EasyFRAP-web: a web-based tool for the analysis of fluorescence recovery after photobleaching data. [PDF]
Nucleic Acids Res, 2018 Understanding protein dynamics is crucial in order to elucidate protein function and interactions. Advances in modern microscopy facilitate the exploration of the mobility of fluorescently tagged proteins within living cells.
Koulouras G +5 more
europepmc +4 more sources
Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy. [PDF]
Biophys Physicobiol, 2018 Fluorescence recovery after photobleaching (FRAP) enables characterization of quantitative dynamic properties such as diffusion coefficients of fluorescent molecules in living cells by analyzing the recovery of fluorescence intensity after photobleaching
Kitamura A, Kinjo M.
europepmc +4 more sources
Nasal epithelial cell culture fluorescence recovery after photobleaching predicts cystic fibrosis therapeutic response [PDF]
ERJ Open Research, 2022 Background Human nasal epithelial (HNE) cells can be sampled noninvasively and cultured to provide a model of the airway epithelium that reflects cystic fibrosis (CF) pathophysiology.
Timothy E. Corcoran +12 more
doaj +2 more sources
Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching. [PDF]
Biophys J, 2019 Cells contain numerous membraneless organelles that assemble by intracellular liquid-liquid phase separation. The viscous properties and associated biomolecular mobility within these condensed phase droplets, or condensates, are increasingly recognized ...
Taylor NO +3 more
europepmc +2 more sources
FRAP analysis: accounting for bleaching during image capture. [PDF]
PLoS ONE, 2012 The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a ...
Jun Wu +3 more
doaj +4 more sources
Analysis of Binding Reactions by Fluorescence Recovery after Photobleaching [PDF]
Biophysical Journal, 2004 Fluorescence recovery after photobleaching (FRAP) is now widely used to investigate binding interactions in live cells. Although various idealized solutions have been identified for the reaction-diffusion equations that govern FRAP, there has been no comprehensive analysis or systematic approach to serve as a guide for extracting binding information ...
Sprague, Brian L. +3 more
openaire +3 more sources
Defining the Diffusion in Model Membranes Using Line Fluorescence Recovery after Photobleaching [PDF]
Membranes, 2020 In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging.
Jakob L. Kure +4 more
doaj +2 more sources
Fluorescence recovery after photobleaching: analyses of cyanobacterial phycobilisomes reveal intrinsic fluorescence recovery. [PDF]
Mar Life Sci Technol, 2021 Fluorescence recovery after photobleaching (FRAP) has been used to study the dynamics of the cyanobacterial photosynthesis apparatus since 1997. Fluorescence recovery of cyanobacteria during FRAP was conventionally interpreted as a result of phycobilisome (PBS) diffusion on the surface of the thylakoid membrane.
Zhang N +6 more
europepmc +3 more sources
Analysis of Exosome Transfer in Mammalian Cells by Fluorescence Recovery after Photobleaching [PDF]
Bio-Protocol, 2018 During the course of evolution, prokaryote and eukaryote cells have developed elegant and to some extent analogous strategies to communicate with each other and to adapt to their surrounding environment.
Hernán González-King +9 more
doaj +2 more sources
Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability [PDF]
Nature Communications, 2016 Measuring the size distribution of nanomaterials in biological fluids is crucial to understand their properties in vivo. Here, the authors apply fluorescence recovery after photobleaching to measure protein aggregation in serum and to study permeability ...
Ranhua Xiong +8 more
doaj +3 more sources