Results 1 to 10 of about 70 (69)
Fluorescence polarization of green fluorescence protein [PDF]
Proceedings of the National Academy of Sciences, 2002 We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea .
Shinya, Inoué +4 more
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Fluorescence polarization immunoassay of colchicine [PDF]
Journal of Pharmaceutical and Biomedical Analysis, 2018 In this study, a fluorescence polarization immunoassay (FPIA) technique was developed to determine colchicine (COL), an alkaloid of noxious plants of the order Liliales that is used in a number of medications to treat gout. An optimal combination of the polyclonal antibody and the antigen labelled with fluorescein isothiocyanate (FITC) was selected ...
Elena A. Zvereva +5 more
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Polarized fluorescent nanospheres
Optics Express, 2010 Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study.
Rafal, Luchowski +6 more
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Fluorescence polarization immunoassay for zidovudine [PDF]
Antimicrobial Agents and Chemotherapy, 1989 We report a fluorescence polarization immunoassay (FPIA) for zidovudine (azidothymidine; Retrovir). This assay is accurate and specific over the clinically relevant range of zidovudine concentrations in serum (from 1 to 1,250 ng/ml; from 0.004 to 4.8 microM) and is unaffected by potentially interfering compounds in the sera of patients with renal or ...
G G, Granich +2 more
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A fluorescence polarization flow cytometer [PDF]
Cytometry, 1980 AbstractThe effect of mitogens on the degree of polarization of the fluorescence from viable human lymphocytes, labeled with fluorescein, have been reported to be different in cells from subjects with malignant disease as compared with those from healthy donors.
J P, Keene, B W, Hodgson
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Polarization effects on fluorescence measurements [PDF]
Journal of Research of the National Bureau of Standards Section A: Physics and Chemistry, 1975 Polarization effects on fluorescence measurements are a function of four independent variables. The first is F, the polarization ratio of the exciting light which reaches the sample. The second is r, the emission anisotropy of the sample, which is the polarization "response" of the sample to plane polarized exciting light.
E D, Cehelnik +2 more
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Fluorescence Polarization Immunoassay of Progesterone.
Biological and Pharmaceutical Bulletin, 1997 A homogeneous fluorescence polarization immunoassay (FPIA) was developed to measure levels of progesterone in urine using a TDx analyzer in photocheck mode (Abbott Labs). Two tracers of ethylenediamine fluorescein thiocarbamyl (EDF) were employed; one was synthesized from 11 alpha-hydroxyhemisuccinate progesterone (Prog-11OH-HS) and the other was ...
M J, Choi +4 more
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Fluorescence Polarization-Based Bioassays: New Horizons [PDF]
Sensors, 2020 Fluorescence polarization holds considerable promise for bioanalytical systems because it allows the detection of selective interactions in real time and a choice of fluorophores, the detection of which the biosample matrix does not influence; thus, their choice simplifies and accelerates the preparation of samples.
Olga D. Hendrickson +4 more
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Fluorescence nanoscopy by polarization modulation and polarization angle narrowing [PDF]
Nature Methods, 2014 When excited with rotating linear polarized light, differently oriented fluorescent dyes emit periodic signals peaking at different times. We show that measurement of the average orientation of fluorescent dyes attached to rigid sample structures mapped to regularly defined (50 nm)(2) image nanoareas can provide subdiffraction resolution (super ...
Hafi, N. +10 more
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Biophysical Journal, 1994 The time-resolved anisotropy produced in polarized fluorescence photobleaching recovery experiments has been successfully used to measure rotational correlation times in a variety of biological systems, however the magnitudes of the reported initial anisotropies have been much lower than the theoretically predicted maximum values.
Hellen, E.H., Burghardt, T.P.
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