Results 261 to 270 of about 3,257,031 (299)

Determination of the Extent of Protein Biotinylation by Fluorescence Binding Assay

Bioconjugate Chemistry, 1997
A method was developed to determine the total amount of biotin present in biotinylated protein conjugates. Conjugates of bovine serum albumin, alkaline phosphatase, and horseradish peroxidase were used in this case study. The extent of biotinylation was determined by complete acid hydrolysis or by enzymatic digestion using proteinase K to release ...
S V, Rao, K W, Anderson, L G, Bachas
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A Fluorescence Anisotropy–Based Myt1 Kinase Binding Assay

ASSAY and Drug Development Technologies, 2014
The human Myt1 kinase is a regulator of Cdk1/CycB and, hence, important for the G2/M transition in the cell cycle. It may act as a target for drug development, but suitable assay systems for assessing potential inhibitors are lacking so far.
Alexander, Rohe, Claudia, Henze, Frank, Erdmann, Wolfgang, Sippl, Matthias, Schmidt   +4 more
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Fluorescence Assay for the Binding of Ribonuclease A to the Ribonuclease Inhibitor Protein

Analytical Biochemistry, 2002
Ribonuclease A (RNase A) and the ribonuclease inhibitor protein (RI) form one of the tightest known protein-protein complexes. RNase A variants and homologues, such as G88R RNase A, that retain ribonucleolytic activity in the presence of RI are toxic to cancer cells. Herein, a new and facile assay is described for measuring the equilibrium dissociation
Richele L, Abel, Marcia C, Haigis, Chiwook, Park, Ronald T, Raines   +3 more
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Development of a fluorescence polarization binding assay for folate receptor

Analytical Biochemistry, 2013
Folate receptor (FR) has been actively investigated for targeted delivery of therapeutics into cancer cells because this receptor is selectively and highly expressed in carcinomas. Because FR rapidly cycles between the cell surface and cytoplasm, folic acid conjugated to a therapeutic agent can drive targeted therapeutic delivery to cancer cells.
Anna Y, Kornilova, Bethany, Algayer, Michael, Breslin, George H, Addona, Victor, Uebele   +4 more
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New fluorescent probes for ligand-binding assays of odorant-binding proteins

Biochemical and Biophysical Research Communications, 2014
Fluorescence-linked binding assays allow determination of dissociation constants at equilibrium and have recently become increasingly popular, thanks to their ease of operation. Currently used probes, such as 1-aminoanthracene and N-phenyl-1-naphthylamine, are excited and emit in the ultraviolet region, but alternative ligands operating in the visible ...
Rosa, Mastrogiacomo, Immacolata, Iovinella, Elio, Napolitano   +2 more
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Sterol Binding Assay Using Surface Plasmon Fluorescence Spectroscopy

Analytical Chemistry, 2005
We describe the design of a novel in vitro assay to study the interaction of soluble proteins with small hydrophobic sterol ligands. The sterol molecules are incorporated in an artificial membrane system in order to mimic their arrangement found in a biomembrane.
Wiltschi, B., Schober, M., Kohlwein, S., Oesterhelt, D., Sinner, E.   +4 more
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Probe-dependence of competitive fluorescent ligand binding assays to odorant-binding proteins

Analytical and Bioanalytical Chemistry, 2019
Ligand binding experiments between small chemicals and proteins and the evaluation of dissociation constants of their complexes in competitive binding assays often rely on displacement of reporter probes by the tested ligand. The most widely adopted protocol uses a fluorescent ligand which changes its emission spectrum when bound to a protein.
Jiajun, Tan, Valeriia, Zaremska, Sierin, Lim, Wolfgang, Knoll, Paolo, Pelosi   +4 more
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Fluorescence polarization binding assays for the E3 ligase FEM1C

Feminization-1 homolog C (FEM1C) is a substrate-recognition component of the Cullin 2-RING E3 ubiquitin ligases (CRL2), selectively binding the C-degron containing arginine-terminated motifs to mediate proteasomal degradation of target proteins. Owing to its wide expression, FEM1C holds promise for targeted protein degradation.
Emma K, Seipp, Rong, Huang
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Fluorescent Ligands for the Hemagglutinin of Influenza A:  Synthesis and Ligand Binding Assays

Bioconjugate Chemistry, 2006
Two fluorescent conjugates of sialic acid have been prepared, with a convenient synthetic route that involves preparation of an unsaturated benzyl ester by cross-metathesis, followed by combined hydrogenation/ hydrogenolysis to provide a sialoside bearing a delta-carboxybutyl group, suitable for coupling with the chosen fluorophores.
V Ranjit N, Munasinghe, John E T, Corrie, Geoff, Kelly, Stephen R, Martin   +3 more
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A High-Throughput STAT Binding Assay Using Fluorescence Polarization

Analytical Biochemistry, 1997
STAT (signal transducers and activators of transcription) is a class of transcription factors that are activated upon cytokine or growth factor binding to cell surface receptors. Activated STAT proteins dimerize, translocate into the nucleus, and activate transcription, leading to various immune responses.
P, Wu, M, Brasseur, U, Schindler
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