Results 51 to 60 of about 55,338 (292)
SUMOylation of NaV1.2 channels mediates the early response to acute hypoxia in central neurons. [PDF]
, 2016 The mechanism for the earliest response of central neurons to hypoxia-an increase in voltage-gated sodium current (INa)-has been unknown. Here, we show that hypoxia activates the Small Ubiquitin-like Modifier (SUMO) pathway in rat cerebellar granule ...
Goldstein, Steve An +2 more
core +2 more sources
Data in Brief, 2016 The presented data provides additional information about the assessment of affinity purified nicotinic acetylcholine receptor (nAChR) rich membrane solubilized with long chain (16 saturated carbons) lysophospholipid with glycerol headgroup (LFG-16).
Luis F. Padilla-Morales +4 more
doaj +1 more source
Modern fluorescent techniques to investigate the mechanisms of lymphocyte activation [PDF]
The Ukrainian Biochemical Journal, 2015 Fluorescent proteins are promising tools for studying intracellular signaling processes in lymphocytes. This brief review summarizes fluorescence-based imaging techniques developed in recent years and discusses new methodological advances, such as ...
G. A. Liubchenko +2 more
doaj +1 more source
Osteoarthritis and Cartilage Open, 2020 Summary: Objective: Articular cartilage is an avascular tissue. Accordingly, diffusivity represents a fundamental transport mechanism for nutrients and other molecular signals regulating its cell metabolism and maintenance of the extracellular matrix ...
Francesco Travascio +2 more
doaj +1 more source
Scientific Reports, 2017 Reduction of NAD+ by dehydrogenase enzymes to form NADH is a key component of cellular metabolism. In cellular preparations and isolated mitochondria suspensions, enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP) of NADH has been ...
Angel Moreno +3 more
semanticscholar +1 more source
Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes [PDF]
PeerJ, 2020 Background In this study, we investigated the effect of the mechanical loading history on the expression of receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) in MLO-Y4 osteocyte-like cells.
Ziyi Wang +7 more
doaj +2 more sources
Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy [PDF]
, 2011 Modern fluorescence microscopy applications go along with increasing demands for the employed fluorescent dyes. In this work, we compared antifading formulae utilizing a recently developed reducing and oxidizing system (ROXS) with commercial antifading ...
Aitken +44 more
core +5 more sources
Fluorescence Recovery after Photobleaching: The Case of Anomalous Diffusion [PDF]
Biophysical Journal, 2008 The method of FRAP (fluorescence recovery after photobleaching), which has been broadly used to measure lateral mobility of fluorescent-labeled molecules in cell membranes, is formulated here in terms of continuous time random walks (CTRWs), which offer both analytical expressions and a scheme for numerical simulations.
Lubelski, Ariel, Klafter, Joseph
openaire +2 more sources
Calibrating evanescent-wave penetration depths for biological TIRF microscopy
, 2019 Roughly half of a cells proteins are located at or near the plasma membrane. In this restricted space the cell senses its environment, signals to its neighbors and ex-changes cargo through exo- and endocytotic mechanisms.
Becherer, Ute +4 more
core +1 more source
Unbreakable codes in electrospun fibers : digitally encoded polymers to stop medicine counterfeiting [PDF]
, 2010 Fluorescent polymer solutions can easily be electrospun into micrometer-sized fibers and subsequently encoded with long lasting digital codes by a photobleaching process.
Braeckmans, Kevin +9 more
core +2 more sources