Results 11 to 20 of about 129,094 (159)

Fluorescence In Situ Hybridization [PDF]

open access: yesNature Methods, 2005
Dr. Seuss’s eloquent “One FISH, two FISH, red FISH, blue FISH” (1) could have been describing one of the most significant advancements in clinical cytogenetics, fluorescence in situ hybridization (FISH). The process, as described by Pinkel et al. in 1988 (2), involved fluorescent detection of probe DNA hybridized to chromosomal target sequences.
Daynna J. Wolff, Stuart Schwartz
  +4 more sources

Infantile nodular fasciitis of the hand: A case report and literature review

open access: yesHuman Pathology: Case Reports, 2016
Pediatric nodular fasciitis is uncommon and has a preference for the head and neck region. Occurrence in other anatomic locations is uncommon. We describe here a case of nodular fasciitis that arose in the hand of a newborn infant who presented with a ...
I Weng Lao   +3 more
doaj   +1 more source

The Roles of the Fluorescent In Situ Hybridization (FISH) and Comparative Genomic Hybridization (CGH) Techniques in the Detection of the Breast Cancer

open access: yesBiology, Medicine & Natural Product Chemistry, 2022
This paper aimed to understand and compare the two popular cytogenetic techniques of fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in detecting breast cancer chromosomal abnormality.
Harem Othman Smail
doaj   +1 more source

HER 2 status in invasive breast cancer: Immunohistochemistry, fluorescence in-situ hybridization and chromogenic in-situ hybridization

open access: yesIndian Journal of Pathology and Microbiology, 2012
Introduction : HER2/neu gene status in breast cancers can be evaluated by targeting protein and gene - immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH).
Hemlata S Shirsat   +5 more
doaj   +1 more source

Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization

open access: yesFrontiers in Molecular Neuroscience, 2022
Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence.
Yousuke Tsuneoka   +8 more
doaj   +1 more source

Fluorescence in situ hybridization improves the detection of 5q31 deletion in myelodysplastic syndromes without cytogenetic evidence of 5q-

open access: yesHaematologica, 2008
Background More than 50% of patients with myelodysplastic syndromes present cytogenetic aberrations at diagnosis. Partial or complete deletion of the long arm of chromosome 5 is the most frequent abnormality.
Mar Mallo   +41 more
doaj   +1 more source

A simplified and rapid in situ hybridization protocol for planarians

open access: yesBioTechniques, 2023
Whole-mount in situ hybridization is a critical technique for analyzing gene expression in planarians. While robust in situ protocols have been developed, these protocols are laborious, making them challenging to incorporate in an academic setting ...
Andrew J Gaetano, Ryan S King
doaj   +1 more source

Fluorescent in Situ Hybridization [PDF]

open access: yes, 2008
In situ hybridization (ISH) technique was introduced by Gall and Pardue1 in 1969. At that time the technique was limited by the use of radioactively labelled probes that were subsequently visualized by autoradiography. The development of interphase cytogenetics in the 1980s and fluorescent labels in 19862 has seen the technology applied in a number of ...
Fiona Campbell, John M. S. Bartlett
openaire   +2 more sources

Fluorescence in situ Hybridization (FISH) in the Microbiological Diagnostic of Deep Sternal Wound Infection (DSWI)

open access: yesInfection and Drug Resistance, 2021
Nick Spindler,1 Annette Moter,2,3 Alexandra Wiessner,2,3 Tanja Gradistanac,4 Michael Borger,5 Arne C Rodloff,6 Stefan Langer,1 Judith Kikhney2,3 1Department of Orthopedic Surgery, Traumatology and Plastic Surgery, University Hospital Leipzig, Leipzig ...
Spindler N   +7 more
doaj  

Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women

open access: yesBrazilian Oral Research, 2012
The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women.
Fernanda Campos Machado   +4 more
doaj   +1 more source

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