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Recurrent neural network-based volumetric fluorescence microscopy
Volumetric imaging of samples using fluorescence microscopy plays an important role in various fields including physical, medical and life sciences. Here we report a deep learning-based volumetric image inference framework that uses 2D images that are ...
Luzhe Huang +4 more
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Intracellular processes depend on a strict spatial and temporal organization of proteins and organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is crucial in understanding cellular physiology.
Sergey V. Loginov +9 more
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Plasmonics Enhanced Smartphone Fluorescence Microscopy
Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses.
Qingshan Wei +9 more
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Fluorescence Microscopy of the HIV-1 Envelope
Human immunodeficiency virus (HIV) infection constitutes a major health and social issue worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane.
Pablo Carravilla +2 more
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Scanless multitarget-matching multiphoton excitation fluorescence microscopy [PDF]
Using the combination of a reflective blazed grating and a reflective phase-only diffractive spatial light modulator (SLM), scanless multitarget-matching multiphoton excitation fluorescence microscopy (SMTM-MPM) was achieved.
Junpeng Qiu +4 more
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Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest.
Sébastien, Peter +2 more
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Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations
Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell ...
Shane M. Hickey +12 more
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Epi-Fluorescence Microscopy [PDF]
Epi-fluorescence microscopy is available in most life sciences research laboratories, and when optimized can be a central laboratory tool. In this chapter, the epi-fluorescence light path is introduced and the various components are discussed in detail.
Donna J, Webb, Claire M, Brown
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Correlative stochastic optical reconstruction microscopy and electron microscopy. [PDF]
Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure.
Doory Kim +5 more
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
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