Results 21 to 30 of about 211,115 (326)

Fluorescence polarization of green fluorescence protein [PDF]

Proceedings of the National Academy of Sciences, 2002
We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea .
Shinya, Inoué, Osamu, Shimomura, Makoto, Goda, Mykhailo, Shribak, P T, Tran   +4 more
openaire   +3 more sources

Hyperdimensional Imaging Contrast Using an Optical Fiber

Sensors, 2021
Fluorescence properties of a molecule can be used to study the structural and functional nature of biological processes. Physical properties, including fluorescence lifetime, emission spectrum, emission polarization, and others, help researchers probe a ...
Jenu V. Chacko, Han Nim Lee, Wenxin Wu, Marisa S. Otegui, Kevin W. Eliceiri   +4 more
doaj   +1 more source

Fluorescence polarization immunoassay of colchicine [PDF]

Journal of Pharmaceutical and Biomedical Analysis, 2018
In this study, a fluorescence polarization immunoassay (FPIA) technique was developed to determine colchicine (COL), an alkaloid of noxious plants of the order Liliales that is used in a number of medications to treat gout. An optimal combination of the polyclonal antibody and the antigen labelled with fluorescein isothiocyanate (FITC) was selected ...
Elena A. Zvereva, Anatoly V. Zherdev, Andey A. Formanovsky, Ramadan A. Abuknesha, Sergei A. Eremin, Boris B. Dzantiev   +5 more
openaire   +5 more sources

Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences

Computational and Structural Biotechnology Journal, 2020
Fluorescence polarization microscopy (FPM) analyzes both intensity and orientation of fluorescence dipole, and reflects the structural specificity of target molecules. It has become an important tool for studying protein organization, orientational order,
Long Chen, Xingye Chen, Xusan Yang, Chao He, Miaoyan Wang, Peng Xi, Juntao Gao   +6 more
doaj   +1 more source

Polarized fluorescent nanospheres

Optics Express, 2010
Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study.
Rafal, Luchowski, Zygmunt, Gryczynski, Zeno, Földes-Papp, Aaron, Chang, Julian, Borejdo, Pabak, Sarkar, Ignacy, Gryczynski   +6 more
openaire   +2 more sources

Fluorescence polarization immunoassay for zidovudine [PDF]

Antimicrobial Agents and Chemotherapy, 1989
We report a fluorescence polarization immunoassay (FPIA) for zidovudine (azidothymidine; Retrovir). This assay is accurate and specific over the clinically relevant range of zidovudine concentrations in serum (from 1 to 1,250 ng/ml; from 0.004 to 4.8 microM) and is unaffected by potentially interfering compounds in the sera of patients with renal or ...
G G, Granich, M R, Eveland, D J, Krogstad   +2 more
openaire   +2 more sources

Fluorescence Polarization for Monitoring Ribozyme Reactions in Real Time

BioTechniques, 2000
Fluorescence polarization has been used recently to monitor diverse macromolecular interactions. In this report, the application of fluorescence polarization has been extended to monitor ribozyme reactions in real time.
K.K. Singh, T. Rücker, A. Hanne, R. Parwaresch, G. Krupp   +4 more
doaj   +1 more source

Temperature measurement in microchannel liquid flow by measuring fluorescence polarization

