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Polarized fluorescent nanospheres
Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study.
Rafal, Luchowski+6 more
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Detection of the four major human herpesviruses simultaneously in whole blood and cerebrospinal fluid samples by the fluorescence polarization assay [PDF]
SummaryObjectivesHerpes simplex virus type 1/2 (HSV-1/-2), cytomegalovirus (CMV), and Epstein–Barr virus (EBV) correlate strongly with infections of the central nervous system.
Ding, Li+6 more
core +1 more source
Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe+295 more
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The time-resolved anisotropy produced in polarized fluorescence photobleaching recovery experiments has been successfully used to measure rotational correlation times in a variety of biological systems, however the magnitudes of the reported initial anisotropies have been much lower than the theoretically predicted maximum values.
Edward H. Hellen, Thomas P. Burghardt
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A fluorescence polarization flow cytometer [PDF]
AbstractThe effect of mitogens on the degree of polarization of the fluorescence from viable human lymphocytes, labeled with fluorescein, have been reported to be different in cells from subjects with malignant disease as compared with those from healthy donors.
B. W. Hodgson, J. P. Keene
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Nonlinear optical techniques for improved data capture in fluorescence microscopy and imaging [PDF]
Multiphoton fluorescence microscopy is now a well-established technique, currently attracting much interest across all fields of biophysics - especially with regard to enhanced focal resolution.
Andrews, DL+2 more
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Light emission from dye-doped cholesteric liquid crystals at oblique angles: simulation and experiment [PDF]
Dye-doped cholesteric liquid crystals with a helical pitch of the order of a wavelength have a strong effect on the fluorescence properties of dye molecules. This is a promising system for realizing tunable lasers at low cost. We apply a plane wave model
Beeckman, Jeroen+3 more
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Dynamics of Fluorescence Polarization in Macromolecules [PDF]
Reexamination of the theory of fluorescence time dependence owing to rotational diffusion of rigid macromolecules reveals deficiencies or hidden restrictions in each of the previous treatments. The correct master equation has five exponential decay terms, with preexponential factors that depend upon the diffusion constants and, in a completely ...
Gregorio Weber+2 more
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The analysis of fluorophore orientation by multiphoton fluorescence microscopy [PDF]
The accessibility of tunable, ultrafast laser sources has spurred the development and wide application of specialized microscopy techniques based on chromophore fluorescence following two- and three-photon absorption.
Andrews, D.L., Leeder, J.M.
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Polarization effects on fluorescence measurements [PDF]
Polarization effects on fluorescence measurements are a function of four independent variables. The first is F, the polarization ratio of the exciting light which reaches the sample. The second is r, the emission anisotropy of the sample, which is the polarization "response" of the sample to plane polarized exciting light.
R.A. Velapoldi+2 more
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