Results 21 to 30 of about 454,367 (198)

Deep-learning-based methods for super-resolution fluorescence microscopy

open access: yesJournal of Innovative Optical Health Sciences, 2023
The algorithm used for reconstruction or resolution enhancement is one of the factors affecting the quality of super-resolution images obtained by fluorescence microscopy. Deep-learning-based algorithms have achieved state-of-the-art performance in super-
Jianhui Liao   +3 more
doaj   +1 more source

A statistical resolution measure of fluorescence microscopy with finite photons [PDF]

open access: yes, 2023
First discovered by Ernest Abbe in 1873, the resolution limit of a far-field microscope is considered determined by the numerical aperture and wavelength of light, approximately $\lambda$/2NA. With the advent of modern fluorescence microscopy and nanoscopy methods over the last century, it is recognized that Abbe's resolution definition alone could not
arxiv   +1 more source

Two-photon laser scanning fluorescence microscopy using photonic crystal fibre [PDF]

open access: yes, 2004
We report the application of a simple yet powerful modular pulse compression system, based on photonic crystal fibres which improves upon incumbent twophoton laser scanning fluorescence microscopy techniques.
Birks   +13 more
core   +1 more source

Beyond Range: Innovating Fluorescence Microscopy

open access: yesRemote Sensing, 2012
Time-of-Flight (ToF) technologies are developed mainly for range estimations in industrial applications or consumer products. Recently, it was realized that ToF sensors could also be used for the detection of fluorescence and of the minute changes in the
Alessandro Esposito
doaj   +1 more source

Scanless multitarget-matching multiphoton excitation fluorescence microscopy [PDF]

open access: yesJournal of Innovative Optical Health Sciences, 2018
Using the combination of a reflective blazed grating and a reflective phase-only diffractive spatial light modulator (SLM), scanless multitarget-matching multiphoton excitation fluorescence microscopy (SMTM-MPM) was achieved.
Junpeng Qiu   +4 more
doaj   +1 more source

Multiple airy beams light-sheet fluorescence microscopy

open access: yesFrontiers in Physics, 2022
Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu   +9 more
doaj   +1 more source

J Fluorescence [PDF]

open access: yes, 2005
The scope of this paper is to illustrate the need for an improved quality assurance in fluorometry. For this purpose, instrumental sources of error and their influences on the reliability and comparability of fluorescence data are highlighted for ...
Ebert, P.   +7 more
core   +2 more sources

Evaluating performance in three-dimensional fluorescence microscopy [PDF]

open access: yes, 2007
In biological fluorescence microscopy, image contrast is often degraded by a high background arising from out of focus regions of the specimen. This background can be greatly reduced or eliminated by several modes of thick specimen microscopy, including ...
Chong F.K.   +6 more
core   +3 more sources

Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes

open access: yesFrontiers in Cell and Developmental Biology, 2022
Intracellular processes depend on a strict spatial and temporal organization of proteins and organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is crucial in understanding cellular physiology.
Sergey V. Loginov   +9 more
doaj   +1 more source

Recurrent neural network-based volumetric fluorescence microscopy

open access: yesLight: Science & Applications, 2021
Volumetric imaging of samples using fluorescence microscopy plays an important role in various fields including physical, medical and life sciences. Here we report a deep learning-based volumetric image inference framework that uses 2D images that are ...
Luzhe Huang   +4 more
doaj   +1 more source

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