Results 11 to 20 of about 135,291 (301)

In situ visualization of bacterial populations in coral tissues: pitfalls and solutions [PDF]

PeerJ, 2016
In situ visualization of microbial communities within their natural habitats provides a powerful approach to explore complex interactions between microorganisms and their macroscopic hosts.
Naohisa Wada, Frederic J. Pollock, Bette L. Willis, Tracy Ainsworth, Nobuhiro Mano, David G. Bourne   +5 more
doaj   +2 more sources

Fluorescence In Situ Hybridization Method for Co-Localization of mRNA and GFP

BioTechniques, 2001
Co-localization studies using green fluorescent protein (GFP) and fluorescence immunohistochemistry have become commonplace. However, co-localization studies using GFP and mRNA in situ hybridization are rare, in large part because typical in situ ...
Anthony A. Oliva, John W. Swann
doaj   +1 more source

Double-staining chromogenic in situ hybridization as a useful alternative to split-signal fluorescence in situ hybridization in lymphoma diagnostics

Haematologica, 2010
Background Malignant lymphomas are classified based on morphology, immunophenotype, genetics and clinical features. The pathological diagnosis is generally considered difficult and prone to mistakes.
Anke van Rijk, Tim Svenstroup-Poulsen, Margaret Jones, José Cabeçadas, Juan Cruz Cigudosa, Lorenzo Leoncini, Anja Mottok, Christiane Copie Bergman, Evi Pouliou, Stephen Hamilton Dutoit, Han J. van Krieken   +10 more
doaj   +1 more source

Fluorescence In Situ Hybridization [PDF]

Nature Methods, 2005
Dr. Seuss’s eloquent “One FISH, two FISH, red FISH, blue FISH” (1) could have been describing one of the most significant advancements in clinical cytogenetics, fluorescence in situ hybridization (FISH). The process, as described by Pinkel et al. in 1988 (2), involved fluorescent detection of probe DNA hybridized to chromosomal target sequences.
Daynna J. Wolff, Stuart Schwartz
  +4 more sources

The Roles of the Fluorescent In Situ Hybridization (FISH) and Comparative Genomic Hybridization (CGH) Techniques in the Detection of the Breast Cancer

Biology, Medicine & Natural Product Chemistry, 2022
This paper aimed to understand and compare the two popular cytogenetic techniques of fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in detecting breast cancer chromosomal abnormality.
Harem Othman Smail
doaj   +1 more source

Infantile nodular fasciitis of the hand: A case report and literature review

Human Pathology: Case Reports, 2016
Pediatric nodular fasciitis is uncommon and has a preference for the head and neck region. Occurrence in other anatomic locations is uncommon. We describe here a case of nodular fasciitis that arose in the hand of a newborn infant who presented with a ...
I Weng Lao, Xiaohang Liu, Lin Yu, Jian Wang   +3 more
doaj   +1 more source

HER 2 status in invasive breast cancer: Immunohistochemistry, fluorescence in-situ hybridization and chromogenic in-situ hybridization

Indian Journal of Pathology and Microbiology, 2012
Introduction : HER2/neu gene status in breast cancers can be evaluated by targeting protein and gene - immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH).
Hemlata S Shirsat, Sridhar Epari, Tanuja Shet, Rajani Bagal, Rohini Hawaldar, Sangeeta B Desai   +5 more
doaj   +1 more source

Fluorescence in situ hybridization improves the detection of 5q31 deletion in myelodysplastic syndromes without cytogenetic evidence of 5q-

Haematologica, 2008
Background More than 50% of patients with myelodysplastic syndromes present cytogenetic aberrations at diagnosis. Partial or complete deletion of the long arm of chromosome 5 is the most frequent abnormality.
Mar Mallo, Leonor Arenillas, Blanca Espinet, Marta Salido, Jesús Mª Hernández, Eva Lumbreras, Mónica del Rey, Eva Arranz, Soraya Ramiro, Patricia Font, Olga González, Mónica Renedo, José Cervera, Esperanza Such, Guillermo F. Sanz, Elisa Luño, Carmen Sanzo, Miriam González, María José Calasanz, José Mayans, Carlos García-Ballesteros, Victoria Amigo, Rosa Collado, Isabel Oliver, Félix Carbonell, Encarna Bureo, Andrés Insunza, Lucrecia Yañez, María José Muruzabal, Elena Gómez-Beltrán, Rafael Andreu, Pilar León, Valle Gómez, Ángeles Sanz, Natalia Casasola, Esperanza Moreno, Adrián Alegre, María Luisa Martín, Carmen Pedro, Sergi Serrano, Lourdes Florensa, Francesc Solé   +41 more
doaj   +1 more source

A simplified and rapid in situ hybridization protocol for planarians

BioTechniques, 2023
Whole-mount in situ hybridization is a critical technique for analyzing gene expression in planarians. While robust in situ protocols have been developed, these protocols are laborious, making them challenging to incorporate in an academic setting ...
Andrew J Gaetano, Ryan S King
doaj   +1 more source

Fluorescent in Situ Hybridization [PDF]

, 2008
In situ hybridization (ISH) technique was introduced by Gall and Pardue1 in 1969. At that time the technique was limited by the use of radioactively labelled probes that were subsequently visualized by autoradiography. The development of interphase cytogenetics in the 1980s and fluorescent labels in 19862 has seen the technology applied in a number of ...
Fiona Campbell, John M. S. Bartlett
openaire   +2 more sources