Results 111 to 120 of about 30,206 (151)

Fluorescence Lifetime Imaging Microscopy

Current Protocols in Cell Biology, 2004
AbstractFluorescent lifetime imaging microscopy is a powerful tool to enhance the contrast in images of biological samples and to investigate the local environment of a fluorochrome. FLIM allows the detection of protein‐protein interactions and their biochemical state by the quantitative detection of Förster resonance energy transfer (FRET) between ...
Alessandro, Esposito, Fred S, Wouters
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Spectrally Resolved Fluorescence Lifetime Imaging Microscopy

Applied Spectroscopy, 2002
We report a system for collecting spectrally resolved fluorescent lifetime images. Frequency domain fluorescence lifetime detection was combined with two-dimensional spectral imaging in a programmable array microscope. The spectroscopic fluorescence lifetime imaging microscopy (sFLIM) system has a resolution of ∼50 (λ/Δλ) in the current arrangement and
Thomas M Jovin, Quentin S. Hanley, Donna J Arndt-Jovin   +2 more
exaly   +3 more sources

Fluorescence Lifetime Imaging Microscopy (FLIM)

2005
Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems have been implemented both in the frequency domain, using sinusoidally intensity-modulated excitation light and modulated detectors, and in the time domain, using pulsed excitation ...
Erik B, van Munster, Theodorus W J, Gadella   +1 more
openaire   +4 more sources

Fluorescence Lifetime Imaging Microscopy

2007
Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially resolved images of fluorophore lifetime (the property describing how rapidly fluorescence decays), providing another dimension of information for visualizing fluorophores and an additional source of contrast.
Ching-Wei, Chang, Dhruv, Sud, Mary-Ann, Mycek   +2 more
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Fluorescence lifetime imaging microscopy

Biomedical Optical Spectroscopy and Diagnostics, 2000
We demonstrate the application of a time-domain 2D fluorescence lifetime imaging (FLIM) system to microscopy of biological tissue. We report the extension of this work to 3D FLIM microscopy.
M. J. Cole, J. Siegel, R. Jones, S. E. D. Webb, Y. Gu, P. M. W. French, M. J. Lever, M. A. A. Neil, R. Juškaitis, T. Wilson   +9 more
openaire   +1 more source

Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging Microscopy

Nephron Experimental Nephrology, 2006
With few and commercially available add-ons, both confocal and full-field fluorescence microscopes can be adapted to provide more information on the biological sample of interest. In this review we discuss the possibilities offered by two additional functionalities to fluorescence microscopes, fluorescence correlation spectroscopy (FCS) and ...
Sophia Y, Breusegem, Moshe, Levi, Nicholas P, Barry   +2 more
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Fluorescence lifetime imaging: an emerging technique in fluorescence microscopy

Chromosome Research, 1996
Fluorescence microscopy is an important tool for biological research, in part because of the extremely high detection sensitivity that can be achieved, but also because fluorescent molecules can be used as probes on account of their environmental responsiveness, for example to measure intracellular pH or metal ion concentration.
C G, Morgan, A C, Mitchell
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Multiple frequency fluorescence lifetime imaging microscopy

Journal of Microscopy, 2000
The experimental configuration and the computational algorithms for performing multiple frequency fluorescence lifetime imaging microscopy (mfFLIM) are described. The mfFLIM experimental set‐up enables the simultaneous homodyne detection of fluorescence emission modulated at a set of harmonic frequencies.
Squire, A., Verveer, P., Bastiaens, P.
openaire   +3 more sources

Fluorescence Lifetime Imaging in Scanning Microscopy

2006
Third ...
Gerritsen, H.C., Draaijer, A., Heuvel, D.J. van den, Agronskaia, A.V.   +3 more
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Fluorescence Lifetime Imaging Microscopy

1999
The marriage of fluorescence microscopy with digital imaging has resulted in an expansion in our understanding of cellular function. Currently, most fluorescence microscopic imaging is performed as a measurement of emission or excitation intensity. These types of measurements have several limitations: a) intensity-based fluorescence imaging can be ...
B. Herman, X. F. Wang, P. Wodnicki, A. Perisamy, N. Mahajan, G. Berry, G. Gordon   +6 more
openaire   +1 more source