Results 11 to 20 of about 1,049,609 (265)
Fluorescence antibunching microscopy [PDF]
, 2011 Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of fluorescent markers, routinely used as contrast labels for bio-imaging.
D. Oron, M. Kendall, O. Schwartz
arxiv +3 more sources
Probing subtle fluorescence dynamics in cellular proteins by streak camera based Fluorescence Lifetime Imaging Microscopy [PDF]
, 2003 We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown to have high accuracy and reproducibility.
Biener, Eva +4 more
arxiv +3 more sources
Three-dimensional virtual refocusing of fluorescence microscopy images using deep learning [PDF]
Nature Methods (2019), 2019 Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D fluorescence image onto user-defined 3D surfaces within the sample volume.
Ben-David, Eyal +7 more
arxiv +3 more sources
Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells [PDF]
Opt. Lett. 43, 2587-2590 (2018), 2019 We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally positioned at the exit of an optical microscope.
Barnea, Itay +3 more
arxiv +3 more sources
Exploiting speckle correlations to improve the resolution of wide-field fluorescence microscopy [PDF]
Optica, Vol. 2, Issue 5, pp. 424-429 (2015), 2014 Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field of view. Due to the diffraction limit, conventional optical microscopes are limited to resolving structures larger ...
Bertolotti, Jacopo +5 more
arxiv +7 more sources
Advanced Methods in Fluorescence Microscopy [PDF]
Analytical Cellular Pathology, 2013 It requires a good deal of will power to resist hyperbole in considering the advances that have been achieved in fluorescence microscopy in the last 25 years.
Luke Fritzky, David Lagunoff
doaj +5 more sources
Calibrating Fluorescence Microscopy With 3D-Speckler (3D Fluorescence Speckle Analyzer) [PDF]
Bio-ProtocolFluorescence microscopy has been widely accessible and indispensable in cell biology research. This technique enables researchers to label targets, ranging from individual entities to multiple groups, with fluorescent markers.
Chieh-Chang Lin, Aussie Suzuki
doaj +2 more sources
Fluorescence microscopy shadow imaging for neuroscience [PDF]
Frontiers in Cellular NeuroscienceFluorescence microscopy remains one of the single most widely applied experimental approaches in neuroscience and beyond and is continuously evolving to make it easier and more versatile.
V. V. G. Krishna Inavalli +8 more
doaj +2 more sources
Cold Spring Harbor Protocols, 2014 Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution.
Sanderson, Michael J +3 more
openaire +6 more sources
Linear Combination Properties of the Phasor Space in Fluorescence Imaging
Sensors, 2022 The phasor approach to fluorescence lifetime imaging, and more recently hyperspectral fluorescence imaging, has increased the use of these techniques, and improved the ease and intuitiveness of the data analysis.
Belén Torrado +2 more
doaj +1 more source