Objective: Determine the frequency of infection and disease by Rickettsia spp. of the spotted fever group in febrile patients from Urabá Antioquia attended by hospital centers of the region. Methods: Patients from nine health institutions of the Urabá
Gil Lora, Erica Johana +4 more
doaj +1 more source
The subnuclear localization of tRNA ligase in yeast [PDF]
Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
core +1 more source
Detection of lumpy skin disease virus in cattle using real-time polymerase chain reaction and serological diagnostic assays in different governorates in Egypt in 2017 [PDF]
Background and Aim: Lumpy skin disease (LSD), is a highly infectious viral disease of cattle, caused by LSD virus (LSDV) which belongs to the genus Capripoxvirus of family Poxviridae. In the summer of 2017, skin lesions suggestive of LSD were observed in
Gamil Sayed Gamil Zeedan +5 more
doaj +1 more source
Tracking elusive cargo: Illuminating spatio-temporal type 3 effector protein dynamics using reporters [PDF]
Type 3 secretion systems (T3SS) form an integral part of the arsenal of many pathogenic bacteria. These injection machines, together with their cargo of subversive effector proteins are capable of manipulating the cellular environment of the host in ...
Connolly, James P.R. +2 more
core +1 more source
Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future [PDF]
Serological techniques commonly used to quantify influenza-specific antibodies include the Haemagglutination Inhibition (HI), Single Radial Haemolysis (SRH) and Virus Neutralization (VN) assays.
Callow +30 more
core +3 more sources
Indirect fluorescent antibody techniques for demonstration of trout viruses and corresponding antibody. [PDF]
Two variations of the indirect fluorescent antibody technique (FAT) have been utilized in the work concerning two important virus diseases of trout, viral haemorrhagic septicaemia (VHS) and infectious pancreatic necrosis (IPN). A “two layer” indirect FAT allowed demonstration of the respective viruses in cell cultures and a “three layer” indirect FAT ...
openaire +3 more sources
Performance evaluation of an indirect immunofluorescence kit for the serological diagnosis of dengue
Objective: To evaluate the performance of indirect immunofluorescence for serological diagnosis of dengue virus in a population with high prevalence of arboviruses. Methods: Two-hundred serum samples from patients with clinical suspicion of dengue fever
Karina Emy Arai +4 more
doaj +2 more sources
Comparison of rabies humoral antibody titers in rabbits and humans by indirect radioimmunoassay, rapid-fluorescent-focus-inhibition technique, and indirect fluorescent-antibody assay [PDF]
Rabies humoral antibodies were induced in eight New Zealand rabbits by a single intramuscular injection of inactivated suckling mouse brain rabies vaccine. The primary response to immunization was measured in blood samples taken at selected intervals for 6 months.
T K, Lee, H D, Hutchinson, D W, Ziegler
openaire +2 more sources
Idiopathic inflammatory myopathies (IIM) are a heterogeneous group of systemic autoimmune rheumatic disorders in which chronic inflammation of skeletal muscle leads to muscle weakness. Many other organs, including the skin, heart, lungs, and joints, may
Cláudia Pinto Oliveira +13 more
doaj +1 more source
Effects of LP-MOCVD prepared TiO2 thin films on the in vitro behavior of gingival fibroblasts [PDF]
We report on the in vitro response of human gingival fibroblasts (HGF-1 cell line) to various thin films of titanium dioxide (TiO2) deposited on titanium (Ti) substrates by low pressure metal-organic chemical vapor deposition (LP-MOCVD).
Cimpean, A. +3 more
core +2 more sources

