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Fluorescence lifetime-resolved imaging
Photosynthesis Research, 2009This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the ...
Yi-Chun, Chen, Robert M, Clegg
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Fluorescence Lifetime Imaging Microscopy
Current Protocols in Cell Biology, 2004AbstractFluorescent lifetime imaging microscopy is a powerful tool to enhance the contrast in images of biological samples and to investigate the local environment of a fluorochrome. FLIM allows the detection of protein‐protein interactions and their biochemical state by the quantitative detection of Förster resonance energy transfer (FRET) between ...
Alessandro, Esposito, Fred S, Wouters
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Fluorescence Lifetime Imaging Microscopy
2007Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially resolved images of fluorophore lifetime (the property describing how rapidly fluorescence decays), providing another dimension of information for visualizing fluorophores and an additional source of contrast.
Ching-Wei, Chang +2 more
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Video rate fluorescence lifetime imaging and fluorescence lifetime endoscopy
SPIE Proceedings, 2005We report real-time (video-rate) fluorescence lifetime imaging and its application to tissue autofluorescence and endoscopy, demonstrating FLIM of unstained ex vivo tissue at update rates of 5.5 Hz through a flexible endoscope.
James McGinty +8 more
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Rapid hyperspectral fluorescence lifetime imaging
Microscopy Research and Technique, 2007AbstractWe report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high‐speed FLIM technology in a line‐scanning microscope. We demonstrate the acquisition of whole‐field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to ...
Pieter, De Beule +13 more
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2014
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation ...
Suhling, Klaus +11 more
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Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation ...
Suhling, Klaus +11 more
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Fluorescence lifetime imaging of coral fluorescent proteins
Microscopy Research and Technique, 2007AbstractCorals, like many other coelenterates, contain fluorescent pigments that show considerable homology with the well known green fluorescent protein of the jellyfish Aequoria. In corals, unlike jellyfish, multiple proteins are present and the range of excitations and emissions suggest the possibility of energy transfer.
Guy, Cox, Mikhail, Matz, Anya, Salih
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Fourier Multiplexed Fluorescence Lifetime Imaging
2021Fluorescence lifetime imaging microscopy (FLIM) is a widely used functional imaging method in bioscience. Fourier multiplexed FLIM (FmFLIM), a frequency-domain lifetime measurement method, explores the principle of Fourier (frequency) multiplexing to achieve parallel lifetime detection on multiple fluorescence labels.
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Fluorescence Lifetime Imaging Microscopy (FLIM)
2005Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems have been implemented both in the frequency domain, using sinusoidally intensity-modulated excitation light and modulated detectors, and in the time domain, using pulsed excitation ...
Erik B, van Munster +1 more
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Confocal Fluorescence Lifetime Imaging
1996The optical microscope, arguably the most mature of all laboratory instruments, is currently enjoying a period of intense development that is opening up many new application areas. These developments have been made possible by the use of new optical arrangements, sophisticated light sources and detectors and by the application of novel fluorescence ...
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