Results 151 to 160 of about 40,790 (312)
Advanced Science, EarlyView.Synaptic vesicle (SV) motion and exocytosis dynamics in the presynaptic terminals of live neurons are intimately linked to synaptic transmission during neuronal activity. In this work, it is revealed that, during electrical stimulation, SVs located farther from their fusion sites exhibit greater straightness and experience larger mean forces directed ...
Gyunam Park +7 more
wiley +1 more source
Advanced Science, EarlyView.A cell‐mimetic hydrogel compartment is proposed to enhance the efficiency of enzymatic reactions. The naturally porous structure of a crowded hydrogel can simulate the intracellular microenvironment, promoting the aggregation of biomolecules and activity of DNA polymerase through excluded volume effects and compartmentalization.
Fangbin Xiao +3 more
wiley +1 more source
Insect I‐Type Lysozymes Function as Antiviral Proteases by Forming Biomolecular Condensates
Advanced Science, EarlyView.Upon rice viral infection, the insect vector activates the Toll–MyD88–Dorsal signaling cascade, inducing i‐type lysozyme (Lyz‐I1) expression. Lyz‐I1 functions as an antiviral protease through its conserved catalytic dyad Glu/Asp (E/D), mediating cleavage of viral proteins at specific Lys (K) residues.
Yu Du +5 more
wiley +1 more source
Microcondensate‐Mediated Intracellular Infusion of mRNA Across the Plasma Membrane
Angewandte Chemie, EarlyView.The delivery of mRNA without lipid nanoparticles remains challenging. FcB(L17E)3‐driven microcondensates with nucleic acids, aided by short ssDNA, enable coacervate formation, actin‐dependent cytosolic infusion, and in vivo protein expression, offering a potential platform for next‐generation mRNA therapeutics and vaccination.
Yoshimasa Kawaguchi +4 more
wiley +2 more sources
Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons [PDF]
, 2011 Chan-Ying Zheng +3 more
openalex +3 more sources
Advanced Science, EarlyView.Phase separation organizes cellular components, yet stabilizing such dynamic structures in artificial cells remains challenging. A photo‐crosslinking approach is presented that prolongs the lifetime of different structures in coacervate‐based artificial cells while maintaining dynamic formation.
Madelief A. M. Verwiel +4 more
wiley +1 more source
Angewandte Chemie, EarlyView.A membrane‐penetrating molecular device composed of triplex containing cholesterol‐modified acyclic L‐threoninol nucleic acid (L‐aTNA) was achieved. This device is applied to a photo‐triggered signal transduction system functioning on giant unilamellar vesicles. Abstract Membrane‐penetrating molecular devices are valuable biological tools.
Kaifu Liu +2 more
wiley +2 more sources
Advanced Science, EarlyView.Our research reveals that small‐molecule icariin (ICA) directly binds Ras‐GTPase‐activating protein SH3 domain‐binding protein 1 (G3BP1), inducing conformational change and oligomerization. This interaction confers neuroprotection by recruiting insulin‐like growth factor 2 mRNA‐binding protein 1 (IGF2BP1) and modulating N6‐methyladenosine (m6A ...
Ling Li +17 more
wiley +1 more source
Advanced Science, EarlyView.This study establishes an electric field‐induced directional migration model using fluorescence tension probes to visualize microfilament forces and intracellular osmotic pressure dynamics in the electrotactic migration of breast cancer cells. This model delineates how electromechanical interactions among membrane potential, ion channels, OP, traction ...
Ling Zhu +10 more
wiley +1 more source