Results 11 to 20 of about 22,069 (203)
Fluorescence recovery after photobleaching: analyses of cyanobacterial phycobilisomes reveal intrinsic fluorescence recovery. [PDF]
Mar Life Sci Technol, 2021 Fluorescence recovery after photobleaching (FRAP) has been used to study the dynamics of the cyanobacterial photosynthesis apparatus since 1997. Fluorescence recovery of cyanobacteria during FRAP was conventionally interpreted as a result of phycobilisome (PBS) diffusion on the surface of the thylakoid membrane.
Zhang N +6 more
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Single- and two-photon fluorescence recovery after photobleaching. [PDF]
Cold Spring Harb Protoc, 2015 Fluorescence recovery after photobleaching (FRAP) is a microscopy technique for measuring the kinetics of fluorescently labeled molecules and can be applied both in vitro and in vivo for two- and three-dimensional systems. This introduction discusses the three basic FRAP methods: traditional FRAP, multiphoton FRAP (MPFRAP), and FRAP with spatial ...
Sullivan KD, Majewska AK, Brown EB.
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Analysis of Active Transport by Fluorescence Recovery after Photobleaching. [PDF]
Biophys J, 2017 Fluorescence recovery after photobleaching (FRAP) is a well-established experimental technique to study binding and diffusion of molecules in cells. Although a large number of analytical and numerical models have been developed to extract binding and diffusion rates from FRAP recovery curves, active transport of molecules is typically not included in ...
Ciocanel MV +4 more
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Analysis of Binding Reactions by Fluorescence Recovery after Photobleaching [PDF]
Biophysical Journal, 2004 Fluorescence recovery after photobleaching (FRAP) is now widely used to investigate binding interactions in live cells. Although various idealized solutions have been identified for the reaction-diffusion equations that govern FRAP, there has been no comprehensive analysis or systematic approach to serve as a guide for extracting binding information ...
Sprague, Brian L. +3 more
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Effects of Organelle Shape on Fluorescence Recovery after Photobleaching [PDF]
Biophysical Journal, 2005 The determination of diffusion coefficients from fluorescence recovery data is often complicated by geometric constraints imposed by the complex shapes of intracellular compartments. To address this issue, diffusion of proteins in the lumen of the endoplasmic reticulum (ER) is studied using cell biological and computational methods.
Sbalzarini IF +3 more
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Multiphoton fluorescence recovery after photobleaching in bounded systems [PDF]
Physical Review E, 2011 Multiphoton fluorescence recovery after photobleaching (MP-FRAP) is a laser microscopy technique used to measure diffusion coefficients of macromolecules in biological systems. The three-dimensional resolution and superior depth penetration within scattering samples offered by MP-FRAP make it an important tool for investigating both in vitro and in ...
Kelley D, Sullivan, Edward B, Brown
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Fluorescence Recovery after Photobleaching: The Case of Anomalous Diffusion [PDF]
Biophysical Journal, 2008 The method of FRAP (fluorescence recovery after photobleaching), which has been broadly used to measure lateral mobility of fluorescent-labeled molecules in cell membranes, is formulated here in terms of continuous time random walks (CTRWs), which offer both analytical expressions and a scheme for numerical simulations.
Lubelski, Ariel, Klafter, Joseph
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Fluorescence Recovery after Photobleaching in Ultrathin Polymer Films
Macromolecular Chemistry and Physics, 2017 Abstract Fluorescence recovery after photobleaching (FRAP) is a widely used technique to study the transport of molecules in biological systems. Recently, FRAP has been used to study molecular transport in polyelectrolyte multilayers (PEMs).
Praneet Prakash +2 more
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A Method Improving the Accuracy of Fluorescence Recovery after Photobleaching Analysis [PDF]
Biophysical Journal, 2008 Fluorescence recovery after photobleaching has been an established technique of quantifying the mobility of molecular species in cells and cell membranes for more than 30 years. However, under nonideal experimental conditions, the current methods of analysis still suffer from occasional problems; for example, when the signal/noise ratio is low, when ...
Jönsson, Peter +3 more
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Fluorescence recovery after photobleaching: direct measurement of diffusion anisotropy [PDF]
Biomechanics and Modeling in Mechanobiology, 2020 Fluorescence recovery after photobleaching (FRAP) is a widely used technique for studying diffusion in biological tissues. Most of the existing approaches for the analysis of FRAP experiments assume isotropic diffusion, while only a few account for anisotropic diffusion. In fibrous tissues, such as articular cartilage, tendons and ligaments, diffusion,
Hashlamoun, Kotaybah +5 more
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