Results 261 to 270 of about 114,370 (293)

Tomographic Fluorescence Lifetime Imaging

Biomedical Optics and 3-D Imaging, 2012
We present theory and methods for in vivo diffuse time domain fluorescence lifetime tomography. As examples, we will discuss in vivo lifetime imaging of both targeted and activatable probes in mouse models of cardiac disease and cancer.
Scott B. Raymond, Craig J. Goergen, Alexei Bogdanov, David E. Sosnovik, Anand T. N. Kumar   +4 more
openaire   +1 more source

Fluorescence lifetime imaging microscopy

Biomedical Optical Spectroscopy and Diagnostics, 2000
We demonstrate the application of a time-domain 2D fluorescence lifetime imaging (FLIM) system to microscopy of biological tissue. We report the extension of this work to 3D FLIM microscopy.
M. J. Cole, J. Siegel, R. Jones, S. E. D. Webb, Y. Gu, P. M. W. French, M. J. Lever, M. A. A. Neil, R. Juškaitis, T. Wilson   +9 more
openaire   +1 more source

Fluorescence Lifetime Imaging Microscopy: Two‐Dimensional Distribution Measurement of Fluorescence Lifetime

2006
A newly developed fluorescence lifetime imaging microscopy (FLIM) system has combined the high-temporal resolution of a streak camera with the high-spatial resolution of a microscope to obtain a two-dimensional distribution of fluorescence lifetimes within living cells. The temporal resolution is as short as 20 ps.
Masanobu, Fujiwara, William, Cieslik
openaire   +2 more sources

Fluorescence Lifetime Imaging Microscopy

1999
The marriage of fluorescence microscopy with digital imaging has resulted in an expansion in our understanding of cellular function. Currently, most fluorescence microscopic imaging is performed as a measurement of emission or excitation intensity. These types of measurements have several limitations: a) intensity-based fluorescence imaging can be ...
B. Herman, X. F. Wang, P. Wodnicki, A. Perisamy, N. Mahajan, G. Berry, G. Gordon   +6 more
openaire   +1 more source

Multispectral fluorescence lifetime imaging by TCSPC

Microscopy Research and Technique, 2007
AbstractWe present a fluorescence lifetime imaging technique with simultaneous spectral and temporal resolution. The technique is fully compatible with the commonly used multiphoton microscopes and nondescanned (direct) detection. An image of the back‐aperture of the microscope lens is projected on the input of a fiber bundle.
Wolfgang, Becker, Axel, Bergmann, Christoph, Biskup   +2 more
openaire   +2 more sources

Multiple frequency fluorescence lifetime imaging microscopy

Journal of Microscopy, 2000
The experimental configuration and the computational algorithms for performing multiple frequency fluorescence lifetime imaging microscopy (mfFLIM) are described. The mfFLIM experimental set‐up enables the simultaneous homodyne detection of fluorescence emission modulated at a set of harmonic frequencies.
Squire, A., Verveer, P., Bastiaens, P.
openaire   +3 more sources

Spectrally Resolved Fluorescence Lifetime Imaging Microscopy

Applied Spectroscopy, 2002
We report a system for collecting spectrally resolved fluorescent lifetime images. Frequency domain fluorescence lifetime detection was combined with two-dimensional spectral imaging in a programmable array microscope. The spectroscopic fluorescence lifetime imaging microscopy (sFLIM) system has a resolution of ∼50 (λ/Δλ) in the current arrangement ...
Hanley, Q., Arndt-Jovin, D., Jovin, T.
openaire   +2 more sources

Fluorescence Lifetime Imaging of the Skin

2015
Skin research and clinical dermatological diagnoses have traditionally relied on morphological assessment obtained by various imaging techniques. With the advent of various new technologies, including polarised, confocal, multiphoton, infrared, photoacoustic, sonographic and magnetic resonance techniques, there has been a major step forward in non ...
Washington Y. Sanchez, Michael Pastore, Isha N. Haridass, Karsten König, Wolfgang Becker, Michael S. Roberts   +5 more
openaire   +2 more sources

Fluorescence Lifetime Imaging Microscopy

2019
FLIO is based on Fluorescence Lifetime Imaging Microscopy (FLIM), which is used in basic science to investigate metabolic conditions in cell cultures and tissues. After excitation of fluorophores with a laser light, the fluorophore returns to its ground state by releasing light with a longer wavelength.
openaire   +1 more source

Lifetime-resolved fluorescence imaging

1994
Fluorescence lifetime imaging is a relatively new technique for acquiring directly the nanosecond temporal characteristics of the fluorescence emission of a spatially extended object, and for capturing the dynamic features at every pixel of an image simultaneously.
Clegg, M.R., Gadella, T.W.J., Jovin, T.M.   +2 more
openaire   +2 more sources