Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and Photoactivation of cyan fluorescent proteins at YFP-Excitation [PDF]
, 2011 Background The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking specific proteins or cells
Johannes Schmid, Naila Malkani
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Multiphoton fluorescence recovery after photobleaching in bounded systems [PDF]
Physical Review E, 2011 Multiphoton fluorescence recovery after photobleaching (MP-FRAP) is a laser microscopy technique used to measure diffusion coefficients of macromolecules in biological systems. The three-dimensional resolution and superior depth penetration within scattering samples offered by MP-FRAP make it an important tool for investigating both in vitro and in ...
Kelley D, Sullivan, Edward B, Brown
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Transport between im/mobile fractions shapes the speed and profile of cargo distribution in neurons
Biophysical Reports, 2022 Neuronal function requires continuous distribution of ion channels and other proteins throughout large cell morphologies. Protein distribution is complicated by immobilization of freely diffusing subunits such as on lipid rafts or in postsynaptic ...
Adriano A. Bellotti +3 more
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Fuorescent dye diffusion coefficient measurement in murine brain tissues using the method of fluorescence recovery after photobleaching [PDF]
Известия Саратовского университета. Новая серия: ФизикаBackground and Objectives: The molecular transport in the brain tissue is yet to be fully studied and presents great interest to the researchers in the fields of biophysics and biomedicine. Diffusion and molecular transport processes in brain tissues are
Dubrovsky, Alexander Ilyich +4 more
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A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion
Nature Communications, 2021 It is challenging to quantify anisotropic diffusion in biological systems. Here the authors report light-sheet imaging-based Fourier transform fluorescence recovery after photobleaching (LiFT-FRAP) to noninvasively determine 3D diffusion tensors of ...
Peng Chen +9 more
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Journal of Innovative Optical Health Sciences, 2021 Fluorescence recovery after photobleaching (FRAP) and single particle tracking (SPT) techniques determine the diffusion coefficient from average diffusive motion of high-concentration molecules and from trajectories of low-concentration single molecules,
Donghee Lee +2 more
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eLife, 2020 Wnt3 proteins are lipidated and glycosylated signaling molecules that play an important role in zebrafish neural patterning and brain development. However, the transport mechanism of lipid-modified Wnts through the hydrophilic extracellular environment ...
Sapthaswaran Veerapathiran +6 more
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Fluorescence recovery after photobleaching on the confocal laser-scanning microscope: generalized model without restriction on the size of the photobleached disk [PDF]
, 2011 Fluorescence recovery after photobleaching (FRAP) carried out on a confocal laser-scanning microscope (CLSM) performs well for photobleached disks that are large compared to the resolution of the bleaching beam.
Ameloot, Marcel +6 more
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Fluorescence recovery after photobleaching: direct measurement of diffusion anisotropy [PDF]
Biomechanics and Modeling in Mechanobiology, 2020 Fluorescence recovery after photobleaching (FRAP) is a widely used technique for studying diffusion in biological tissues. Most of the existing approaches for the analysis of FRAP experiments assume isotropic diffusion, while only a few account for anisotropic diffusion. In fibrous tissues, such as articular cartilage, tendons and ligaments, diffusion,
Hashlamoun, Kotaybah +5 more
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