Results 61 to 70 of about 55,338 (292)

Mass Transfer in Mesoporous Microparticles Studied by Confocal Fluorescence Recovery after Photobleaching

Analytical Sciences, 2017
The intraparticle diffusion of a fluorescent dye in single microparticles in an aqueous solution was analyzed using fluorescence recovery after photobleaching, with a confocal fluorescence microscope.
Tatsumi Sato, Katsuya Hata, K. Nakatani
semanticscholar   +1 more source

A FRAP model to investigate reaction-diffusion of proteins within a bounded domain: a theoretical approach

, 2008
Temporally and spatially resolved measurements of protein transport inside cells provide important clues to the functional architecture and dynamics of biological systems. Fluorescence Recovery After Photobleaching (FRAP) technique has been used over the
Axelrod, Beaudouin, Blonk, Braeckmans, Braga, Braga, Carmo-Fonseca, Carrero, Carrero, Carrero, Carslaw, Crank, Dundr, Edidin, George D. Tsibidis, Hinow, Houtsmuller, Jacobson, Jacques, Jacques, Jorge Ripoll, Koppel, Lele, Lippincott-Schwartz, Meyvis, Peters, Peters, Phair, Phair, Reits, Soumpasis, Sprague, Sprague, Sprague, Tang, Tardy, Th’ng, Wedekind, Wedekind, Weiss, White   +40 more
core   +1 more source

Differences in protein mobility between pioneer versus follower growth cones [PDF]

, 2007
Navigating growth cones need to integrate, process and respond to guidance signals, requiring dynamic information transfer within and between different compartments.
Bak, Bovolenta, Bovolenta, Brown, Campbell, Cheutin, Chitnis, Dent, Festenstein, Flanagan, Forscher, Gallo, Higashijima, Klonis, Lippincott-Schwartz, Mastick, McConnell, Meng, Myers, Nehls, Phair, Popov, Solnica-Krezel, Tessier-Lavigne, White, Wilson   +25 more
core   +3 more sources

Sequence determinants of in cell condensate morphology, dynamics, and oligomerization as measured by number and brightness analysis

Cell Communication and Signaling, 2021
Background Biomolecular condensates are non-stoichiometric assemblies that are characterized by their capacity to spatially concentrate biomolecules and play a key role in cellular organization.
Ryan J. Emenecker, Alex S. Holehouse, Lucia C. Strader   +2 more
doaj   +1 more source

Tobacco WLIM1 is a novel F-actin binding protein involved in actin cytoskeleton remodeling [PDF]

, 2006
We used confocal microscopy and in vitro analyses to show that Nicotiana tabacum WLIM1, a LIM domain protein related to animal Cys-rich proteins, is a novel actin binding protein in plants.
Thomas, Clément, Hoffmann, Céline, Dieterle, Monika, Van Troys, Marleen, Ampe, Christophe, Steinmetz, André   +5 more
core   +2 more sources

Fluorescence recovery after photobleaching (FRAP) v1

, 2022
This protocol details methods of the FRAP analysis of LRRK2-induced liposome tubules in vitro
openaire   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Cytoplasmic electric fields and electroosmosis: possible solution for the paradoxes of the intracellular transport of biomolecules.

PLoS ONE, 2013
The objective of the paper is to show that electroosmotic flow might play an important role in the intracellular transport of biomolecules. The paper presents two mathematical models describing the role of electroosmosis in the transport of the ...
Victor P Andreev
doaj   +1 more source

HURP localization in metaphase is the result of a multi-step process requiring its phosphorylation at Ser627 residue

Frontiers in Cell and Developmental Biology, 2023
Faithful chromosome segregation during cell division requires accurate mitotic spindle formation. As mitosis occurs rapidly within the cell cycle, the proteins involved in mitotic spindle assembly undergo rapid changes, including their interactions with ...
Stylianos Didaskalou, Christos Efstathiou, Christos Efstathiou, Sotirios Galtsidis, Ilοna Kesisova, Ilοna Kesisova, Aliaksandr Halavatyi, Tountzai Elmali, Avgi Tsolou, Andreas Girod, Maria Koffa   +10 more
doaj   +1 more source

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching [PDF]

Journal of Visualized Experiments, 2010
Multi-fluorescence recovery after photobleaching is a microscopy technique used to measure the diffusion coefficient (or analogous transport parameters) of macromolecules, and can be applied to both in vitro and in vivo biological systems. Multi-fluorescence recovery after photobleaching is performed by photobleaching a region of interest within a ...
Kelley D, Sullivan, Edward B, Brown
openaire   +2 more sources