Indirect fluorescent antibody technique in the serology of Toxoplasma gondii [PDF]
The indirect fluorescent antibody technique has been used to titrate patients' antibody against Toxoplasma gondii. Rigorous tests of the immunological validity of the method were made. The reactions of the gamma globulin of human immune serum with Toxoplasma gondii and fluorescent rabbit anti-human gamma globulin were thus shown to be specific ...
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The subnuclear localization of tRNA ligase in yeast [PDF]
Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
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Detection of lumpy skin disease virus in cattle using real-time polymerase chain reaction and serological diagnostic assays in different governorates in Egypt in 2017 [PDF]
Background and Aim: Lumpy skin disease (LSD), is a highly infectious viral disease of cattle, caused by LSD virus (LSDV) which belongs to the genus Capripoxvirus of family Poxviridae. In the summer of 2017, skin lesions suggestive of LSD were observed in
Gamil Sayed Gamil Zeedan +5 more
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Measurement of Antibodies to Machupo Virus by the Indirect Fluorescent Technique
Bolivian hemorrhagic fever (BHF) was first recognized in the Province of Beni, Bolivia, in 1959 (1), and cases have continued to occur since then. Recent outbreaks have confirmed the fact that clinical manifestations can be quite nonspecific. Laboratory diagnosis is handicapped by a scarcity of facilities in the endemic region, as well as by the hazard
C J, Peters, P A, Webb, K M, Johnson
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Detection of laser-UV microirradiation-induced DNA photolesions by immunofluorescent staining [PDF]
A low-power laser-UV microbeam of wave-length 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed ...
Cremer, Christoph +3 more
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Indirect fluorescent-antibody technique for serological diagnosis of La Crosse (California) virus infections [PDF]
A clinically relevant indirect fluorescent-antibody technique (IFA) was developed for the serological diagnosis of La Crosse virus infections. The IFA (67%) was as sensitive as the hemagglutination inhibition (58%) and neutralization (58%) tests in the detection of antibodies in acute-phase specimens.
B J, Beaty +6 more
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Effect of preoperative thoracic duct drainage on canine kidney transplantation [PDF]
Chronic drainage of the thoracic duct to the esophagus was developed in dogs, and its efficacy in immunomodulation was tested using kidney transplantation. Compared to 9.7 days in the control, the mean animal survival was prolonged to 9.9 days, 17.8 days,
Archimbaud JP +43 more
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Performance evaluation of an indirect immunofluorescence kit for the serological diagnosis of dengue
Objective: To evaluate the performance of indirect immunofluorescence for serological diagnosis of dengue virus in a population with high prevalence of arboviruses. Methods: Two-hundred serum samples from patients with clinical suspicion of dengue fever
Karina Emy Arai +4 more
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Idiopathic inflammatory myopathies (IIM) are a heterogeneous group of systemic autoimmune rheumatic disorders in which chronic inflammation of skeletal muscle leads to muscle weakness. Many other organs, including the skin, heart, lungs, and joints, may
Cláudia Pinto Oliveira +13 more
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Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil
Neospora caninum is an obligate intracellular parasite that can infect many domestic and wild animals, including birds. These animals are important sources for monitoring of environmental contamination, as they could become infected through sporulated ...
João Pedro Sasse +6 more
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