Results 61 to 70 of about 40,790 (312)

Cytoplasmic electric fields and electroosmosis: possible solution for the paradoxes of the intracellular transport of biomolecules.

PLoS ONE, 2013
The objective of the paper is to show that electroosmotic flow might play an important role in the intracellular transport of biomolecules. The paper presents two mathematical models describing the role of electroosmosis in the transport of the ...
Victor P Andreev
doaj   +1 more source

HURP localization in metaphase is the result of a multi-step process requiring its phosphorylation at Ser627 residue

Frontiers in Cell and Developmental Biology, 2023
Faithful chromosome segregation during cell division requires accurate mitotic spindle formation. As mitosis occurs rapidly within the cell cycle, the proteins involved in mitotic spindle assembly undergo rapid changes, including their interactions with ...
Stylianos Didaskalou, Christos Efstathiou, Christos Efstathiou, Sotirios Galtsidis, Ilοna Kesisova, Ilοna Kesisova, Aliaksandr Halavatyi, Tountzai Elmali, Avgi Tsolou, Andreas Girod, Maria Koffa   +10 more
doaj   +1 more source

A FRAP model to investigate reaction-diffusion of proteins within a bounded domain: a theoretical approach

, 2008
Temporally and spatially resolved measurements of protein transport inside cells provide important clues to the functional architecture and dynamics of biological systems. Fluorescence Recovery After Photobleaching (FRAP) technique has been used over the
Axelrod, Beaudouin, Blonk, Braeckmans, Braga, Braga, Carmo-Fonseca, Carrero, Carrero, Carrero, Carslaw, Crank, Dundr, Edidin, George D. Tsibidis, Hinow, Houtsmuller, Jacobson, Jacques, Jacques, Jorge Ripoll, Koppel, Lele, Lippincott-Schwartz, Meyvis, Peters, Peters, Phair, Phair, Reits, Soumpasis, Sprague, Sprague, Sprague, Tang, Tardy, Th’ng, Wedekind, Wedekind, Weiss, White   +40 more
core   +1 more source

Analysis of Diffusion of Ras2 in Saccharomyces cerevisiae Using Fluorescence Recovery after Photobleaching

, 2010
Binding, lateral diffusion and exchange are fundamental dynamic processes involved in protein association with cellular membranes. In this study, we developed numerical simulations of lateral diffusion and exchange of fluorophores in membranes with ...
Daniel A Beard, David A Mitchell, Ito H, Kalyan C Vinnakota, Landaw E M, LeVeque R J, Muller M, Robert J Deschenes, Sherman F, Tetsuro Wakatsuki, Van Der Rest M E   +10 more
core   +1 more source

A Method Improving the Accuracy of Fluorescence Recovery after Photobleaching Analysis [PDF]

Biophysical Journal, 2008
Fluorescence recovery after photobleaching has been an established technique of quantifying the mobility of molecular species in cells and cell membranes for more than 30 years. However, under nonideal experimental conditions, the current methods of analysis still suffer from occasional problems; for example, when the signal/noise ratio is low, when ...
Jönsson, Peter, Jonsson, Magnus P., Tegenfeldt, Jonas O., Höök, Fredrik   +3 more
openaire   +2 more sources

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Photoswitching Conduction in Framework Materials

Advanced Functional Materials, EarlyView.
This mini‐review summarizes recent advances in state‐of‐the‐art proton and electron conduction in framework materials that can be remotely and reversibly switched on and off by light. It discusses the various photoswitching conduction mechanisms and the strategies employed to enhance photoswitched conductivity.
Helmy Pacheco Hernandez, Stefan Hecht, Wolfgang Wenzel, Lars Heinke, Mariana Kozlowska   +4 more
wiley   +1 more source

Effects of a synthetic bioactive peptide on neurite growth and nerve growth factor release in chondroitin sulfate hydrogels. [PDF]

, 2011
Previous work has revealed robust dorsal root ganglia neurite growth in hydrogels of chondroitin sulfate. In the current work, it was determined whether addition of a synthetic bioactive peptide could augment neurite growth in these matrices via enhanced
Beier, Brooke L, Conovaloff, Aaron W, Irazoqui, Pedro P, Panitch, Alyssa   +3 more
core   +1 more source

Block Copolymer Microcapsules with Perpendicularly‐Perforated Ultrathin Membranes for Controlled Transmembrane Transport

Advanced Functional Materials, EarlyView.
Block copolymer microcapsules with ultrathin, perpendicularly perforated membranes are developed through double emulsions, initiated by the diffusion of water into single emulsions. Both shell thickness and channel diameter can be systematically tuned, offering precision in molecular sieving and significantly enhanced permeation rates.
Zhengping Tan, Wahyu Martumpal Hamonangan, Jinwoo Kim, Younghyeon Ahn, Jaeyoung Choi, Meng Xu, Sanghyuk Park, Jinseok Park, Shin‐Hyun Kim, Bumjoon J. Kim   +9 more
wiley   +1 more source

Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability

Nature Communications, 2016
Measuring the size distribution of nanomaterials in biological fluids is crucial to understand their properties in vivo. Here, the authors apply fluorescence recovery after photobleaching to measure protein aggregation in serum and to study permeability ...
Ranhua Xiong, Roosmarijn E. Vandenbroucke, Katleen Broos, Toon Brans, Elien Van Wonterghem, Claude Libert, Jo Demeester, Stefaan C. De Smedt, Kevin Braeckmans   +8 more
doaj   +1 more source