Determining vitreous viscosity using fluorescence recovery after photobleaching. [PDF]
PLoS ONE, 2022 PurposeVitreous humor is a complex biofluid whose composition determines its structure and function. Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
Nishanthan Srikantha +6 more
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The Utility of Fluorescence Recovery after Photobleaching (FRAP) to Study the Plasma Membrane [PDF]
Membranes, 2023 The plasma membrane of mammalian cells is involved in a wide variety of cellular processes, including, but not limited to, endocytosis and exocytosis, adhesion and migration, and signaling.
Charles A. Day, Minchul Kang
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Recent Advances in Fluorescence Recovery after Photobleaching for Decoupling Transport and Kinetics of Biomacromolecules in Cellular Physiology. [PDF]
Polymers (Basel), 2022 Among the new molecular tools available to scientists and engineers, some of the most useful include fluorescently tagged biomolecules. Tools, such as green fluorescence protein (GFP), have been applied to perform semi-quantitative studies on biological ...
Cai N +5 more
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Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics. [PDF]
PLoS Computational Biology, 2022 Fluorescence Recovery After Photobleaching (FRAP) has been extensively used to understand molecular dynamics in cells. This technique when applied to soluble, globular molecules driven by diffusion is easily interpreted and well understood.
J C Dallon +5 more
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Analysis of Active Transport by Fluorescence Recovery after Photobleaching. [PDF]
Biophys J, 2017 Fluorescence recovery after photobleaching (FRAP) is a well-established experimental technique to study binding and diffusion of molecules in cells. Although a large number of analytical and numerical models have been developed to extract binding and diffusion rates from FRAP recovery curves, active transport of molecules is typically not included in ...
Ciocanel MV +4 more
europepmc +5 more sources
Single- and two-photon fluorescence recovery after photobleaching. [PDF]
Cold Spring Harb Protoc, 2015 Fluorescence recovery after photobleaching (FRAP) is a microscopy technique for measuring the kinetics of fluorescently labeled molecules and can be applied both in vitro and in vivo for two- and three-dimensional systems. This introduction discusses the three basic FRAP methods: traditional FRAP, multiphoton FRAP (MPFRAP), and FRAP with spatial ...
Sullivan KD, Majewska AK, Brown EB.
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Protein Synthesis Rate Assessment by Fluorescence Recovery after Photobleaching (FRAP) [PDF]
Bio-Protocol, 2017 Currently available biochemical methods cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. Here, we describe a non-invasive method for monitoring protein synthesis in single cells or tissues with intrinsically
Nikos Kourtis, Nektarios Tavernarakis
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Monitoring condensate dynamics in S. cerevisiae using fluorescence recovery after photobleaching [PDF]
STAR Protocols, 2022 Summary: This protocol describes the use of fluorescence recovery after photobleaching (FRAP) to investigate the dynamics of Matrin-3 (MATR3) condensates in live budding yeast.
Macy L. Sprunger, Meredith E. Jackrel
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Parameter Identifiability in PDE Models of Fluorescence Recovery After Photobleaching [PDF]
Bulletin of Mathematical Biology, 2023 Identifying unique parameters for mathematical models describing biological data can be challenging and often impossible. Parameter identifiability for partial differential equations models in cell biology is especially difficult given that many established \textit{in vivo} measurements of protein dynamics average out the spatial dimensions.
Maria-Veronica Ciocanel +6 more
openaire +5 more sources
Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Molecules, 2012 Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Gregor P. C. Drummen +2 more
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