Nihon Kikai Gakkai ronbunshu, 2017
A fluid temperature measurement technique based on fluorescence polarization is developed and applied to measure the two-dimensional temperature distributions in microchannel.
Atsushi SUZUKI, Kazuya TATSUMI, Satoshi HORII, Reiko KURIYAMA, Kazuyoshi NAKABE   +4 more
doaj   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe, Abbe, Ai, Airy, Alivisatos, Alivisatos, Ando, Aoki, Auksorius, Auzel, Auzel, Axelrod, Axelrod, Bacskai, Bader, Bader, Balse, Barroso, Bastiaens, Bates, Beaudouin, Beaurepaire, Becker, Benninger, Berezin, Berney, Betzig, Bianchini, Biju, Binnemans, Borst, Brakemann, Bretschneider, Bunt, Campagnola, Campagnola, Campbell, Cantor, Carrero, Chalfie, Chalfie, Chatterjee, Chen, Chen, Clapp, Clapp, Clapp, Cole, Coons, Dahan, Dedecker, Del Pozo, Deng, Denk, Diaspro, Diaspro, Dickson, Digman, Drobizhev, Drummen, Dundr, Dundr, Dunn, Dunn, Dunn, Durr, Dyba, Ehrlicher, Einstein, Elangovan, Elder, Ellenberg, Elsner, Feige, Fereidouni, Festenstein, Frommer, Förster, Förster, Gamelin, Garcia-Mira, Garcia-Parajo, Gerlich, Giese, Giordano, Goodson, Gopich, Gordon, Gregor P. C. Drummen, Grotjohann, Gu, Gunnlaugsson, Gurskaya, Gustafsson, Göppert-Mayer, Ha, Haugland, Heimstädt, Hellen C. Ishikawa-Ankerhold, Hemmila, Hiller, Hitakomate, Hofkens, Hofmann, Houtsmuller, Houtsmuller, Howell, Hu, Hu, Huang, Ishihama, Jacobson, Jares-Erijman, Jiang, Johannes, Johnson, Jonkman, Jose, Jovin, Jovin, Kaiser, Kambara, Kapustina, Karasawa, Kardash, Kasha, Kawashima, Kenworthy, Kerppola, Kerppola, Kerppola, Kerppola, Kimura, Kinoshita, Klonis, Kner, Knoblauch, Konig, Koppel, Koster, Kota, Koushik, Kremers, Kremers, Kuningas, Kuningas, Kuningas, Laforge, Lakowicz, Lakowicz, Larson, Lee, Lichtman, Liebman, Lippincott-Schwartz, Liu, Llères, Loura, Lukyanov, Lundin, Mansfield, Mathew-Fenn, Mattheyses, Matz, Medintz, Mills, Mitchell, Miyawaki, Miyawaki, Moerner, Moneron, Monetta, Mueller, Mueller, Nagai, Nehls, Ormö, Paddock, Parsons, Patterson, Patterson, Patterson, Pawley, Pawley, Persechini, Peters, Phair, Phair, Phillips, Piston, Piston, Planck, Pockwinse, Politz, Poo, Puliti, Rajaram, Rantanen, Reinhard, Reits, Reymann, Richard Ankerhold, Richards, Riven, Rizzo, Rizzo, Robinson, Roduner, Rotblat, Roy, Rust, Sakon, Sbalzarini, Schawlow, Schönle, Seabrooke, Seah, Selvin, Shaner, Shav-Tal, Shaw, Shchyolkina, Shimomura, Shimomura, Shroff, Shu, Shyu, Smith, So, Solon, Soukka, Soumpasis, Sprague, Sprague, Stasevich, Stelzer, Stepanenko, Stokes, Stryer, Stryer, Subach, Sun, Suyver, Swaminathan, Swift, Sönnichsen, Tagawa, Taylor, Thaler, Tian, Tian, Tinnefeld, Tisler, Tocanne, Tomosugi, Tripathi, Tsien, Tsien, Uphoff, Valentin, van Driel, Van Munster, Van Munster, van Munster, van Rheenen, van Royen, van Royen, van Royen, Varghese, Venetta, Vogelsang, von Wichert, Vosch, Wallrabe, Wang, Wang, Wang, Wang, Wang, Wang, Welch, White, Williams, Willig, Wouters, Wouters, Wouters, Wouters, Wright, Xia, Yang, Youvan, Zhang, Zhang, Zimmer, Zipfel   +295 more
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A molecular theory for two-photon and three-photon fluorescence polarization [PDF]

, 2011
In the analysis of molecular structure and local order in heterogeneous samples, multiphoton excitation of fluorescence affords chemically specific information and high-resolution imaging. This report presents the results of an investigation that secures
Craig D. P., D. L. Andrews, Healy W. P., J. M. Leeder, Kawski A., Loudon R., Power E. A., Valeur B.   +7 more
core   +1 more source