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Fluorescence Recovery After Photobleaching (FRAP) with a Focus on F‐actin
Current Protocols in Neuroscience, 2012AbstractIn this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein‐protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area.
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Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis, and Applications
2014A significant number of biological processes occur at, or involve cellular membranes, including; cell adhesion, migration, endocytosis, signal transduction, and many biochemical reactions involving membrane anchored scaffolds. Each process involves a complex arrangement of interacting molecules whose location in space and time influence the outcome of ...
Michael, Carnell +2 more
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Fluorescence Recovery After Photobleaching Studies of Lipid Rafts
2007Fluorescence recovery after photobleaching (FRAP) is a microscopy-based technique that can be used to ask how lipid rafts impact protein and lipid diffusion in cells. This chapter, describes how to perform FRAP measurements of putative raft and nonraft proteins and lipids using a confocal microscope.
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Peer Reviewed: Applications of Fluorescence Recovery after Photobleaching
Analytical Chemistry, 1997Determining the diffusion coefficients of fluorescent molecules provides details on molecular-scale structure.
John M. Kovaleski, Mary J. Wirth
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Fluorescence recovery after photobleaching
2021Favard, Cyril, Sibarita, Jean-Baptiste
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Fluorescence Recovery After Photobleaching
Imaging & Microscopy, 2006Sarah Burrows +3 more
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Fast Diffusion Characterization by Multiphoton Excited Fluorescence Recovery while Photobleaching
Analytical Chemistry, 2023Minghe Li +2 more
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Fluorescence Recovery After Photobleaching
2013Hendrik Deschout, Kevin Braeckmans
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