Background Malignant lymphomas are classified based on morphology, immunophenotype, genetics and clinical features. The pathological diagnosis is generally considered difficult and prone to mistakes.
Anke van Rijk+10 more
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FISH molecular testing in cytological preparations from solid tumors [PDF]
Many of the exciting new developments in solid tumor molecular cytogenetics impact classical and molecular pathology. Fluorescence in situ hybridization to identify specific DNA target sequences in nuclei of non-dividing cells in solid neoplasms has ...
CARIA, PAOLA, VANNI, ROBERTA
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Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson+15 more
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This paper aimed to understand and compare the two popular cytogenetic techniques of fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in detecting breast cancer chromosomal abnormality.
Harem Othman Smail
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Historical perspective of in situ hybridization for the analysis of genomic constitution of plants [PDF]
In situ hybridization involves hybridization of DNA or RNA probes to the cytological preparations. The technique originally used auto-radiographic labeling to map both repetitive and low copy DNA sequences.
Abbasi, FM+7 more
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Infantile nodular fasciitis of the hand: A case report and literature review
Pediatric nodular fasciitis is uncommon and has a preference for the head and neck region. Occurrence in other anatomic locations is uncommon. We describe here a case of nodular fasciitis that arose in the hand of a newborn infant who presented with a ...
I Weng Lao+3 more
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Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization
Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence.
Yousuke Tsuneoka+8 more
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Determination of HER2 amplification in primary breast cancer using dual-colour chromogenic in situ hybridization is comparable to fluorescence in situ hybridization: a European multicentre study involving 168 specimens [PDF]
García-Caballero T, Grabau D, Green A R, Gregory J, Schad A, Kohlwes E, Ellis I O, Watts S & Mollerup J (2010) Histopathology56, 472–480 Determination of HER2 amplification in primary breast cancer using dual-colour chromogenic in situ hybridization is ...
Arnould+26 more
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Localization of the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to 8q22 by in situ hybridization [PDF]
We have localized the human gene encoding the 13.3-kDa subunit of mitochondrial complex III (UQCRB) to chromosome 8 using both radioactive in situ hybridization and fluorescence in situ hybridization.
Heng, HHQ+4 more
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A simplified and rapid in situ hybridization protocol for planarians
Whole-mount in situ hybridization is a critical technique for analyzing gene expression in planarians. While robust in situ protocols have been developed, these protocols are laborious, making them challenging to incorporate in an academic setting ...
Andrew J Gaetano, Ryan S King
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