Fast image reconstruction for fluorescence microscopy
AIP Advances, 2012 Real-time image reconstruction is essential for improving the temporal resolution of fluorescence microscopy. A number of unavoidable processes such as, optical aberration, noise and scattering degrade image quality, thereby making image reconstruction ...
Mahesh Ravi Varma +2 more
doaj +1 more source
Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations
Cells, 2021 Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell ...
Shane M. Hickey +12 more
doaj +1 more source
Performance of LED-Based Fluorescence Microscopy to Diagnose Tuberculosis in a Peripheral Health Centre in Nairobi. [PDF]
, 2011 Sputum microscopy is the only tuberculosis (TB) diagnostic available at peripheral levels of care in resource limited countries. Its sensitivity is low, particularly in high HIV prevalence settings. Fluorescence microscopy (FM) can improve performance of
A Cambanis +33 more
core +5 more sources
ixFLIM: Interferometric Excitation Fluorescence Lifetime Imaging Microscopy [PDF]
arXiv, 2023 Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an extension of FLIM by interferometric measurement of fluorescence excitation spectra.
arxiv
Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy [PDF]
Opt. Express 9, 1177-1189 (2018), 2019 We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with simultaneous 2-D fluorescence microscopy.
arxiv +1 more source
Fluorescence radial fluctuation enables two-photon super-resolution microscopy
Frontiers in Cellular Neuroscience, 2023 Despite recent improvements in microscopy, it is still difficult to apply super-resolution microscopy for deep imaging due to the deterioration of light convergence properties in thick specimens.
Motosuke Tsutsumi +7 more
doaj +1 more source
High performance, LED powered, waveguide based total internal reflection microscopy. [PDF]
, 2013 Total internal reflection fluorescence (TIRF) microscopy is a rapidly expanding optical technique with excellent surface sensitivity and limited background fluorescence. Commercially available TIRF systems are either objective based that employ expensive
Cohen, Daniel A +3 more
core +1 more source
Making tau amyloid models in vitro: a crucial and underestimated challenge
FEBS Letters, EarlyView.This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley +1 more source
Three-dimensional bi-functional refractive index and fluorescence microscopy (BRIEF) [PDF]
arXiv, 2020 Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical property that quantitatively relates to cell morphology, mass, and stiffness.
arxiv
Common-path multimodal optical microscopy [PDF]
, 2009 We have developed a common-path multimodal optical microscopy system that is capable of using a single optical source and a single camera to image amplitude, phase, and fluorescence features of a biological specimen.
Kothapalli, Sri-Rajasekhar +4 more
core +1 more source