Results 61 to 70 of about 41,336 (313)

Fluorescence Recovery after Photobleaching in Ultrathin Polymer Films

Macromolecular Chemistry and Physics, 2017
Abstract Fluorescence recovery after photobleaching (FRAP) is a widely used technique to study the transport of molecules in biological systems. Recently, FRAP has been used to study molecular transport in polyelectrolyte multilayers (PEMs).
Praneet Prakash, Suman Pahal, Manoj Varma   +2 more
openaire   +2 more sources

Fluorescence Recovery after Photobleaching: The Case of Anomalous Diffusion [PDF]

Biophysical Journal, 2008
The method of FRAP (fluorescence recovery after photobleaching), which has been broadly used to measure lateral mobility of fluorescent-labeled molecules in cell membranes, is formulated here in terms of continuous time random walks (CTRWs), which offer both analytical expressions and a scheme for numerical simulations.
Lubelski, Ariel, Klafter, Joseph
openaire   +2 more sources

Calibrating evanescent-wave penetration depths for biological TIRF microscopy

, 2019
Roughly half of a cells proteins are located at or near the plasma membrane. In this restricted space the cell senses its environment, signals to its neighbors and ex-changes cargo through exo- and endocytotic mechanisms.
Becherer, Ute, Brunstein, Maia, Oheim, Martin, Salomon, Adi, Weissman, Adam   +4 more
core   +1 more source

Differences in protein mobility between pioneer versus follower growth cones [PDF]

, 2007
Navigating growth cones need to integrate, process and respond to guidance signals, requiring dynamic information transfer within and between different compartments.
Bak, Bovolenta, Bovolenta, Brown, Campbell, Cheutin, Chitnis, Dent, Festenstein, Flanagan, Forscher, Gallo, Higashijima, Klonis, Lippincott-Schwartz, Mastick, McConnell, Meng, Myers, Nehls, Phair, Popov, Solnica-Krezel, Tessier-Lavigne, White, Wilson   +25 more
core   +3 more sources

Sequence determinants of in cell condensate morphology, dynamics, and oligomerization as measured by number and brightness analysis

Cell Communication and Signaling, 2021
Background Biomolecular condensates are non-stoichiometric assemblies that are characterized by their capacity to spatially concentrate biomolecules and play a key role in cellular organization.
Ryan J. Emenecker, Alex S. Holehouse, Lucia C. Strader   +2 more
doaj   +1 more source

TIA1 Mutations in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia Promote Phase Separation and Alter Stress Granule Dynamics. [PDF]

, 2017
Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are age-related neurodegenerative disorders with shared genetic etiologies and overlapping clinical and pathological features. Here we studied a novel ALS/FTD family and identified the
Annu, Kavya, Baker, Matt, Bigio, Eileen, Boylan, Kevin B., Caselli, Richard J., Dickson, Dennis W., Finger, Elizabeth, Fryer, John D., Graff-Radford, Neill R., Hirsch-Reinshagen, Veronica, Hsiung, Ging-Yuek R., Kato, Masato, Keith, Julia, Kim, Hong Joo, Krieger, Charles, Kurti, Aishe, Lacomis, David, Lippa, Carol, Mackenzie, Ian R., Matchett, Billie J., Messing, James, Mesulam, Marsel, Mittag, Tanja, Murray, Melissa E., Nicholson, Alexandra M., Perkerson, Ralph B., Petersen, Ronald C., Petrucelli, Leonard, Pottier, Cyril, Purice, Maria D., Rademakers, Rosa, Rogaeva, Ekaterina, Roos, Raymond P., Sarkar, Mohona, Stewart, Heather, Taylor, J. Paul, Temirov, Jamshid, Weintraub, Sandra, Wszolek, Zbigniew K., Zinman, Lorne, Zivkovic, Sasha A., Züchner, Stephan   +41 more
core   +2 more sources

HURP localization in metaphase is the result of a multi-step process requiring its phosphorylation at Ser627 residue

Frontiers in Cell and Developmental Biology, 2023
Faithful chromosome segregation during cell division requires accurate mitotic spindle formation. As mitosis occurs rapidly within the cell cycle, the proteins involved in mitotic spindle assembly undergo rapid changes, including their interactions with ...
Stylianos Didaskalou, Christos Efstathiou, Christos Efstathiou, Sotirios Galtsidis, Ilοna Kesisova, Ilοna Kesisova, Aliaksandr Halavatyi, Tountzai Elmali, Avgi Tsolou, Andreas Girod, Maria Koffa   +10 more
doaj   +1 more source

Cytoplasmic electric fields and electroosmosis: possible solution for the paradoxes of the intracellular transport of biomolecules.

PLoS ONE, 2013
The objective of the paper is to show that electroosmotic flow might play an important role in the intracellular transport of biomolecules. The paper presents two mathematical models describing the role of electroosmosis in the transport of the ...
Victor P Andreev
doaj   +1 more source

A FRAP model to investigate reaction-diffusion of proteins within a bounded domain: a theoretical approach

, 2008
Temporally and spatially resolved measurements of protein transport inside cells provide important clues to the functional architecture and dynamics of biological systems. Fluorescence Recovery After Photobleaching (FRAP) technique has been used over the
Axelrod, Beaudouin, Blonk, Braeckmans, Braga, Braga, Carmo-Fonseca, Carrero, Carrero, Carrero, Carslaw, Crank, Dundr, Edidin, George D. Tsibidis, Hinow, Houtsmuller, Jacobson, Jacques, Jacques, Jorge Ripoll, Koppel, Lele, Lippincott-Schwartz, Meyvis, Peters, Peters, Phair, Phair, Reits, Soumpasis, Sprague, Sprague, Sprague, Tang, Tardy, Th’ng, Wedekind, Wedekind, Weiss, White   +40 more
core   +1 more source

Fluorescence recovery after photobleaching (FRAP) v1

, 2022
This protocol details methods of the FRAP analysis of LRRK2-induced liposome tubules in vitro
openaire   +1 more source