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A Label-Free Fluorescence Polarization for Protein Detection Based on Albumin Nanoparticles [PDF]

open access: goldChemosensors
Human serum albumin (HSA), an endogenous protein, was employed in the synthesis of nanoparticles. The treatment of an HSA solution with ethanol and glutaraldehyde resulted in the formation of human serum albumin nanoparticles (HSA NPs), which exhibited a
Yunshu Wang   +5 more
doaj   +2 more sources

Application of the Chitooligosaccharides and Fluorescence Polarization Technique for the Assay of Active Lysozyme in Hen Egg White [PDF]

open access: yesBiomolecules
This study describes the applicability of the fluorescence polarization assay (FPA) based on the use of FITC-labeled oligosaccharide tracers of defined structure for the measurement of active lysozyme in hen egg white.
Liliya I. Mukhametova   +8 more
doaj   +2 more sources

Improving the Detection Accuracy of Subsurface Damage in Optical Materials by Exploiting the Fluorescence Polarization Properties of Quantum Dots [PDF]

open access: yesNanomaterials
Optical materials are widely used in large optical systems such as lithography machines and astronomical telescopes. However, optical materials inevitably produce subsurface damage (SSD) during lapping and polishing processes, degrading the laser damage ...
Yana Cui   +4 more
doaj   +2 more sources

Hanle Detection for Optical Clocks [PDF]

open access: yesThe Scientific World Journal, 2015
Considering the strong inhomogeneous spatial polarization and intensity distribution of spontaneous decay fluorescence due to the Hanle effect, we propose and demonstrate a universe Hanle detection configuration of electron-shelving method for optical ...
Xiaogang Zhang   +6 more
doaj   +4 more sources

Study of Lysozyme Activity in Bird Egg Whites by Fluorescence Polarization Assay Using Chitooligosaccharide Tracer [PDF]

open access: yesFoods
The storage duration and hatchability of eggs largely depend on the lysozyme content in egg whites; therefore, determining the lysozyme status is important for characterizing their quality.
Liliya I. Mukhametova   +6 more
doaj   +2 more sources

Development of a receptor based signal amplified fluorescence polarization assay for multi-detection of 35 sulfonamides in pork [PDF]

open access: yesFood Chemistry: X, 2023
With the increasing focus on food security, a screening method with high-throughput, ultra-sensitivity, and user-friendly operation is urgently needed for monitoring of sulfonamides residues in animal-derived foods.
Tong He   +5 more
doaj   +2 more sources

OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images. [PDF]

open access: yesPLoS Computational Biology
Most essential cellular functions are performed by proteins assembled into larger complexes. Fluorescence Polarization Microscopy (FPM) is a powerful technique that goes beyond traditional imaging methods by allowing researchers to measure not only the ...
William F Dean   +3 more
doaj   +2 more sources

Fluorescence Polarization Immunoassay for Rapid, Sensitive Detection of the Herbicide 2,4-Dichlorophenoxyacetic Acid in Juice and Water Samples [PDF]

open access: yesBiosensors
2,4-Dichlorophenoxyacetic acid (2,4-D) is one of the popular herbicides that is widely used in agriculture and can be found in food and water. A rapid and sensitive fluorescence polarization immunoassay (FPIA) was proposed for the detection of 2,4-D in ...
Liliya I. Mukhametova   +6 more
doaj   +2 more sources

A Fluorescence-Polarization-Based Lipopolysaccharide–Caspase-4 Interaction Assay for the Development of Inhibitors [PDF]

open access: yesMolecules, 2022
Recognition of intracellular lipopolysaccharide (LPS) by Caspase-4 (Casp-4) is critical for host defense against Gram-negative pathogens. LPS binds to the N-terminal caspase activation and recruitment domain (CARD) of procaspase-4, leading to auto ...
Jinsu An   +3 more
doaj   +2 more sources

A High-Throughput Fluorescence Polarization-Based Assay for the SH2 Domain of STAT4 [PDF]

open access: yesMethods and Protocols, 2022
The signal transducer and activation of transcription (STAT) proteins are a family of Src homology 2 (SH2) domain-containing transcription factors. The family member STAT4 is a mediator of IL-12 signalling and has been implicated in the pathogenesis of ...
Angela Berg   +4 more
doaj   +2 more sources

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