Homologous Recombination under the Single-Molecule Fluorescence Microscope [PDF]
, 2019 Homologous recombination (HR) is a complex biological process and is central to meiosis and for repair of DNA double-strand breaks. Although the HR process has been the subject of intensive study for more than three decades, the complex protein–protein ...
Dhakal, Soma, Gibbs, Dalton R.
core +2 more sources
Polarization effects on fluorescence emission of zebrafish neurons using light-sheet microscopy [PDF]
arXiv, 2022 Light-sheet fluorescence microscopy (LSFM) makes use of a thin plane of light to optically section and image transparent tissues or organisms {\it{in vivo}}, which has the advantages of fast imaging speed and low phototoxicity. In this paper, we have employed light-sheet microscopy to investigate the polarization effects on fluorescence emission of ...
arxiv
Quantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera [PDF]
Journal of Biomedical Optics Vo.8, 362 (July 2003), 2003 Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. We review here the recent developments in multiphoton FLIM methods and also present a description of a novel multiphoton FLIM system using a ...
arxiv +1 more source
miR-203 drives breast cancer cell differentiation
Breast Cancer Research, 2023 A hallmark of many malignant tumors is dedifferentiated (immature) cells bearing slight or no resemblance to the normal cells from which the cancer originated.
Nuria G. Martínez-Illescas +20 more
doaj +1 more source
Epi-Fluorescence Microscopy [PDF]
, 2012 Epi-fluorescence microscopy is available in most life sciences research laboratories, and when optimized can be a central laboratory tool. In this chapter, the epi-fluorescence light path is introduced and the various components are discussed in detail.
Donna J. Webb, Claire M. Brown
openaire +3 more sources
In vitro reconstitution of microtubule dynamics and severing imaged by label-free interference reflection microscopy [PDF]
arXiv, 2021 The dynamic architecture of the microtubule cytoskeleton is crucial for cell division, motility and morphogenesis. The dynamic properties of microtubules - growth, shrinkage, nucleation and severing - are regulated by an arsenal of microtubule-associated proteins (MAPs).
arxiv
3D Computational Cannula Fluorescence Microscopy enabled by Artificial Neural Networks [PDF]
, 2020 Computational Cannula Microscopy (CCM) is a high-resolution widefield fluorescence imaging approach deep inside tissue, which is minimally invasive. Rather than using conventional lenses, a surgical cannula acts as a lightpipe for both excitation and fluorescence emission, where computational methods are used for image visualization.
arxiv +1 more source
Blind fluorescence structured illumination microscopy: A new reconstruction strategy [PDF]
, 2016 In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint.
Allain, M. +6 more
core +4 more sources
Super-resolution fluorescence microscopy by stepwise optical saturation [PDF]
Biomed. Opt. Express 9, 1613-1629 (2018), 2018 Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal application of super-resolution microscopy is not feasible.
arxiv +1 more source
Efficient non-degenerate two-photon excitation for fluorescence microscopy [PDF]
, 2019 Non-degenerate two-photon excitation (ND-TPE) has been explored in two-photon excitation microscopy. However, a systematic study of the efficiency of ND-TPE to guide the selection of fluorophore excitation wavelengths is missing. We measured the relative
Adams, Stephen R. +12 more
core +1 more source