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Optimizing Detection of Tissue Anisotropy by Fluorescence Recovery after Photobleaching
Bulletin of Mathematical Biology, 2006Fluorescence recovery after photobleaching (FRAP) has been widely used to measure fluid flow and diffusion in gels and tissues. It has not been widely used in detection of tissue anisotropy. This may be due to a lack of applicable theory, or due to inherent limitations of the method. We discuss theoretical aspects of the relationship between anisotropy
Lubkin, S. R., Wan, X.
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Confocal Fluorescence Recovery After Photobleaching of Green Fluorescent Protein in Solution
Journal of Fluorescence, 2006Fluorescence recovery after photobleaching (FRAP) is one of the most widely used approaches to quantitatively estimate diffusion characteristics of molecules in solution and cellular systems. In general, comparison of the diffusion times (t (1/2)) from a FRAP experiment provides qualitative estimates of diffusion rates.
Thomas J, Pucadyil +1 more
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Fluorescence Recovery after Photobleaching in Colloidal Science: Introduction and Application
ACS Biomaterials Science & Engineering, 2022FRAP (fluorescence recovery after photo bleaching) is a method for determining diffusion in material science. In industrial applications such as medications, foods, Medtech, hygiene, and textiles, the diffusion process has a substantial influence on the overall qualities of goods.
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Diffusion measurements in epidermal tissues with fluorescent recovery after photobleaching
Skin Research and Technology, 2008Background/purpose: Pressure ulcers are areas of soft tissue breakdown, resulting from sustained mechanical loading of the skin and underlying tissues. Measuring biochemical markers that are released upon mechanical loading by the epidermis seems a promising method for objective risk assessment of the development of pressure ulcers.
Cornelissen, L.H. +3 more
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Fluorescence Recovery after Photobleaching: Application to Nuclear Proteins
2005Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological research. The method was developed in the 1970s, when its biological application mainly focused on the mobility of fluorescently labelled constituents of the cell membrane.
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Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching
2007Cell migration is a complex cellular behavior that involves the controlled reorganization of the link between the actin cytoskeleton and the extracellular matrix. This mechanical connection is provided by transmembrane receptors of the integrin family. Integrins are heterodimeric receptors that undergo an allosteric switch when activated by external or
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Lipase Surface Diffusion Studied by Fluorescence Recovery after Photobleaching
Langmuir, 2005We have analyzed surface diffusion properties of a variant of Thermomyces lanuginosa lipase (TLL) on hydrophilic silica and silica methylated with dichlorodimethylsilane (DDS) or octadecyltrichlorosilane (OTS). For this study a novel method for analysis of diffusion on solid surfaces was developed.
Andreas W, Sonesson +3 more
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Diffusion Measurements by Fluorescence Recovery After Photobleaching
2001Fluorescence recovery after photobleaching (FRAP) is a classic technique for measurement of the translational diffusion of fluorophores and fluorescently labeled macromolecules. In spot photobleaching, a brief intense light pulse irreversibly bleaches fluorophores in a defined volume of a fluorescent sample. With an attenuated probe beam, the diffusion
Alan S. Verkman +2 more
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Measuring Immune Receptor Mobility by Fluorescence Recovery After Photobleaching
2011The coordinated effort of cells in the immune system relies heavily on surface receptor interactions. Immune receptor mobility provides vital information on the function and responses of immune cells, and these measurements shed light on their interactions with other membrane, cytosolic, and extracellular matrix proteins.
Kristen, Silver, Rene E, Harrison
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Fluorescence recovery after photobleaching reveals the biochemistry of nucleocytoplasmic exchange
Analytical and Bioanalytical Chemistry, 2012Fluorescence recovery after photobleaching (FRAP) can help unveil subtle dynamical and biochemical properties of intracellular components. A peculiar aspect of this method is that it is based on the change of optical properties only, whereas dynamics and biochemistry of the molecules of interest are not perturbed.
Bizzarri R +3 more
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