Anomalous photobleaching in fluorescence recovery after photobleaching measurements due to excitation saturation—a case study for fluorescein [PDF]
, 2006 Kevin Braeckmans +4 more
openalex +1 more source
Activatable smart contrast agents for photoacoustic imaging
Smart Molecules, EarlyView.This review highlights recent advances in smart activatable photoacoustic imaging (PAI) contrast agents, which dynamically modulate their optical properties in response to external stimuli or microenvironmental cues. It discusses their molecular design, activation mechanisms, biomedical applications, and future prospects for clinically tailored use ...
Donghyeon Oh +5 more
wiley +1 more source
Three-Dimensional Fluorescence Recovery after Photobleaching with the Confocal Scanning Laser Microscope [PDF]
, 2003 Kevin Braeckmans +4 more
openalex +1 more source
Self‐Driving Microscopes: AI Meets Super‐Resolution Microscopy
Small Methods, EarlyView.This review examines the use of machine learning to automate super‐resolution optical microscopy, enabling the microscope to autonomously make decisions on what, when, and how to image. By eliminating the need for human intervention, this approach has the potential to enhance the versatility and accessibility of super‐resolution microscopy.
Edward N. Ward +3 more
wiley +1 more source
Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy. [PDF]
Biophys Physicobiol, 2018 Kitamura A, Kinjo M.
europepmc +1 more source
Dual‐function, Reusable, and Flexible Thermal Interface for Kinetic Monitoring of In Vitro Bioassays
Small Methods, EarlyView.This work presents a reusable, dual‐function thermal interface for non‐contact, label‐free sensing and microwell‐independent temperature control in microplates within a single element. Real‐time monitoring of E. coli growth and the response to antibiotic treatment demonstrates the sensor's enhanced sensitivity in interface‐dominated processes compared ...
Daniel Nieder +4 more
wiley +1 more source
Small Methods, EarlyView.A new method to identify proteins involved in the uptake and intracellular trafficking of nanoparticles is presented. After nanoparticle uptake, cells are lysed and the organelles with nanoparticles are purified using fluorescence assisted cell sorting. Next, their composition is determined by proteomic analysis.
Hector Garcia Romeu, Anna Salvati
wiley +1 more source
Quantizing DNA Metallization for Site‐Defined Growth of Single Quantum Emitters
Small Structures, EarlyView.Predictive design rules guide DNA origami‐templated synthesis of atomically precise silver nanoclusters. Controlled nucleation yields single emitters with nanometer registration and reproducible fluorescence signatures, paving the way toward programable quantum photonic architectures and nanoscale device integration.
Swati Tanwar +6 more
wiley +1 more source
Fluorescence recovery after photobleaching investigation of protein transport and exchange in chromatographic media. [PDF]
J Chromatogr A, 2014 Traylor SJ +4 more
europepmc +1 more source