Results 271 to 280 of about 3,257,031 (299)

Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors

2017
Binding assay is a common technique used to characterize ability of a ligand to interact with a specific biological target. A number of parameters, such as binding affinity, receptor density, and association/dissociation rate constants, can be measured by means of this technique.
Bragina ME, Stergiopulos N, Fraga-Silva RA   +2 more
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A Fluorescence‐Lifetime‐Based Binding Assay for Class IIa Histone Deacetylases

Chemistry – A European Journal, 2017
AbstractClass IIa histone deacetylases (HDACs) show extremely low enzymatic activity and no commonly accepted endogenous substrate is known today. Increasing evidence suggests that these enzymes exert their effect rather through molecular recognition of acetylated proteins and recruiting other proteins like HDAC3 to the desired target location ...
Christian Meyners, Monique Mertens, Pablo Wessig, Franz‐Josef Meyer‐Almes   +3 more
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Determination of the drug–DNA binding modes using fluorescence-based assays

Analytical Biochemistry, 2012
Therapeutic drugs and environmental pollutants may exhibit high reactivity toward DNA bases and backbone. Understanding the mechanisms of drug-DNA binding is crucial for predicting their potential genotoxicity. We developed a fluorescence analytical method for the determination of the preferential binding mode for drug-DNA interactions.
Alicia K, Williams, Sofia Cheliout, Dasilva, Ankit, Bhatta, Baibhav, Rawal, Melinda, Liu, Ekaterina A, Korobkova   +5 more
openaire   +2 more sources

A Competition Assay for DNA Binding Using the Fluorescent Probe ANS

2003
Fluorescence spectroscopy is a technique frequently employed to study protein-nucleic acid interactions. Often, the intrinsic fluorescence emission spectrum of tryptophan residues in a nucleic-acid-binding protein is strongly perturbed upon interaction with a target DNA or RNA.
Taylor, I., Kneale, Geoff
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[33] Fluorescence assay of retinol-binding holoprotein

1980
Publisher Summary This chapter describes fluorescence assay of retinol-binding holoprotein. In assaying retinol in plasma (or serum), it is useful to determine the physiologically active form of the vitamin bound to retinol-binding protein (holoRBP) separately from any other free or esterified retinol that may be present during absorption from the ...
openaire   +1 more source

Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes

2007
The authors' laboratory has applied a series of different fluorescence assays for monitoring the binding and transport of fatty acids (FA) in model and biological membranes. The authors recently expanded their fluorescent assays for monitoring the adsorption of FA to membranes to a total of three probes that measure different aspects of FA binding: (1)
Kellen, Brunaldi, Jeffrey R, Simard, Frits, Kamp, Charu, Rewal, Tanong, Asawakarn, Paul, O'Shea, James A, Hamilton   +6 more
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Recombinant Green Fluorescent Protein as a Label in Binding Assays

Analytical Biochemistry, 1998
E C, Hernández, S, Daunert
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Creation of Fluorescent RXR Antagonists Based on CBTF-EE and Application to a Fluorescence Polarization Binding Assay

ACS Medicinal Chemistry Letters, 2021
Yuta Takamura, Hiroki Kakuta, Shogo Nakano   +2 more
exaly  

Nucleic Acid-Binding Assay of Argonaute Protein Using Fluorescence Polarization

2017
Nucleic acid binding by the Argonaute protein is an important trigger step in the Argonaute-dependent gene silencing system. We established an in vitro method to detect the nucleic acid binding activity of the Argonaute protein by fluorescence polarization.
openaire   +2 more sources

A fluorescent assay for serum albumin binding capacity

Toxicology Letters, 1995
Yu.I. Miller, Yu.A. Gryzunov, G.E. Dobretsov   +2 more
openaire   +2 more sources