Results 21 to 30 of about 276,497 (319)

Polarized fluorescent nanospheres

open access: yesOptics Express, 2010
Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study.
Rafal, Luchowski   +6 more
openaire   +2 more sources

Detection of the four major human herpesviruses simultaneously in whole blood and cerebrospinal fluid samples by the fluorescence polarization assay [PDF]

open access: yes, 2010
SummaryObjectivesHerpes simplex virus type 1/2 (HSV-1/-2), cytomegalovirus (CMV), and Epstein–Barr virus (EBV) correlate strongly with infections of the central nervous system.
Ding, Li   +6 more
core   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

open access: yes, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe   +295 more
core   +2 more sources

Saturation effects in polarized fluorescence photobleaching recovery and steady state fluorescence polarization

open access: yesBiophysical Journal, 1994
The time-resolved anisotropy produced in polarized fluorescence photobleaching recovery experiments has been successfully used to measure rotational correlation times in a variety of biological systems, however the magnitudes of the reported initial anisotropies have been much lower than the theoretically predicted maximum values.
Edward H. Hellen, Thomas P. Burghardt
openaire   +3 more sources

A fluorescence polarization flow cytometer [PDF]

open access: yesCytometry, 1980
AbstractThe effect of mitogens on the degree of polarization of the fluorescence from viable human lymphocytes, labeled with fluorescein, have been reported to be different in cells from subjects with malignant disease as compared with those from healthy donors.
B. W. Hodgson, J. P. Keene
openaire   +3 more sources

Nonlinear optical techniques for improved data capture in fluorescence microscopy and imaging [PDF]

open access: yes, 2010
Multiphoton fluorescence microscopy is now a well-established technique, currently attracting much interest across all fields of biophysics - especially with regard to enhanced focal resolution.
Andrews, DL   +2 more
core   +1 more source

Light emission from dye-doped cholesteric liquid crystals at oblique angles: simulation and experiment [PDF]

open access: yes, 2012
Dye-doped cholesteric liquid crystals with a helical pitch of the order of a wavelength have a strong effect on the fluorescence properties of dye molecules. This is a promising system for realizing tunable lasers at low cost. We apply a plane wave model
Beeckman, Jeroen   +3 more
core   +1 more source

Dynamics of Fluorescence Polarization in Macromolecules [PDF]

open access: yesProceedings of the National Academy of Sciences, 1972
Reexamination of the theory of fluorescence time dependence owing to rotational diffusion of rigid macromolecules reveals deficiencies or hidden restrictions in each of the previous treatments. The correct master equation has five exponential decay terms, with preexponential factors that depend upon the diffusion constants and, in a completely ...
Gregorio Weber   +2 more
openaire   +3 more sources

The analysis of fluorophore orientation by multiphoton fluorescence microscopy [PDF]

open access: yes, 2010
The accessibility of tunable, ultrafast laser sources has spurred the development and wide application of specialized microscopy techniques based on chromophore fluorescence following two- and three-photon absorption.
Andrews, D.L., Leeder, J.M.
core   +1 more source

Polarization effects on fluorescence measurements [PDF]

open access: yesJournal of Research of the National Bureau of Standards Section A: Physics and Chemistry, 1975
Polarization effects on fluorescence measurements are a function of four independent variables. The first is F, the polarization ratio of the exciting light which reaches the sample. The second is r, the emission anisotropy of the sample, which is the polarization "response" of the sample to plane polarized exciting light.
R.A. Velapoldi   +2 more
openaire   +3 more sources

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