Advanced Science, EarlyView.Cholesterol‐enriched plasma membranes in hepatocellular carcinoma impede drug penetration. Cholesterol (+)‐liposomes act as membrane‐specific detergents, extracting cholesterol and reducing barrier function without cytotoxicity. Following endocytosis, cholesterol transfers from endosomes to liposomes and is metabolized in the ER.
Chansik Kim +9 more
wiley +1 more source
Advanced Science, EarlyView.A minimally invasive optical window enables stable, long‐term imaging of peripheral nerves in vivo at single‐axon resolution. Dynamic processes of degeneration, regeneration, and cellular remodeling are visualized across multiple time scales within the same nerve region, providing a unique platform to study a variety of anatomical and molecular events ...
Igor D. Luzhansky +15 more
wiley +1 more source
Advanced Intelligent Discovery, EarlyView.Sequential multicolor fluorescence imaging in dynamic microsystems is constrained by acquisition speed and excitation dose. This study introduces a real‐time framework to reconstruct spectrally separated channels from reduced cross‐channel acquisitions (frames containing mixed spectral contributions).
Juan J. Huaroto +3 more
wiley +1 more source
Theory of fluorescence recovery after photobleaching measurements on cylindrical surfaces
Biophysical Journal, 1992 The theory of fluorescence recovery after photobleaching measurements of isotropic diffusion on a cylindrical surface is developed for Gaussian beam illumination centered perpendicular to an infinitely-long cylinder. A general analytical solution is obtained which is a function of the ratio of the cylindrical radius (r) to the beam exp [-2] radius ...
openaire +2 more sources
Cell Segmentation Beyond 2D—A Review of the State‐of‐the‐Art
Advanced Intelligent Discovery, EarlyView.Cell segmentation underpins many biological image analysis tasks, yet most deep learning methods remain limited to 2D despite the inherently 3D nature of cellular processes. This review surveys segmentation approaches beyond 2D, comparing 2.5D and fully 3D methods, analyzing 31 models and 32 volumetric datasets, and introducing a unified reference ...
Fabian Schmeisser +6 more
wiley +1 more source
A fluorescence recovery after photobleaching protocol to measure surface diffusion of DAGLα in primary cultured cortical mouse neurons. [PDF]
STAR Protoc, 2022 Yoon S, Penzes P.
europepmc +1 more source
Correction to: Fluorescence recovery after photobleaching: analyses of cyanobacterial phycobilisomes reveal intrinsic fluorescence recovery. [PDF]
Mar Life Sci Technol, 2021 Zhang N +6 more
europepmc +1 more source
Parameter estimation in fluorescence recovery after photobleaching: quantitative analysis of protein binding reactions and diffusion. [PDF]
J Math Biol, 2021 Williamson DE +4 more
europepmc +1 more source
Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells. [PDF]
STAR Protoc, 2021 Tingey M, Li Y, Yang W.
europepmc +1 more source
Marine silicon for biomedical sustainability
BMEMat, EarlyView.Schematic illustrating marine silicon for biomedical engineering. Abstract Despite momentous divergence from oceanic origin, human beings and marine organisms exhibit elemental homology through silicon utilization. Notably, silicon serves as a critical constituent in multiple biomedical processes.
Yahui Han +3 more
wiley +1 more source