Long-term molecular turnover of actin stress fibers revealed by advection-reaction analysis in fluorescence recovery after photobleaching. [PDF]
PLoS ONE, 2022 Fluorescence recovery after photobleaching (FRAP) is a versatile technique to evaluate the intracellular molecular exchange called turnover. Mechanochemical models of FRAP typically consider the molecular diffusion and chemical reaction that ...
Takumi Saito +2 more
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Sensors, 2010 This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies.
Gamal Rayan +4 more
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Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells [PDF]
STAR Protocols, 2021 Summary: Single-molecule fluorescence recovery after photobleaching (smFRAP) is a newly developed technique that combines single-molecule super-resolution microscopy and traditional FRAP microscopy.
Mark Tingey, Yichen Li, Weidong Yang
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Assessing cellular efficacy of bromodomain inhibitors using fluorescence recovery after photobleaching. [PDF]
Epigenetics Chromatin, 2014 BackgroundAcetylation of lysine residues in histone tails plays an important role in the regulation of gene transcription. Bromdomains are the readers of acetylated histone marks, and, consequently, bromodomain-containing proteins have a variety of ...
Philpott M +9 more
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Fluorescence Recovery after Photobleaching in Ultrathin Polymer Films
Macromolecular Chemistry and Physics, 2017 Abstract Fluorescence recovery after photobleaching (FRAP) is a widely used technique to study the transport of molecules in biological systems. Recently, FRAP has been used to study molecular transport in polyelectrolyte multilayers (PEMs).
Praneet Prakash +2 more
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Effects of Organelle Shape on Fluorescence Recovery after Photobleaching [PDF]
Biophysical Journal, 2005 The determination of diffusion coefficients from fluorescence recovery data is often complicated by geometric constraints imposed by the complex shapes of intracellular compartments. To address this issue, diffusion of proteins in the lumen of the endoplasmic reticulum (ER) is studied using cell biological and computational methods.
Sbalzarini, Ivo F. +3 more
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Investigating the effect of proteoglycan 4 on hyaluronan solution properties using confocal fluorescence recovery after photobleaching [PDF]
BMC Musculoskeletal Disorders, 2019 Background The objective of this study was to use confocal fluorescence recovery after photobleaching (FRAP) to examine the specific and dose-dependent effect of proteoglycan 4 (PRG4) on hyaluronan (HA) solutions of different molecular weight; and assess
Adam K. Bloom +4 more
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Radial Profile-Based Quantification of Centrosomal Proteins [PDF]
Bio-ProtocolCentrosomes are dynamic organelles critical for mitotic spindle assembly and cilia formation. Here, I describe a protocol for quantifying relative centrosomal protein abundance in Drosophila melanogaster embryos using radial profile analysis of ...
Alan Wainman
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UV Light Induced Fluorescence Recovery of GFP After Photobleaching in Microscopy Imaging
Image Analysis and Stereology, 2022 Fluorescence microscopy has become one of the most important tools for biologists to visualize and study organelles and molecules in a cell. Fluorescent markers are used to visualize specific molecules.
Griša G. Prinčič +4 more
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Photobleaching and Fluorescence Recovery of RPE Bisretinoids. [PDF]
PLoS ONE, 2015 The autofluorescence of the retina that originates primarily from lipofuscin fluorophores in retinal pigment epithelial cells, is observed to undergo photobleaching during the acquisition of fundus autofluorescence images.
Zhao Liu +3 more
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