Results 41 to 50 of about 55,338 (292)
Fluorescence Recovery After Photobleaching (FRAP) in the Fission Yeast Nucleus
Bio-Protocol, 2013 We use fluorescence recovery after photobleaching (FRAP) to calculate the diffusion coefficient of GFP in the nucleoplasm of fission yeast. The FRAP method can be generally used to measure the mobility of proteins inside the cell or its organelles.
Petrina Delivani +3 more
doaj +1 more source
eLife, 2022 Synapses maintain both action potential-evoked and spontaneous neurotransmitter release; however, organization of these two forms of release within an individual synapse remains unclear.
Camille S Wang +3 more
doaj +1 more source
Dynamic L-type CaV1.2 channel trafficking facilitates CaV1.2 clustering and cooperative gating. [PDF]
, 2018 L-type CaV1.2 channels are key regulators of gene expression, cell excitability and muscle contraction. CaV1.2 channels organize in clusters throughout the plasma membrane.
Brust-Mascher, Ingrid +8 more
core +2 more sources
A pharmacological cocktail for arresting actin dynamics in living cells. [PDF]
, 2011 The actin cytoskeleton is regulated by factors that influence polymer assembly, disassembly, and network rearrangement. Drugs that inhibit these events have been used to test the role of actin dynamics in a wide range of cellular processes.
Peng, Grace E +2 more
core +2 more sources
Measurement of Junctional Protein Dynamics Using Fluorescence Recovery After Photobleaching (FRAP)
Bio-Protocol, 2013 Fluorescence Recovery After Photobleaching (FRAP) (Lippincott-Schwartz et al., 2003; Reits and Neefjes, 2001) was employed to determine dynamic properties of proteins localized at the ephitelial zonula adherens (ZA) (Kovacs et al., 2011; Otani et al ...
Rashmi Priya, Guillermo Gomez
doaj +1 more source
Viruses, 2021 HIV-1 infection is enhanced by cell–cell adhesions between infected and uninfected T cells called virological synapses (VS). VS are initiated by the interactions of cell-surface HIV-1 envelope glycoprotein (Env) and CD4 on target cells and act as sites ...
Lili Wang +5 more
doaj +1 more source
Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress [PDF]
, 2018 Mechanical stimuli play a key role in many cell functions such as proliferation, differentiation and migration. In the mammary gland, mechanical signals such as the distension of mammary epithelial cells due to udder filling are proposed to be directly ...
Bianchi, Micaela +5 more
core +1 more source
Biochimica et Biophysica Acta. Gene Regulatory Mechanisms, 2019 Cells respond to their environment via an intricate cellular signaling network, directing cell fate. Changes in cell fate are characterized by changes in gene transcription, dictated by (master) transcription factor activity.
K. Govindaraj +4 more
semanticscholar +1 more source
Length-dependent disassembly maintains four different flagellar lengths in Giardia. [PDF]
, 2019 With eight flagella of four different lengths, the parasitic protist Giardia is an ideal model to evaluate flagellar assembly and length regulation. To determine how four different flagellar lengths are maintained, we used live-cell quantitative imaging ...
Aurrecoechea +69 more
core +2 more sources
Probing the Molecular Mechanism of Viscoelastic Relaxation in Transient Networks
Gels, 2023 Hydrogels, which have polymer networks through supramolecular and reversible interactions, exhibit various mechanical responsibilities to its surroundings.
Shota Michida +2 more
doaj +1 more source