Results 51 to 60 of about 262,308 (303)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Quantitative detection of pharmaceuticals using a combination of paper microfluidics and wavelength modulated Raman spectroscopy

, 2015
Raman spectroscopy has proven to be an indispensable technique for the identification of various types of analytes due to the fingerprint vibration spectrum obtained.
Derek Craig, Kishan Dholakia (426528), Michael Mazilu, Michael Mazilu (426524), Craig, Derek, Mazilu, Michael, Dholakia, Kishan, Derek Craig (290904), Derek Craig, Kishan Dholakia   +9 more
core   +1 more source

First Design of a Contact Lens for Diagnosis of Dehydration

Biosensors
Dehydration is a serious medical problem for elderly patients and young children. The most widely used diagnostics are measurements of sodium ion (Na+) and potassium ion (K+) in blood serum.
Kundan Sivashanmugan, Reece E. Albert, Joseph R. Lakowicz   +2 more
doaj   +1 more source

Application of Curve Fitting and Radial Basis Function in the Quantitative Analysis of Molecular Fluorescence Spectroscopy

Chemical Engineering Transactions, 2018
The fluorescence of Lactobacillus plantarum solution was characterized by two-dimensional fluorescence spectroscopy and three-dimensional fluorescence spectroscopy and the quantitative detection of the fluorescence peak intensity of Lactobacillus ...
Huan Guo
doaj   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Structure of the Cytoplasmic Loop between Putative Helices II and III of the Mannitol Permease of Escherichia coli: A Tryptophan and 5-Fluorotryptophan Spectroscopy Study [PDF]

, 2008
In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EIImtl, are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy.
Bokhove, Marcel,, Broos, Jaap,, Erwin P. P. Vos, Hesp, Ben H., Vos, Erwin P. P., Vos, Erwin P.P.,, Hesp, Ben H.,, Broos, Jaap, Bokhove, Marcel, Ben H. Hesp, Jaap Broos, Marcel Bokhove   +11 more
core   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Fluorescence fluctuation analysis of Arabidopsis thaliana somatic embryogenesis receptor-like kinase and brassinosteroid insensitive 1 receptor ologomerization.

, 2008
Receptor kinases play a key role in the cellular perception of signals. To verify models for receptor activation through dimerization, an experimental system is required to determine the precise oligomerization status of proteins within living cells ...
Vries, S.C., de, Russinova, Eugenia, Visser, Antonie J.W.G., Shah, K., Vries, S.C., de Vries, Sacco C, Hink, M.A., de Vries, S.C., Shah, Khalid, Hink, Mark A, Visser, A.J.W.G., Russinova, E.T., Visser, Antonie JWG, Hink, Mark A., de Vries, Sacco C., Russinova, E.   +15 more
core   +1 more source

A versatile standard for bathochromic fluorescence based on intramolecular FRET. [PDF]

, 2011
A perylene and a terrylene tetracarboxylic bisimide dyad was prepared in which an efficient energy transfer from the former to the latter is observed. The absorption spectrum of this compound covers a broad range.
Rosenbaum, Erik, A Andreas Walter, Lennart B.-Å. Johansson, Rosenbaum, Erik,, Andreas Walter, Walter, Andreas, A Erik Rosenbaumb, Johansson, Lennart B.-A., Erik Rosenbaum, Heinz Langhals, Johansson, Lennart B-Å,, Langhals, Heinz, Lennart B. -å. Johanssonb   +12 more
core   +1 more source

Xanthines Studied via Femtosecond Fluorescence Spectroscopy

Molecules, 2016
Xanthines represent a wide class of compounds closely related to the DNA bases adenine and guanine. Ubiquitous in the human body, they are capable of replacing natural bases in double helices and give rise to four-stranded structures. Although the use of
Pascale Changenet-Barret, Lajos Kovács, Dimitra Markovitsi, Thomas Gustavsson   +3 more
doaj   +1 more source