Aquaporin embedded suspended lipid bilayer on anodized alumina nanoporous substrates for studying stability and functionality: towards the development of a miniaturized water purifier. [PDF]
Nanoscale AdvKumari A +2 more
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Multimodal optical system for quantitative discrimination of bilirubin species. [PDF]
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Fluorescence Recovery After Photobleaching
Methods in Molecular Biology, 2011This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as
Alexandre F Carisey +2 more
exaly +6 more sources
Long-Term Fluorescence Recovery After Photobleaching (FRAP)
Methods in Molecular Biology, 2023Numerous models have been developed for the analysis of fluorescence recovery after photobleaching (FRAP), by which intracellular diffusion and turnover rate are quantitatively evaluated. FRAP analyses typically focus on such events that occur within several minutes, but to precisely evaluate a slow turnover rate of particularly actin stress fibers ...
Takumi Saito +2 more
exaly +3 more sources
Frequency Domain Analysis for Fluorescence Recovery after Photobleaching
Applied Spectroscopy, 2006Fourier transformation is evaluated as a means of improving precision in the analysis of fluorescence-recovery-after-photobleaching (FRAP) data. Simulations of FRAP data of 2 m points, where m is an integer, are Fourier transformed to obtain the frequency domain data.
Mary J Wirth
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Toward reducing uncertainty in Fluorescence Recovery After Photobleaching
2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), 2013We investigate the uncertainty associated with the Fluorescence Recovery After Photobleaching, FRAP, which is widely used in the determination of diffusion coefficient for bio molecules. The uncertainty of our FRAP technique stems from the measurement of the spot size and the half time.
Jeonghoon Lee +3 more
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Probing the dynamics of photosynthetic membranes with fluorescence recovery after photobleaching
Trends in Plant Science, 2002In the past few years, there has been remarkable progress in knowledge of the structures and organization of the protein complexes of photosynthetic membranes. However, static structures do not tell the whole story. Photosynthetic membranes, like other biological membranes, are dynamic systems.
Conrad W Mullineaux, Mary Sarcina
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Fluorescence recovery after photobleaching of suspensions of vacuoles
Biophysical Chemistry, 1996In this work we derive theoretical expressions for the FRAP measured on a liquid suspension of vacuoles labelled by a fluorescent probe bound to the surface membrane of these vacuoles. The bleaching laser beam creates an inhomogeneity in the surface concentration of the probe molecules.
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Fluorescence Recovery after Photobleaching as a Probe of Diffusion in Starch Systems
Biomacromolecules, 2006The diffusion coefficients of dextran probes of various molecular weights in starch solutions over a wide concentration range were carried out using fluorescent recovery after photobleaching (FRAP), combined with a confocal microscope and tracer probe diffusion.
Perry, P. A. +2 more
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