Results 41 to 50 of about 129,094 (159)
Protocol optimization improves the performance of multiplexed RNA imaging
Scientific ReportsSpatial transcriptomics has emerged as a powerful tool to define the cellular structure of diverse tissues. One such method is multiplexed error robust fluorescence in situ hybridization (MERFISH).
Josh J. Luce +10 more
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Problems In Determining Her2 Status In Breast Carcinoma
European Journal of Breast Health, 2015 Objective:Human epidermal growth factor receptor 2 (HER2) oncoprotein is overexpressed in 15-25% of breast carcinomas and associated with poor outcome.
Emel Ebru Pala +5 more
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Taiwanese Journal of Obstetrics & Gynecology, 2006 Objective: The aim of this study was to determine the genetic composition of tripronuclear (3PN) zygotes. Fluorescence in situ hybridization (FISH) was used to quantify the sperm tails in human 3PN zygotes. Materials and Methods: Ovarian hyperstimulation
San-Li Yu +6 more
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Testing the Potential Clastogenic/Cytotoxic Effects of Pesticide CALYPSO 480 SC
Folia Veterinaria, 2017 The detection of chromosomal damage serves as a tool for the verification of the genotoxic effects of chemical substances in vitro. We used conventional cytogenetic analysis in order to test for the potential genotoxic action of the insecticide ...
Ficová I., Galdíková M.
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Taiwanese Journal of Obstetrics & Gynecology, 2015 Objective: To evaluate how interphase fluorescence in situ hybridization (FISH) played a role in genetic counseling when encountering prenatally detected fetal mosaicism cases. Materials and methods: We retrospectively reviewed 17 cases of amniotic fluid
Sheng-Yuan Su +5 more
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Acta Medica Bulgarica, 2017 Molecular biology of esophageal adenocarcinoma (EAC) is not fully elucidated. The aim of this study was to assess the expression of cycle regulator and tumor suppressor p16 in esophageal adenocarcinoma. The expression of p16 at protein and gene level was
Kotzev A., Kamenova M.
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A prospective comparison of UroVysion FISH and urine cytology in bladder cancer detection
BMC Cancer, 2017 Background UroVysion fluorescence in situ hybridization (uFISH) was reported to have superior sensitivity to urine cytology. However uFISH studies are limited by varying definitions of what is considered a positive result, absence of histopathology and ...
Hugh J. Lavery +4 more
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A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection
Science and Technology of Advanced Materials, 2016 We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target,
Hiromu Kashida +2 more
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Fluorescent in situ hybridization has limitations in screening NRG1 gene rearrangements
Diagnostic PathologyBackground NRG1 fusion is a promising therapeutic target for various tumors but its prevalence is extremely low, and there are no standardized testing algorithms for genetic assessment. Mothods In this study, we analyzed 3008 tumors using Fluorescence in
Xiaomei Zhang +9 more
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Вавиловский журнал генетики и селекции, 2014 Presently, suppression of repetitive DNAsequences (chromosomal in situ suppression hybridization, СISS-hybridization) is used to improve the results of fluorescence in situ hybridization (FISH). However, in some cases the suppression cannot be performed,
A. G. Bogomolov +4 more
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