A Simple, Ultrastable, and Cost‐Effective Oxygen‐Scavenging System for Long‐Term DNA‐PAINT Imaging
Small, EarlyView.A simple, enzyme‐free, and cost‐effective oxygen scavenging system enhances DNA‐PAINT super‐resolution microscopy by increasing photostability, minimizing docking strand degradation, and enabling faster, high‐precision localizations. Its long‐term stability supports extended imaging, yielding improved spatial resolution and image quality.
Rebecca T. Perelman +2 more
wiley +1 more source
Volumetric Single‐Molecule Tracking Inside Subcellular Structures
Small, EarlyView.This correlative microscopy approach integrates single‐molecule light‐field microscopy (SMLFM) with widefield Fourier light‐field microscopy (FLFM) to enable 3D single‐molecule tracking within the organelles of living mammalian cells. Instantaneous volumetric segmentation enhances the sensitivity of molecular organization and diffusion measurements ...
Sam Daly +8 more
wiley +1 more source
Diffusional Mobility of Parvalbumin in Spiny Dendrites of Cerebellar Purkinje Neurons Quantified by Fluorescence Recovery after Photobleaching [PDF]
, 2003 Hartmut Schmidt +3 more
openalex +1 more source
Expanding The Applicability Of The Multi-photon Fluorescence Recovery After Photobleaching Technique In Vivo Using A New Convective Flow Model [PDF]
, 2009 Kelley D. Sullivan
openalex +1 more source
Activatable smart contrast agents for photoacoustic imaging
Smart Molecules, EarlyView.This review highlights recent advances in smart activatable photoacoustic imaging (PAI) contrast agents, which dynamically modulate their optical properties in response to external stimuli or microenvironmental cues. It discusses their molecular design, activation mechanisms, biomedical applications, and future prospects for clinically tailored use ...
Donghyeon Oh +5 more
wiley +1 more source
Dual‐function, Reusable, and Flexible Thermal Interface for Kinetic Monitoring of In Vitro Bioassays
Small Methods, EarlyView.This work presents a reusable, dual‐function thermal interface for non‐contact, label‐free sensing and microwell‐independent temperature control in microplates within a single element. Real‐time monitoring of E. coli growth and the response to antibiotic treatment demonstrates the sensor's enhanced sensitivity in interface‐dominated processes compared ...
Daniel Nieder +4 more
wiley +1 more source
Fluorescence recovery after photobleaching (FRAP) assays of ChHog1:Gfp mobility.
Rina Zuchman (409382) +6 more
openalex +1 more source
Parameter Identifiability of RNA Dynamics in PDE Transport Models of Fluorescence Recovery After Photobleaching [PDF]
Qiang Xu
openalex +1 more source
Small Methods, EarlyView.A new method to identify proteins involved in the uptake and intracellular trafficking of nanoparticles is presented. After nanoparticle uptake, cells are lysed and the organelles with nanoparticles are purified using fluorescence assisted cell sorting. Next, their composition is determined by proteomic analysis.
Hector Garcia Romeu, Anna Salvati
wiley +1 more source
Quantizing DNA Metallization for Site‐Defined Growth of Single Quantum Emitters
Small Structures, EarlyView.Predictive design rules guide DNA origami‐templated synthesis of atomically precise silver nanoclusters. Controlled nucleation yields single emitters with nanometer registration and reproducible fluorescence signatures, paving the way toward programable quantum photonic architectures and nanoscale device integration.
Swati Tanwar +6 more
wiley +1 more source