Phallotoxin and actin binding assay by fluorescence enhancement

Analytical Biochemistry, 1992
The fluorescence of five fluorophores conjugated to phallotoxins was found to be specifically enhanced upon binding to F-actin in a polymerizing buffer. Rhodamine phalloidin had the greatest fluorescence enhancement of ninefold. The fluorescence titration of rhodamine phalloidin by actin was shown to be consistent with stoichiometric binding.
Z J, Huang, R P, Haugland, W M, You, R P, Haugland   +3 more
openaire   +2 more sources

Development of a fluorescence polarization binding assay for asialoglycoprotein receptor

Analytical Biochemistry, 2012
Asialoglycoprotein receptor (ASGP-R) has been actively investigated for targeted delivery of therapeutic agents into hepatocytes because this receptor is selectively and highly expressed in liver and has a high internalization rate. Synthetic cluster glycopeptides (e.g., triGalNAc) bind with high affinity to ASGP-R and, when conjugated to a therapeutic
Anna Y, Kornilova, Bethany, Algayer, Michael, Breslin, Victor, Uebele   +3 more
openaire   +2 more sources

Fluorescent assay for riboflavin binding to cytochrome P450 2B4

Journal of Inorganic Biochemistry, 2004
The interactions between the hemoprotein cytochrome P450 2B4 (CYP 2B4) and riboflavin - a low molecular weight component of the flavoprotein NADPH-dependent cytochrome P450 reductase - were investigated by fluorescence spectroscopy. Riboflavin fluorescence quenching by cytochrome P450 2B4 was used to probe the ligand-enzyme binding (lambda(ex)=385 nm ...
Victoria V, Shumyantseva, Tatiana V, Bulko, Natalia A, Petushkova, Natalia F, Samenkova, Galina P, Kuznetsova, Alexander I, Archakov   +5 more
openaire   +2 more sources

A Fluorescence Polarization Based Src-SH2 Binding Assay

Analytical Biochemistry, 1997
The tyrosine kinase pp60c.src has been implicated as being a potential therapeutic target in several human diseases including cancer and osteoporosis. An important region within this kinase is the SH2 domain (Src homology 2) which binds to phosphorylated tyrosine residues contained within specific peptide sequences.
B A, Lynch, K A, Loiacono, C L, Tiong, S E, Adams, I A, MacNeil   +4 more
openaire   +2 more sources